寡糖基 的英文怎麼說
中文拼音 [guǎtángjī]
寡糖基
英文
oligosaccharyl-
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。[ result and discussion ] 1. combination between 2 - amac labeled oligochitosan and macrophage : 2 - amac - oligochitosan first bound the cytomembrane of macrophage, and then diffused in the whole cytoplasm, at last entered the nucleolus and diffused in the whole cell. fluorescence intensity increased with time
2 -氨基吖啶酮標記的殼寡糖與巨噬細胞的結合情況： 2 -氨基吖啶浙江大學碩士學位論文酮標記的殼寡糖先與巨噬細胞膜有結合，然後分佈於整個細胞質，最後進入細胞核，隨時間的進展而呈現了一個內在化過程。Label oligochitosan with amac : 400 u 1 of a 5mm aqueous solution of oligochitosan was lyophilized
255 。 oi 12氨基叮陡酮標記的殼寡糖繼續培養30分鐘。Ricin is a heterodimeric rip comprised of a and b chains joined through a disulfide bond
A鏈（ rta ）是活性鏈，分子量約32kd ，第十位殘基asn連接一條寡糖鏈。These interactions are primarily dependent upon the coordinated actions of ovarian progesterone and estrogen, moreover, many other factors, such as growth factors, cytokines, ecm, adhension molecules, oligosaccharides and proteases, regarded as local mediators, endometrium and embryo have also expressed some specific receptors, via intracellular signal transduction chains and express some key genes, making receptivity of the uterus and synchronized development of the embryo to the blastocyst stage
成功的植入是處于接受態的子宮內膜和具有侵入性的胚胎間的同步協調反應。植入過程受多種生長因子及其受體、細胞因子、粘附分子、蛋白水解酶、寡糖等的精細調控，通過胞內信號轉導及關鍵基因的表達使子宮內膜發生一系列復雜變化。Initial report on test on freshness retention of mango by chito - oligosaccharide - based polymer
保鮮劑殼寡糖基聚合物對芒果保鮮試驗初報3. observe the binding of oligochitosan labeled with 2 - amac with macrophages under confocal laser microscope and analysis the fluorescence intensity by flow cytometer using the cell quest software
在激光掃描共聚焦顯微鏡下觀察2 -氨基吖啶酮標記殼寡糖與巨噬細胞的結合，用流式細胞儀分析熒光強度。Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan, and be stimulated by ifn - r before oligochitosan added, then measured the changes of gene transcription and translation level of both il - 1 and imf - a, respectively by methods of relatively quantitive rt - pcr and elisa. first, rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan, then by the same method, confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating. because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone, so add ifn - r to microphages alone for 22 hours, then examined by rt - pcr, the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group
此外，由於ifn y單獨作用也可促進兩種細胞因子基因表達，故在巨噬細胞中加入ifn y單獨作用22h ，再經阿一pcr檢測，發現加ifn y的實驗組細胞的幾一lp和tnf a基因轉錄水平與空白對照組相比較無顯著性差異，可見，殼寡糖和ifn v對巨噬細胞il lp和tnf一口基因轉錄水平的影響在作用時間上無一致性，在殼寡糖作用最適時間時，僅受ifn y刺激的巨噬細胞il lp和tnf q基因轉錄己下降至刺激前水平，因此可以認為， ifn y的加入僅起到對巨噬細胞預刺激使之處于敏感狀態的作用，有利於增強殼寡糖對巨噬細胞的作用。There are still many questions remain unanswered, including the mechanisms that specify oligochitosan recognition, the participation of additional molecules in transmembrane signaling, and the repertoire of downstream pathways activated by oligochitosan. it was also observed that pdtc did not totally block no production, which gave us another evidence that there must be some other signal transduction pathways ( perhaps mapk, pkc, ca2 + dependent signaling ) involved in the activation, so further investigation is necessary to clarify molecular mechanism of oligochitosan inducing immunological en
Westemblot結果顯示100林歲ml劑量的殼寡糖作用於raw264 . 7細胞（ 4x105個細胞/ ml ） 4h ，胞核內p65的含量明顯增加， 6h時達到高峰， 10h時恢復到基礎表達量，這就提示在殼寡糖對巨噬細胞的激活過程中nf一kb活化狀態可以持續6個小時。Oligochitosan is not only water - soluble, non - toxic, biocompatible but also possesses versatile functional properties of chitin and its derivatives, such as polyelectrolite properties, the presence of reactive functional groups, gel - forming ability, high adsorption capacity, bacteriostatic, fungistatic and antitumour influence, and thus it has attracted more and more attention in the field of biology. previous investigations have shown that chitin and its derivatives have immunoenhancement activities
殼寡糖是由3 10個n -乙酰氨基葡萄糖殘基或氨基葡萄糖殘基通過- 1 ， 4 -糖苷鍵連接而成的寡糖，它具有良好的水溶性、組織兼容性，在生物體內易降解，同時也具有抑菌、抗腫瘤、調血脂、調節免疫及活化腸道雙歧桿菌等多種生理功能，因此已成為眾多領域的研究熱點。However, there are limitied documents about receptor signal transduction within uterine endometrium during implantation. the factors regulating endometrial receptivity remain poorly understood. the objective of this dessert is to investigate mapk ' s expression and location in differnt days of pregnancy, as more important, we try to elucidate the relation of mapk and the receptivity of endometrium
本課題組經研究還發現lewis ~ y寡糖在植入中呈階段特異性表達，可影響其他如lif 、 egf 、 fn （纖連蛋白）和mmps （基質金屬蛋白酶）等因子的表達，推測其可能作為一種信息分子經某種轉導途徑（例如mapk ）而發揮作用。The internalization of oligochitosan in macrophage is a time - course process. 2. initial experiments tested time, temperature, and concentration dependence of 2 - amac - oligochitosan uptake in macrophages. scatchard analysis of internalization of 2 - aminoacridone - oligochitosan in macrophages indicated its internalization is mediated by a specific receptor on macrophage membrane with ka of 2. 1 10 - 5m
2 . 2一氨基叮睫酮標記的殼寡糖與巨噬細胞的結合是一個依賴時間、溫度和濃度的過程， scatchard分析后發現它對巨噬細胞的作用是通過膜上特異性的受體（ kd為2 . 1x10一sm ）進行的。Producer of mono -, di - and trimethylamines, and derivatives including agrochemicals, feed additives, and water treatment chemicals. headquartered in belgium
-甲殼素殼聚糖殼聚寡糖d基葡萄糖鹽酸鹽d -氨基葡萄糖硫酸鉀鹽d氨基葡萄糖硫酸鈉鹽。The protein level of nf - kb in nucleus peaks at 6 h after stimulation and persists for at least 6 h. pdtc ( pyrrolidinedithiocarbamate ), an inhibitor of nf - kb, the effective concentration of it for nf - kb inhibition without any toxicity on cells is up to 100 umol / l, can significantly block no production induced by oligochitosan. although the study reported herein reveals that oligochitosan induces no production via nf - kb in macrophage, which is an important signaling mechanism of oligochitosan inducing immuno - enhancement of macrophage in vitro
加入100林mol / l劑量的nf一忍抑制劑毗咯烷二硫代氨基甲酸鹽（ pnte ）作用於rawz 「 . 7細胞（ 4火10 ，個細胞/ ml ） 90rnin ，然後再加入100林留而劑量的殼寡糖對巨噬細胞進行刺激，發現pdtc在各個時間點（ 6 、 12 、 18 、 24h ）都明顯抑制殼寡糖對巨噬細胞no的誘導作用。分享友人