yeast expression system 中文意思是什麼

yeast expression system 解釋
酵母表達系統
  • yeast : n. 1. 酵母（菌）。2. 發酵粉；酵母餅，酵母片。3. 泡，泡沫。4. 動亂，激動。
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  • system : n 1 體系，系統；分類法；組織；設備，裝置。2 方式；方法；作業方法。3 制度；主義。4 次序，規律。5 ...
  1. This paper summarizes the commonly used methods for the research of gene function of filamentous fungi, such as transposon tagging, gene knockout, rna interference, over - expression and yeast hybrid system, and provides a discussion on the advantages and disadvantages of those methods

    本文總結了目前絲狀真菌基因功能研究中常用的方法，如轉座子標簽法、基因敲除技術、 rna干擾、超表達及酵母雜交系統等，並對各方法在絲狀真菌研究中的優缺點進行了闡述。
  2. Recently, the expression system of methylotrophic yeast has been developed quickly. as a result of its many advantages, such as high expression, high stability, high secretion and so on, many kinds of protein, including animal, plant, microorganism proteins, have been expressed by this system in the world, but we have not seen any report about yeast expression of poifn - a until now, this research is the first example in the world that poifn - a is expressed by methylotrophic yeast system

    近年來，甲醇營養型酵母表達系統的研究得到迅速發展，因其具有高表達、高穩定、高分泌、能對表達的外源蛋白進行正確的折疊、加工，利於下游分離純化操作、能大規模發酵生產等諸多優點，國內外已經利用此系統表達了多種對人類有益的動植物及微生物活性蛋白，但對豬型干擾素的酵母表達還未見報導，本研究是國內外首次採用此系統表達豬型干擾素。
  3. Progress of methylotrophic yeast pichia pastoris expression system

    巴斯德畢赤酵母表達系統研究進展
  4. Northern blot and rt - pcr analysis verified that the expression level of ldplcl was induced in germinating pollen, whereas ldplc2 expression level did not change during pollen germinating process. the interaction between heterotrimeric g proteins and plc was detected in a yeast two - hybrid system

    Northern和rt - pcr分析，發現隨著花粉的萌發， ldplc2的轉錄活性提高，而ldplc1博士學位論文在萌發前後的轉錄水平沒有變化，推測至少ldplcz參與了對花粉萌發的調控。
  5. In our work, different hbv pres gene fragments were amplified by pcr, cloned into pgbkt ? vector in yeast two - hybrid system 3, and then transformed ah 109 yeast cells. the results showed that these pres expression products were nontoxic to yeast cells, but all self - activated the yeast two - hybrid system

    本研究將hbv前s區不同片斷進行擴增，克隆入酵母雙雜交系統3中的pgbkt7載體，轉化酵母細胞ah109 ，經檢測，證實了hbv前s區不同片斷對酵母細胞無毒性作用，但是都存在自激活作用。
  6. Human elongator complex serves a role in chromatin remodeling through modifying the lysine residues of histone h3 and h4 during transcription elongation in higher cells. the main results and conclusions are as followings. 1 using depletion strain of yeast as material, we have established a system for complementation test and gene expression analysis of human elongator complex

    4 、利用點突變和plasmidshuffle等技術構建了含h3k14一r 、 h4ks ~ r單突變和h3k14一側h4ks一r雙突變的e1p3乙菌株，通過功能互補實驗分析少凡p3對突變株的補償功能，證實酵母elongator可對h314k / h4sk進行乙酞化修飾。
  7. This paper reviews the recent advances in yeast expression systems used in recombinant protein drugs produced by gene engineering, such as the selection of yeast vector system and yeast expression strain, construction of multi - copy strain, the high yeast cell density culture, the recent situation of yeast system applied in expression gene, defect of yeast expression system and countermeasure, etc

    摘要本文主要從酵母載體系統的選擇、酵母表達宿主的選擇、多拷貝菌株的構建、高密度發酵培養酵母細胞、應用酵母系統表達外源基因的研究現狀、酵母表達系統的缺陷及對策等幾個方面綜述了近年來酵母表達體系在基因工程重組蛋白藥物開發方面的研究進展。
  8. Pichia pastoris gene expression system is a second generation expression system in yeast after the s. cerevisiae

    甲醇畢赤酵母pichiapastoris表達系統是繼釀酒酵母之後的第二代酵母表達系統。
  9. Methods : 1. expression and purification of recombinant human ctla4 extracellular domain : a 400bp dna fragment of ctla4 extracellular domain was obtained from the pctla4 / ig plasmid with genetical technique. then, this dna fragment was inserted into ppic9 plasmid to construct the single copy plasmid of yeast expression system ( ppic9 - ctla4125 ). furthermore, the multi - copy plasmid ( ppic9k - ctla4125 ) was constructed

    重組人ctla4胞外區蛋白在酵母gs115中的表達和純化：首先通過基因操作技術，從pctla4八g質粒中擴增400hp的ctla4胞外區片段；將ctla4胞外區片段插人ppicg質粒中獲取單拷貝酵母表達質粒ppicg ctla4125並進一步構建多拷貝酵母表達質粒ppicgk （ 。
  10. For the pure ple has the better activity, a procedure is designed in this research, that is to construct the engineering microorganism to express the ple efficiently. methylotrophic yeast expression system has been developed quickly recently. many proteins are expressed by it in foreign countries

    甲醇酵母表達系統是最近迅速發展起來的一種真核表達系統，它能以甲醇為唯一碳源大量合成蛋白質，已用於表達多種外源蛋白，被認為是最具發展前景的蛋白質生產工具之一。
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