x-gal 中文意思是什麼
x-gal
解釋
苷酶-
Fixed with gluteraldehyde, stained with ph 7. 4 phosphate buffered x - gal dye solution, observed by reverse microscope, the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus
病毒感染后的8h ， 12h ， 16h ， 20h提取中腸組織，按常規石蠟包埋切片進行洗滌、透明、浸蠟、包埋、切片、粘片等操作程序，製成連續切片，在倒置顯微鏡下逐一進行觀察計數。 -
Then the linked products were transformed into the high competent cell of e. coli dh5a. based on - complementation of the detective - galactosidase, positive recombinant clone were screened from x - gal plate
從引物和基因序列的比對分析結果看， zhyf006序列與上下游引物的配對比例分別為s4 -
A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。 -
After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。 -
Thus the objective of this study is to find some ways to improve the efficiency of cell therapy, that is. to optimize the microenvironment of nscs and in turn prompt them to functinally repair abnormal cns. in the first part, optimization of x - gal staining method, which is correlated with ph, incubating time, perfusion and some other parameters, was successfully got and used in the subsequent experiment, viz, comparing the behaviours of primary nscs and immortal nsc line after transplantation
鑒於此，為尋求各種方法提高移植效率，改善外源nscs存活及分化，進而促進其實現功能性替代，為臨床nscs治療提供理論基礎，本實驗進行了以下三部分的研究：實驗第一部分研究了以lacz作為移植細胞標志基因時x - gal染色的影響因素，並得出排除非特異背景的優化條件。
分享友人