堿基摻入 的英文怎麼說
中文拼音 [jiǎnjīchānrù]
堿基摻入
英文
base incorporation-
3. calcitonin gene - related peptide attenuates glutamate - induced inhibition of pulmonary surfactant lipid synthesis ( d cgrp could reverse the decrease of [ 3h ] - choline incorporation induced by glu with dose - dependence ; ( 2 ) cgrp could block the decrease of the content of cct a mrna induced by glu in lung tissue ; ( 3 } cgrp could reduce the impairment of the ultrastructure of at ii cells induced by glu ; ( 4 ) cgrp could reverse the increase of mda content and decrease of sod level induced by glu in cultured lung explants, respectively ; ? grp had no significant effect on nos activity and increase of no production induced by glu
降鈣素基因相關肽減輕谷氨酸所致肺表面活性物質脂質合成抑制的保護作用降鈣素基因相關肽grp ）可顯著減輕0所致肺組織h一膽堿摻入pc量的降低，並且呈劑量依賴保護效應； cgrp可逆轉gill所致cctqinrna含量的降低； cgrp可減輕o所致肺11上皮細胞超微結構的損傷； cgrp可逆轉o所致肺組織勻漿中mda含量增多、 sod水平降低的效應，並可逆轉q所致肺組織ldh釋放增多的效應； cg販對gill引起的nos活性和no含量的升高均沒有顯著性影響。Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。
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