提別隆 的英文怎麼說

中文拼音 [dībiélōng]
提別隆 英文
tiburon
  • 提 : 提動詞（垂手拿著） carry （in one's hand with the arm down）
  • 別 : 別動詞[方言] （改變） change （sb. 's opinion）
  • 隆 : 隆Ⅰ形容詞1 （盛大） grand2 （興盛） prosperous; flourishing; thriving 3 （深厚; 程度深） deep; in...
  1. Based on the previous research on the portal crane administration, one standpoint put forward in this paper is idea that is on the foundation of reliability in this paper, two viewpoints take shape when reason is analyzed, that is fatigue break and appearing the flaw that will extend. what is the fatigue break ? ( this phenomenon is always happen in some place ), the fatigue is that board appears apophysis and concave when the board is pressed. so that the board ability of bearing the weight of load will descend

    本論文建立在已有的港口裝卸機械管理的研究基礎之上，進一步提出了以可靠性為基礎的港口裝卸機械的管理思想，已有的研究表明，港口機械設備破壞主要由於兩個原因，第一是穩定性問題，特別是構件的局部穩定性，所謂穩定性是指：在板的平面內，板受到平行於板面的壓力作用，使板發生隆起、內陷等凹凸不平的結果，從而使板受載能力減弱；第二是結構出現疲勞裂紋及其擴展，這是由於設備長時期運行，而造成設備某些部位出現裂紋並得以擴展，使設備承載能力下降。
  2. Constructing human colorectal cancer cell line with stably down - regulated grp94 ( 1 ) the plasmid prc / rsv - ribol that contains specific grp94 - targeting ribozyme and the control plasmid prc / rsv were miniprepared, respectively, cleaved by endoenzyme pvuii

    穩定下調grp94的人大腸癌細胞克隆株的構建（ 1 ）分別對含有特異性打靶grp94核酶的質粒prc rsv - ribo1和對照組質粒prc rsv進行小量提取、 pvu酶切鑒定。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. Guan xiaohong ( 1991 ) established a cell line of the monoclonal anti - idiotypic antibody ( anti - id ) np30 of schistosoma - 6 - japonicum, whose isotype was identified to be igm and which was testified to be internal image of gaa

    分別以日本血吸蟲單克隆抗獨特型抗體wbo的雜交瘤細胞株總基因組dna和其提取rna進行rticr合成的cdna第一鏈為模板，擴增v 。 、 v 。
  5. The donkey - adapted strain of eiav ( dv116 ), parental strain of eiav - dla, is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s. the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period. in this study, we constructed a full - length provirus dna clone of dv116 by pcr

    本實驗中將eiav驢強毒dv體外感染驢白細胞培養物，一定時間后收獲病毒（本文中簡稱dv116 ） ，提取eiav前病毒dna ，以pcr法分別擴增並克隆了包含全長基因的三段前病毒dna片段，以雙脫氧法測定了dv116病毒全基因序列共8236個核苷酸。
  6. Three trials were carried out by hualien, taoyuan and tainan district agricultural research and extension stations to evaluate the new sulfonylurea herbicide flazasulfuron for the control of weeds in non - crop land by post - emergence application

    摘要本研究于東部花蓮及西部桃園、臺南地區之不同氣候環境下，分別進行25 %伏速隆水分散性粒劑之田間篩選試驗，以探討防除非耕地雜草之有效施用方法，提供農民使用之依據。
  7. His current two major research projects, one in collaboration with professor yuan longping, father of hybrid rice, to develop a high yielding super - hybrid rice aiming at feeding tens of millions more chinese people, and another international collaborative project supported by the bill and melinda gates foundation to develop a nutrient - rich golden rice for humanitarian use, set good example of empathy

    辛教授現時領導的兩個主要研究計劃，分別為與雜交水稻之父袁隆平教授合作研究提升超級雜交水稻之產量，致力為數以千萬計的中國人提供溫飽，及由bill & melinda gates foundation資助的國際研究計劃，開發營養更豐富的黃金稻（ golden rice ） ，兩項計劃都充份體現辛教授關懷人類福祉。
  8. It is right to go together the industry to this gram that yuesheng breeze, yuesheng that designer that also seem to be to is very helpless, their speech : this kind of gram the s breeze ream they are proud of to again make them anxious about, the proud of is an own product to acquire the market with the approbation that go together, worry of is continuously for a long time hence, last in market positive development for having the yuesheng at considering yuesheng in the creation, style, disadvantage in go togethering the industry, let a hundred flowers blossom to is just spring. do not more lift under the watch elephant of the gram, the more is flow outing of appearance goods to counterfeit now, most the injure the consumer s benefits. the imitator is only meeting mimicry skin, copy not yuesheng creation is with the quality and to the vogue s sharp and full with confidence ability

    對同行業對悅盛的這股克隆之風，悅盛的設計師也顯得頗為無奈，他們坦言：這種克隆之風令他們自豪又令他們擔心，自豪的是自己的產品獲得市場和同行的認同，擔心的是長此以往，市場上只有悅盛在思考悅盛在創造，風格趨同，不利於同行業的良性發展，百花放才是春。更別提在克隆的表象下，更多的是假冒偽劣的樣子貨的涌現，最終損害到消費者的利益。模仿者只會模仿皮毛，模仿不了悅盛的創造性和品質以及對時尚的敏銳把握能力。
  9. Differential hybridization of human testis cdna micorarrays human testis cdna microarrays were constructed by spotted the pcr product amplified from human testis library. then membranes were hybridized using the probes of embryonic testis ( 6 months ) and adult testis

    人睪丸cdna微陣列雜交從人睪丸文庫中pcr擴增插入片段，將pcr產物點膜制備成cdna微陣列，然後提取胚胎和成人睪丸的mrna ，制備成探針，分別與微陣列進行雜交，獲取差異表達克隆。
  10. Fertilization is a highly programmed process by which two radically different cells, sperm and egg, unite to form a zygol it is mediated by complementary molecules present on the surface of the respective gametes. especially the proteins in the sperm acrom play an important role, but only a little can be extracted from sperm. now large amount of proteins can be obtained easily using biological technique

    受精是配子間相互作用最後融合成合子的過程，是由配子各自表面蛋白分子互補介導的，特別是存在於精子頂體的蛋白，但從精子中提取量少，用分子生物學手段大量克隆表達所需蛋白為受精機理的研究提供了方便。
  11. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  12. In order to furtherly study the petroleum accumulation law, this paper, based on the tectonic evolution, started from main hydrocarbon generation periods of the main resource rock, analysed petroleum generation, migration, accumulation, adjustment and dynamic accumulation process. according to area structural characteristic, bachu - markit was divided into the three sub - structure unit, that is, east part of bachu arch, west part of bachu arch and markit slope and established accumulation model of each part

    為了更深入的分析油氣聚集規律，本論文從主力烴源巖的主生油期出發，避開細枝末節，以區域構造演化為線索，分析油氣生成，運移，聚集，破壞調整，再次運聚成藏的動態過程。根據巴楚?麥蓋提地區的區域構造特點，將該區分為三個次級構造單元，即巴楚隆起西段、巴楚隆起東段、麥蓋提斜坡，分別建立了成藏模式。
  13. Because of the strong adaptability of csa, in this paper, based on csa, building blocks models constructing approach, mechanism of antibody ' s recognizing ball and fuzzy neural networks, a model identification approach for complex systems with uncertainty was presented. simulations are conducted

    本文採用克隆選擇演算法，結合抗體的模塊化構成方式、抗體識別球原理，以及模糊神經網路，針對具有不確定性的復雜系統，提出了一種基於模糊神經網路的免疫在線模型辨識方法，並進行了模擬驗證。
  14. George clooney is nominated for three oscars, as supporting actor in the thriller syriana and as director and co - writer of good night, and good luck, a film about the legendary newsman edward r

    喬治克隆尼被提名三項奧斯卡獎項，分別是驚悚片諜對諜中的男配角，以及晚安，祝好運中的導演兼劇本創作，這部片是新聞傳奇人物艾德華?莫若的故事。
  15. I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

    從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。
  16. The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach

    為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
  17. After the pbd i and pbd ii gene ligated with the expression vector pinpoint ? a - 3, the recombinated plasmid ppd - 1 and ppd - 2 was transformed into jm109 strains, 4 positive clones were screened by restriction enzyme analysis, dna sequencing showed that two out of 4 positive clones inserted sequence of the constructed plasmid, which was the same as that of pbd - i and pbd - ii gene respectively, and its reading frame was correct, thus its could be used to express fusion protein

    將pbd - 、 pbd -基因與表達載體pinpoint ~ （ tm ） xa - 3連結后獲得的重組質粒ppd - 1 、 ppd - 2轉化于大腸桿菌jm109中。經抽提質粒、酶切分析及pcr擴增，分別篩選到4個陽性克隆，將其中二個陽性克隆由測序分析，證實1個含pbd i基因片斷， 1個含pbd 11基因片斷，且閱讀框正確，可用於融合蛋白表達。
  18. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  19. This preliminary research focus on the tectonic uplift in late mesozoic - early cenozoic after the main collision oregeny to understand more and detail on the total process of tectonics evolvement in east dabie mountain and beihuaiyuan area. the research works that have been done are : apatite fission track dating, apatite thermal - evolvement patterns ; the fluid characteristics including fluid inclusions " microthermometer, the compositions, 5 d - 8 18o, and their geochemistry, thermodynamics parameters

    為更全面了解東大別山及北淮陽地區大地構造完整的演化，並為之提供研究實例積累，本研究主要從熱年代學及造山後流體特點角度對東大別山及北淮陽構造區碰撞造山後中生代未-新生代初構造隆升活動進行了初步探討。
  20. When providing and untilizing contemporarg enterprise ' s strategy management theory and the theory or knowledge related, the thesis not only pay special attention to the tide of world enterprises strategy management in the world, but also bases upon chinese social reality and the enterprise characteristic, takes the particularity of the state - owned enterprise into account simultaneously. it focuses attention on deepening reformation, especially on the reformation of the property rights of enterprises and the establishment of modern enterprise system when formulating the strategic target objective and enforceable tactics. while lays stress on humanly supervision and scientific mamagement when setting forward the management theory

    本文在介紹並運用當代企業戰略管理理論及相關理論知識的同時，特別注意既緊跟世界企業戰略管理理論潮流，又立足於中國社會現實和企業自身特點，充分考慮到國有企業的特殊性，在戰略目標及實施策略上，都把深化改革，特別是產權制度改革，建立現代企業制度作為重要內容；在管理理念上，既注重人本管理，又強調科學管理，致力於為隆昌工務器材廠這樣處于困難局面的國有中型企業尋找一條可持續發展道路，也為同類企業提供借鑒。
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