抗豬瘟血清 的英文怎麼說
中文拼音 [kàngzhūwēnxiěqīng]
抗豬瘟血清
英文
anti-swine-fever serum- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 豬 : 名詞[動物學] (哺乳動物) hog; pig; swine
- 瘟 : Ⅰ名詞(中醫指人或動物的急性傳染病) acute communicable diseasesⅡ形容詞(戲曲沉悶乏味) (of tradi...
- 血 : 血名詞(血液 多用於口語) blood:吐血 spit (up) blood; 血的教訓 a lesson paid for [written] in b...
- 清 : Ⅰ形容詞1 (純凈) unmixed; clear 2 (寂靜) quiet 3 (清楚) distinct; clarified 4 (一點不留) w...
- 豬瘟 : [獸醫學] swine fever; african swine fever; hog cholera
- 血清 : [免疫學] serum; blood serum血清病 serum sickness
-
After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum
使用分步透析法對變性的包涵體進行復性,將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原,初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months, the sensitivity and specificity of the diaphragm did not change, so it has good stability ; when 10 positive serum was detected 3 times, the result is reproducible, so the diaphragm has good reproducible. serums from experimental inoculated piglets was detected. the results showed that when the titer is l : 16, the pigs were infected with streptococcus suis ; and when 1 : 64, the pigs could survive after challege with streptococcus suis. all the results have shown that dot - ppa - elisa was a convenient, rapid, sensitive specific useful method for the detection of antibody
該法以硝酸纖維素膜為固相載體,包被膜載抗原製成的診斷膜片具有良好的特異性:不與仔豬副傷寒、豬巴氏桿菌病、豬大腸桿菌病、豬衣原體病、豬瘟、豬細小病毒病、豬偽狂犬病、豬布氏桿菌、豬丹毒陽性血清反應;膜片具有良好的靈敏性,陽性血清作2 ~ ( - 11 )稀釋亦能檢出;膜片具有良好的穩定性,在4至少能保存7個月,其靈敏性不變。The sows, which are not pregnant, are vaccinated by hcv vaccine of different dose and then detected antibody against hcv with ppa - elisa at different time
給空懷母豬接種不同劑量的豬瘟兔化弱毒疫苗,分別于不同時期用ppa - elisa檢測血清抗體。The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively
豬瘟病毒ez基因的原核表達: pcr擴增出當前豬瘟流行野毒株,中國豬瘟兔化弱毒( c株)兔脾組織毒ez基因的主要抗原區,將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達,經sds page檢測表明,重組質粒能表達ez基因主要區蛋白, westernblot檢測表明,誘導表達蛋白與豬瘟陽性血清發生特異性反應,表達量為35和38 ,可用於基因工程診斷抗原。分享友人