In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋
芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、
芽的分化及生根的影響；然後在
得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
Barley-sugar makes children sweet-tempered.
麥
芽糖使小孩的脾氣變
得甜甜的。
Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )
獲
得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發
芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，
得到抗性葉片181片（基因槍93片，農桿菌88片） 。
Seed culture conditions of bacillus licheniformis ts - 01 were firstly investigated. the results showed that the optimal growth temperature was 40 c ; the suitable seed culture medium was beef extract soya peptone medium ; the optimal initial ph was 7. 5 ; the optimal seed culture time was 13h ~ 14h
首先對地衣
芽孢桿菌ts - 01的種子培養條件進行了研究，
得到的結果為：最適生長溫度為40 ；較適合的種子培養基為牛肉膏大豆蛋白腖培養基；最適初始ph值為7 . 5 ；種齡為13h 14h 。
The larks were silent, the young leaves of the early trees smitten and blackened.
百靈鳥沉默了，幼樹的嫩
芽也被打
得發黑。
Soup does very well without - maybe it s always pepper that makes people hot - tempered, she went on, very much pleased at having found out a new kind of rule, and vinegar that makes them sour - and camomile that makes them bitter - and - and barley - sugar and such things that make children sweet - tempered
也許正是胡椒弄
得人們脾氣暴躁。 」她對自己這個新發現非常高興，就繼續說： 「是醋弄
得人們酸溜溜的，黃菊把人們弄
得那麼澀，以及麥
芽糖這類東西把孩子的脾氣變
得那麼甜。
The cultural conditions such as temperature, fermentation period and the compound of medium are studied. the result of test show that suitable factors for both bacterium to grow and active substance to produce are 28, 200rpm and 72 hours. the bacterium is gotten through centrifuge with 8000rpm for 20min. then the bacteria is diluted and colophony named s - 8 is put into and used to absorbed active substance for 4 hours
對該菌株發酵條件的研究表明：該菌株用馬鈴薯葡萄糖液體培養基發酵培養， 28 ， 200rpm搖瓶中振蕩培養72h可獲
得高活性的發酵產物，用蘇雲金
芽孢桿菌hd - 1做指示菌，將發酵液稀釋40倍生測仍可形成明顯的抑菌圈。
When salt - coagulants : malt dextrin was 1 : 3, the quality of instant soybean curd was better
鹽類凝固劑：麥
芽糊精為1 : 3時，所
得的即食豆腐腦產品較好。
Illness related to c. difficile, a bacterium commonly found in hospitals, is becoming more widespread, more severe, and harder to treat, reports the april 2007 issue of harvard women ' s health watch
據《哈佛大學婦女健康觀察》 2007年4月刊報道，難辨梭狀
芽胞桿菌一種常見的院內菌，所引起的疾病正變
得越來越廣泛、嚴重且難以治療。
Foliation, bloom, and production became erratic.
萌
芽、開花和結果變
得很不正常。
It is well known that various kinds of biological substances such as growth factors, cytokines and adhesion molecules are closely related with the wound healing process. in particular, adhesion molecules play an important role in the promotion of inflammatory reaction, these factors stimulate the synthesis of extracellular matrix by local fibroblasts, generate new blood vessels, promote the granulation tissue fonnation, and enhance re - epithelialization nthat takes places by the migration of the kerati - nocytes from the edges of the wound toward the center
多種生物學介質如：生長因子、細胞因子及粘附分子等與皮膚損傷愈合過程密切相關，尤為值
得提出的是，粘附分子在炎癥的發生的起始過程中起著至關重要的作用，這些因子在細胞外基質的形成、血管的發生、肉
芽組織的生成及上皮的再形成方面等均具有重要作用。
6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草
芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，
得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
All the kingly countries of the east. her teaching was sown there first, where it sprouted and grew most strongly
她的教理最先在這些地方播種，這些種子發
芽生長
得最強壯。
Investigators have found that the knapweed ' s roots exude a toxin that builds up in the soil
研究者發現，這種矢車菊根部分泌的一種毒素會滲進土壤里，使
得其他植物的細胞大量死亡，並抑制萌
芽與生長。
The weeping-willow, the weeping-birch, and other trees of early and pendulous shoots, flourish in these favoured recesses.
這些幽靜去處
得天獨厚，垂柳，赤楊及其他抽枝發
芽較早的樹木長
得非常茂盛。
A larger number of budlike protuberances form and are pinched off to give microcells.
大量
芽狀突出物形成，並被脫出以獲
得微細胞。
Single malt scotch whiskies are distilled entirely from a fermented mash of malted barley
蘇格蘭純麥威士忌完全從發酵的大麥
芽漿中蒸餾獲
得。
The recombinant b. thuringiensis subsp. israelensis b - pmpx2 can produce a rhombus crystalline inclusion body during its sporulation sds - page analysis proved that cytlaa had overexpressed during the sporulation of the recombinant. it was found that b - pmpx2 strain remained stable toxicity, whatever during vegetative phase or sporulation phase, which was similar to the expression of mtxl gene in the strain of protease ( s ) - deficient b. sphaericus
同時，將來源於蘇雲金
芽孢桿菌以色列亞種（ b . thuringiensissubsp . israelensis ， b . t . i ）的cytlaa基因和p20伴侶蛋白基因克隆連接到質粒pmt9中，並轉化到蘇雲金
芽孢桿菌無晶體型中
得到重組轉化子b - pmpx2 ，電鏡觀察發現重組轉化子b - pmpx2形成一菱形晶體。
At the scent of water it will flourish and put forth sprigs like a plant
伯14 : 9及至
得了水氣、還要發
芽、又長枝條、像新栽的樹一樣。
No callus occurred from hairy roots on medium lacking 2, 4 - d. high concentration ( l. 0mg / 1 ) of 2, 4 - d could induce much more calli, but it obstructed the growth of calli and followed buds differentiation. the result of callus induction showed that the optimum medium for callus induction was ms + 0. 5mg / l 2, 4 - d + 0. 5mg / l 6 - ba and ms + 0
愈傷組織誘導最佳培養基為： ms 05mg l個d 05mg l ba ，愈傷組織生長的最佳培養基為ms 0ling 12 ， 4 d cling 16ea ；愈傷組織能在不含激素的培養基上自發地分化出不定芽，但分化頻率較低，而6ea對不定芽的分化作用較明顯，配合使用6習a和naa對芽的誘導及得芽率較好，不定芽誘導的最佳培養基為ms 10mgilnaa 05mg lia培養基。
