yeast strain 中文意思是什麼
yeast strain
解釋
酵母菌株-
The result of using eight kinds of different yeast strain as biocatalyst reveals that baker ' s yeast is the best biocatalyst in this transformation
比較了八種酵母的生物轉化能力，發現在有機合成生物轉化中應用最廣泛的生物催化劑baker ' s酵母最適合催化該模型反應； 3 -
The brewing characteristics of shaoxing wine were presented in the following eleven aspects : the uniqueness of adjunct materials and the variety of different wine, the treatment of soaking rice at low temperature for a long time, the variety of fermentation state, the characteristics of rice wine broth, the variety and complexity of microorganisms, the uniqueness of inoculation and strain preservation, the process of mixed fermentation of yeast and lactic acid bacteria ( e. g. the concurrence of saccharification, yeast fermentation and lactic fermentation ), the over - mixing of different liquors, the high sterilization temperature, and the storage of produced wine
摘要該文從11個方面論述了紹興黃酒的釀造特點，即配料的特殊性和酒種的多樣性；低溫長時間浸米；發酵狀態的多樣性；黃酒醪的發酵特點；微生物的多樣性，復雜性；接種方式獨特；菌種保存方法獨特；酵母與乳酸桿菌協同作用的混合發酵并行的過程（即邊糖化、邊酵母發酵、邊乳酸發酵同時進行的三邊發酵） ；酒液勾兌；較高的滅菌溫度；成品酒的貯存。 -
1. the colony and microscopic characters of pt95 strain in 4 kinds of media, i. e. czapek yeast extract agar ( cya ), malt extract agar ( mea ), 25 % glycerol nitrate agar ( g25n ) and czapek agar ( ca ), were observed respectively
分別觀察了pt95菌株在查氏酵母膏瓊脂（ cya ） 、麥芽汁瓊脂（ mea ） 、 25甘油硝酸鹽瓊脂（ g25n ）和查氏瓊脂（ ca ）上的菌落特徵和個體形態特徵。 -
Addition of yeast extraction, or peptone or glucose could promote the growth and phenanthren degradation of zx4 strain at different degree
酵母膏、蛋白腖和葡萄糖等有機物的加入都可不同程度地促進zx4菌株的生長和菲降解。 -
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。 -
The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination
本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。 -
Transformation was done by electroporation. human fl extracellular domain cdna transformed to yeast host strain km71, then his + muts phenotype transformant was screened out and cultured in flasks, and rhfl was expressed under the induction of 0. 5 % methanol
我們提取了km71ppic9k - fl轉化菌株的基因組dna進行southern實驗，檢測目的基因的整和插入；提取總rna ，進行了northern實驗，檢測fl基因在轉化菌株中的表達。 -
The program of breeding the strain of yeast which can endure high temperature and have high productivity is carrying on all around the world
耐高溫高產酒精酵母的選育，是各國正在研究的重要方向。 -
Baker ' s yeast ( saccharomyces cerevisiae ) is a major strain of trehalose production
麵包酵母（ saccharomycescerevisiae ）是生產海藻糖的重要菌種。 -
Shake flask fermentation experiments showed that the expression of the intracellular vhb in the yeast strain pichia pastoris gs115 / ppic9m / ppic3. 5k improved monellin production. secreted monellin is about 0. 3g / l
Vhb在胞內的成功表達，使重組畢赤酵母高甜度monellin的分泌表達量提高了20 % ，達0 . 3g / l 。 -
Isolation and purification of modified yacs from yeast strain have been carried out by pulsed - field gel electrophoresis. the modified yacs are expected to generate plant artificial chromosome after being transferred into arabidopsis protoplast by liposome - mediated method
用脈沖電泳將經過同源重組的修飾的yac克隆分離出來通過脂質體轉化植物的原生質體，希望得到在植物細胞中穩定存在的植物人工染色體。 -
Studying on the effects of nitrogen sources, carbon sources and other influence factors has optimized the lipase fermentation conditions for the strain. the optimal medium composition for the lipase production is ( % ) : yeast extract 1
對菌株lz1進行產酶條件優化研究，發現碳源和氮源的種類，碳氮源比值以及細菌發酵的初始ph值對酶產量影響最大。 -
The mature gene of gloshedobin was inserted into the expression vector ppic9k. the yeast pichia pastoris strain gs115 was applied to study the expression
經sds - page ，親和層析及電鏡分析，表達產摘蟄物主要以可溶性的蛋白質形式存在於細胞質中。 -
The yeast strain 12y - 5, based on careful comparison with known yeast strains, was primarily identified as the trichosporon sp
認為該菌株可能是絲孢酵母屬（ trichosporon ）一新種。 -
In the present work, the strain 12y - 5 that should be of use for production of single cell protien was selected from 14 strains of yeast isolates
本文從14個酵母菌株中篩選出一株具有潛在工業應用價值的菌株12y - 5 。 -
Clarified juice, generally stored in a closed stainless steel tank, is inoculated with a selected yeast strain
澄清后的葡萄汁，一般儲存在封閉的不銹鋼罐中，用選定的酵母菌種進行接種。 -
The yeast strain 12y - 5 was identified at the species level using two yeast taxonomy systems published by. j. w. kreger - van rij ( 1984 ) [ 24 ] and j. a. barnett ( 1983 ). [ 19 ] in addition, the ability to assimilate several compounds as main sources of carbon and nitrogen was evaluated at the 500ml shake - flask and 3. 7l kfl bioreactor scales. effects of ph, temperature, concentration of dissolved oxygen and utilizable sugar on the growth rate of cells and the rate of the conversion of substrate into biomass were studied
進而用正交試驗法對該酵母生長的培養基配方及影響生長的重要理化因素進行了較為深入的研究，在此基礎上對影響生長的重要理化因素進行了寬范圍的測驗：並以搖瓶最佳的培養條件為基礎，在3 . 7升kfl -生物反應器上進行了放大試驗。 -
Human elongator complex serves a role in chromatin remodeling through modifying the lysine residues of histone h3 and h4 during transcription elongation in higher cells. the main results and conclusions are as followings. 1 using depletion strain of yeast as material, we have established a system for complementation test and gene expression analysis of human elongator complex
4 、利用點突變和plasmidshuffle等技術構建了含h3k14一r 、 h4ks ~ r單突變和h3k14一側h4ks一r雙突變的e1p3乙菌株，通過功能互補實驗分析少凡p3對突變株的補償功能，證實酵母elongator可對h314k / h4sk進行乙酞化修飾。 -
This paper reviews the recent advances in yeast expression systems used in recombinant protein drugs produced by gene engineering, such as the selection of yeast vector system and yeast expression strain, construction of multi - copy strain, the high yeast cell density culture, the recent situation of yeast system applied in expression gene, defect of yeast expression system and countermeasure, etc
摘要本文主要從酵母載體系統的選擇、酵母表達宿主的選擇、多拷貝菌株的構建、高密度發酵培養酵母細胞、應用酵母系統表達外源基因的研究現狀、酵母表達系統的缺陷及對策等幾個方面綜述了近年來酵母表達體系在基因工程重組蛋白藥物開發方面的研究進展。 -
The recombinant expression vector ppic3. 5kv with vgb gene was used to transform the pichia pastoris gs115 / ppic9m via electroporation, yeast strain pichia pastoris gs115 / ppic9m / ppic3. 5k containing the integrated monellin gene and vgb gene was obtained by g418 ( 0. 5mg / ml ) plate
進一步通過電擊轉化將vgb基因整合到重組酵母gs115 / ppic9m的基因組中，獲得了由高甜度monellin和vgb兩個基因同時整合的重組酵母gs115 / ppic9m / ppic3 . 5kv 。
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