yeast two-hybrid system 中文意思是什麼
yeast two-hybrid system
解釋
酵母雙雜交系統-
In order to figure out the machanism of transcription control by ap - 2 a and to find the partner proteins which derectly binding to ap - 2 a, a yeast two - hybrid system by using ap - 2 a as a bait was performed to screen the hela cell cdna library
為了闡明ap - 2確切的轉錄調控方式，找出與其直接相互作用的蛋白質因子，我們以ap - 2為餌蛋白通過酵母雙雜交對hela細胞的cdna文庫進行了篩選。 -
Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system
進一步通過rt . pcr克隆了ked樣蛋白編碼區全長cdna ，通過雙雜交驗證了全長ked樣蛋白與acamz的相互作用，結果為陽性。 -
3 fifty candidates proteins possible interacting with the nthk1 protein were screened from the tobacco cdna library through the yeast two - hybrid system
3利用酵母雙雜交技術從煙草的cdna表達文庫中篩到50個可能與nthk1的相互作用的蛋白基因。 -
Metallothionein - 3 ; yeast two - hybrid system ; interaction ; dimerization
金屬硫蛋白- 3酵母雙雜交系統相互作用二聚體 -
Yeast two hybrid system assay indicated that aes and tle1 interact by their homologous q domain
Dna的酵母雙雜交載體，通過酵母雙雜交系統分析證實aes與tlei分子通過氨基端的q結構域相互結合。 -
Detection of interactions among metallothioneins by yeast two - hybrid system detection of interactions among metallothioneins by yeast two - hybrid system
酵母雙雜交系統檢測金屬硫蛋白mt間的相互作用 -
We cloned full - length cdna of ca coding region via pcr amplification, but a negative result was obtained when the interaction of full - length ca and ga was tested in yeast two - hybrid system
通過rt - pcr克隆了ca編碼區全長cdna ，但是在雙雜交系統檢測全長ca與g蛋白的相互作用時，結果為陰性。 -
In order to study the upstream and downstream components in g protein and calmodulin signal pathways, a yeast two - hybrid system was used to screen interaction partners of these two proteins
本文利用酵母雙雜交系統分別篩選了與這兩種蛋白相互作用的蛋白質，以進一步研究g蛋白和cam信號轉導途徑中的其它上下游組分。 -
The yeast two - hybrid system is an effective method for the study of protein - protein interaction in recent years. it is widely used in the study of life science because it provides a genetic means of identifying proteins that physically interact in vivo
酵母雙雜交系統是近年來發展起來的一種研究蛋白質?蛋白質相互作用的有效方法，由於它檢測的是一種體內的相互作用，自這一系統建立以來，就被廣泛地應用於生命研究的各個領域。 -
The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination
為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用，將它們與nifa基因互換載體后共轉化酵母，仍然可以激活三個報告基因的表達，而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。 -
The results of gst - pulldown and yeast two - hybrid showed kyot2 interact with them respectively in and yeast and vitro. 3 using the yeast two - hybrid system, we isolated a novel protein, kbp ( kyot binding protein ). three types of cdnas were identified by rt - pcr and were designated as kbp1, kbp2, kbp3, respectively
通過rtpcr我們獲得了kbp全長的cdna ，並發現它存在三種不同的剪接體ep 、 kbpz和ep3 ，同時通過計算機比較還獲得了它的基因組全長，這三種cdna和基圇組序列已經在g bank上登錄于邱。 -
The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants. in this study, full length of phrip1 is amplified by pcr and ligated into pks plasmid, then the bait plasmid, peg202 - phrip1, is constructed. the inseret gene are sure to be translated into the right fusion protein through its sequence. in the yeast two - hybrid system, the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation. then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained. the two insert gene fragments are sequenced. one of them is plastocyanin, the other is putative photosystem i reaction center subunit ii precursor, both of them are the necessary components of photosynthetic chain
成膜素相關蛋白1 （ phrip1 ）是一個含608個氨基酸的蛋白質，它對于植物胞質分裂中細胞板的形成起到了十分重要的作用。研究phrip1的功能和機制，對在分子水平上闡明植物細胞板以及細胞壁形成的機理具有重大的生物學意義。在本實驗中，根據phrip1的序列設計引物對其進行pcr擴增，得到該基因后將其連接到了pks質粒上，並進一步構建成了誘餌質粒peg202 - phrip1 。 -
To identify the interaction between kyot2 and zo - 2 - i3, yeast two - hybrid system, purification of kyot2 protein and gst pull - down assay were performed in the experiments. after kyot2 and zo - 2i3 exchanged their vectors, yeast two - hybrid test revealed physical binding of the two proteins
本實驗通過酵母雙雜交及gstpull downas腳驗證了kyotz蛋白與zoz蛋白在體外的相互作用，並通過酵母雙雜交實驗初步確定其相互作用的位點。 -
Using yeast two - hybrid system with lim domain protein kyotl as a bait, we have isolated an alternatively spliced form of human tight junction protein 2 - - - - - - zo - 2 - i3. the tight junction ( or zonula occludens ) is the most apical junction between neighbouring epithelial cells or endothelial cells
53為深入研究kyoth在notch rbpjk途徑中的可能作用及其作用機制，本實驗室以kyoth為誘餌蛋白通過酵母雙雜交得到人類緊密連接蛋白zoe 。 -
Vimentin is a kind of type iiiinterfilament and has high structural homogenity with c - fos, c - jun and creb. vimentin also modulates translocation of some signal molecule. whether vimentin is a downstream event of plc - 1 pathway and whether vimentin is an interchange site between plc - 1 pathway and other signal pathways needs further confirmation by molecular biology analysis such as yeast two - hybrid system and western blot
實驗結果顯示：經過egf刺激24小時后，相對於野生型mef組，敲除型mef組的2一de圖譜中代表vimeniin的蛋白斑點消失，暗示缺失plc一y1基因可能直接或間接導致了vimentin的不表達， vimentin是否是plc一1通路的下游事件，以及是否是plc一yl通路與其他信號通路的交叉點，尚需結合其他方法進行深入研究。 -
Northern blot and rt - pcr analysis verified that the expression level of ldplcl was induced in germinating pollen, whereas ldplc2 expression level did not change during pollen germinating process. the interaction between heterotrimeric g proteins and plc was detected in a yeast two - hybrid system
Northern和rt - pcr分析，發現隨著花粉的萌發， ldplc2的轉錄活性提高，而ldplc1博士學位論文在萌發前後的轉錄水平沒有變化，推測至少ldplcz參與了對花粉萌發的調控。 -
Using yeast two - hybrid system honjo ' s group in kyoto university isolated a novel protein, kyot, which physically interacts with rbp - j. differential splicing gives rise to three transcripts of the kyot gene, kyotl, kyot2 and kyots. kyot2, but not kyotl negatively regulates transcription by association with rbp - j
為進一步探討rbp - j的功能，日本京都大學的honjo研究組利用酵母雙雜交系統篩選到一個可以與rbp - j相互作用的新蛋白kyot ， kyot蛋白有三種不同的剪切體： kyot1 ， kyot2和kyot3 ，其中kyot2可以與rbp - j相互作用抑制其轉錄活性。 -
In our work, different hbv pres gene fragments were amplified by pcr, cloned into pgbkt ? vector in yeast two - hybrid system 3, and then transformed ah 109 yeast cells. the results showed that these pres expression products were nontoxic to yeast cells, but all self - activated the yeast two - hybrid system
本研究將hbv前s區不同片斷進行擴增，克隆入酵母雙雜交系統3中的pgbkt7載體，轉化酵母細胞ah109 ，經檢測，證實了hbv前s區不同片斷對酵母細胞無毒性作用，但是都存在自激活作用。 -
The yeast two - hybrid system was established. in yeast two - hybrid system, mt - 3 interacts with mt - 3. a further two - hybrid test also indicated that mt - 3 weakly interacted with its isoform mt - 1. these primary results suggest that mt - 3 probably trend to homodimerize or heterodimerize in vivo
結果表明： mt - 3與mt - 3之間在酵母細胞內能發生相互作用，進一步用酵母雙雜交實驗還表明mt - 3與mt - 1間也存在弱相互作用。 -
This work provides a good foundation for the establishment of yeast two - hybrid system in our lab
本工作為我室酵母雙雜交方法的建立打下了一個良好的基礎。
分享友人