培養液 的英文怎麼說

中文拼音 [péiyǎngyè]
培養液 英文
a culture fluid
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 液 : 名詞（液體） liquid; fluid; juice
  1. The process is repeated until a microbial culture acclimated to the specific industrial waste is developed.

    重復以上過程，直至微生物的培養液已達到馴化于特定的工業廢水為止。
  2. These cells were polygon and displayed morphologic characteristics of liver parenchymal cells at 24 hr of culture, such as a large round nucleus with a few nucleoli and many cytoplasmic granules, sometimes binucleate, hepatocyte was in direct contact with adjacent cells

    而肝細胞培養液組在低倍鏡下觀察，培養瓶的背景干猙，死細胞較少，貼壁細胞伸出多個偽足，呈多邊形。相鄰細胞連接成小片狀。
  3. We examined the cells began to adhere 12 hr after the cells inoculated. the pseudopod were determined at 48 hr of culture. these cells adhered displayed typical epithelial cells morphological characteristics : the hepatocytes had a rich cytoplasm and were sometimes binucleate

    用含10新生牛血清的rpmi1640培養液進行培養， 12h后細胞開始貼壁生長， 48h伸出偽足，呈現典型的上皮樣細胞的外形形態，胞漿內有空泡和脂滴，可以見到雙核細胞。
  4. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  5. Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52

    以細胞培養液m199 （含20 %的小牛血清）為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，採取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍，成活率為72 % ，第二組冷凍保存13天後解凍，成活率為520 / 0 。
  6. Subconfluent cultures of rat were maintained in dmem / 10 % fbs, 24hrs before neuronal induction, media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ), to initiate neuronal differentiation, the preinduction media were removed, and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media, 5hrs later, 40ul dmso was given to every hole containing 2ml each

    分別取第5代和第13代的mscs ，以8x10 cm 『濃度接種於六孔板中的蓋玻片上，制備細胞爬片，每孔加zinl培養液。達到80融合時，更換新鮮培養液，並在培養液中加人終濃度為lrnm的p一流基乙醇，誘導24小時， pbs洗滌，而後換成無血清的培養液。
  7. Poly ( lactic acid ) ( pla ) has been widely used as scaffolds for tissue engineering because of its good processability, good biocompatibility and suitable mechanical properties. but its catabolite would often induce erythrophlogosis. the preparation and properties of the pdlla / ha compound fiber and cell culture on the pdlla / ha unwoven meshes had research researched in this paper

    聚乳酸（ pla ）因其良好的生物相容性、生物可降解性以及良好的可塑性而被廣泛地應用於組織工程支架材料的研究，但是單一的聚乳酸長期在培養液或機體內因降解而易導致局部炎性反應。
  8. Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded

    方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。
  9. Effect of different seed - soaking means and culture fluids on seed germination capacity of platycodon grandi florum

    不同浸種方式和培養液對桔梗種子發芽率的影響
  10. Experimental study of the oriented differentiation of bone marrow derived mesenchymal stem cells into chondrogenic phenotype in a specific culture fluid

    大鼠骨髓間充質幹細胞在特定培養液條件下向軟骨細胞表型定向分化的實驗
  11. Es - d3 cells differentiated into ebs only when depleted of feeder cell layer by being cultured three generations in the presence of leukemia - inhibitory factor ( lif )

    結果表明： eso3細胞在含有lif的培養液中脫離飼養層培養3代以上才能形成ebs 。
  12. The numhers of day - 4 ebs per milliliter culture media are 44. 83 1. 22 ( n = 6 ) and 43. 17 1. 05 ( n = 6 ) respectively when cultured by hyclone fbs and homemade green season fbs

    在含進口hyclone胎牛血清或國產四季青胎牛血清的培養液中培養， es d3細胞形成的4debs數量分另為44
  13. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  14. Expanded bed adsorption ( eba ) is a novel bioseparation technique, which integrates clarification, concentration and initial purification into a single unit operation. it enables proteins to be recovered directly from unclarified cultivations of microorganisms or cells and homogenates of disrupted cells, without the need for prior removal of suspended solids. matrix is the principal " hardware " pillar supporting the successful application of eba

    擴張床吸附（ eba ）技術是一種新型的生化分離技術，它集成了固液分離、濃縮和初期純化於一步單元操作之中，可以直接從含有細胞和細胞碎片的發酵液或培養液中提取目標蛋白，而不必事先除去懸浮的固體顆粒。
  15. Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed

    摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此，人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。
  16. In vitro fertilization ivf

    培養液的質量在體外受精
  17. Koch perceived that it would be far better if one could solidify a well-tried liquid medium with some clear substance.

    郭霍設想，如果能用某種透明物質將經過多次試驗的培養液固化可能更好。
  18. This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan

    在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。
  19. Murine zygotes are more sensitive to commonly - used fertilization culture media

    小鼠合子對常用人體外受精培養液敏感性較高
  20. When the cells were then shifted to an enriched medium, the mutant cells proliferated.

    然後，當細胞移入一種滋養培養液時，突變型細胞增殖。
分享友人
分享到 Line

分享到臉書