噬菌體基片 的英文怎麼說

中文拼音 [shìjūntǐjīpiān]
噬菌體基片 英文
base plate
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 體 : 體構詞成分。
  • 片 : 片構詞成分。
  1. High stability of ptr102 - derived marking plasmids indicated that novel vector systems based on ptr102 could be constructed for the study on molecular biology and ecology of m. huakuii. 1kb gfp cdna fragment amplified by pcr was cloned into e. coli expression plasmid pet - hc, which was under the control of rna polymerase gene promoter from phage t7 with its own translation initiation codon

    將pcr擴增的1kbgfpcdna片段克隆到大腸桿菌表達載體pet - 11c上，使gfpcdna在帶有lac操縱基因的t7噬菌體rna聚合酶基因啟動子的控制下、以自身的atg作為翻譯起始密碼進行翻譯。
  2. Thirteen putative epitopes showing characteristics of antigenic epitope were found from the analysis information. using pcr, the nucleotide acid fragments encoding these putative epitopes were amplified, then cloned into the expression vector miske. the positive recombinant phage displying the epitopes were found out by using pcr, sequencing and the determination of phage plaque titer

    運用goldenkey分子生物學軟體對prrsvbj - 4結構蛋白的抗原表位及其二級結構進行了分析和比較，從中篩選13段顯示表位特徵的氨基酸殘基序列，用pcr技術擴增相應的核苷酸片段，將其插入到噬菌體表達載體m13ke ，結果預測的13個表位可在噬菌體表面得以展示。
  3. A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf

    根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。
  4. The library consisted of 1. 3 x 106 clones with an average insert size of about 18kb. the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin. screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr, we got four positive clones

    3x10 『個重組噬菌體，插入片段大小約為18kb ，含插入片段的頻率為100隊以中亞濱蓉甜菜堿醛脫氫酶門adh ）基因近5 』端的約400hp片段為探針，篩選中亞濱蓉基因組文庫，得到了4個陽性克隆。
  5. Using pcr technology, a 2. 4kb dna fragment, part of tryptopanase operon, containing a promoter, a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e. coli k - 12, was cloned to pmd18 - t vector. the dna sequence is the same as which was published on science

    為了證明質粒上的基因能表達出有活性的色氨酸酶，將這個dna片段克隆到pet28c質粒的bamhi和hind位點上，使該片段受t7rna聚合酶的啟動子控制，然後轉化噬菌體de3的溶源菌bl21 （ de3 ） 。
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