整合載體 的英文怎麼說

中文拼音 [zhěnggězǎitǐ]
整合載體 英文
integrating vector
  • 整 : Ⅰ形容詞1 （全部在內; 完整） whole; all; complete 2 （整齊） neat; tidy; orderly Ⅱ動詞1 （整理; 整...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 整合 : commensuration
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. With the help of the electronic computers, engineers employ it to figure out nearly all kinds of engineering problems ranging from astronavigation, construction, waterpower projects, ship - building and mechanical engineering etc. owing to the strong power of finite element method, one kind of software pack which based on the method is exploited and named algor feas to resolve problems on structure analysis for trusses and shells. this paper also employ algor feas software pack to analyze and compute the cableway towers, giving the accurate result on stress and displacement both in location and whole. finally, we also bring out some advice on structure improving

    在此分析過程中：首先將物理模型簡化為力學模型，主要是將塔架結構按空間桿單元簡化為空間桁架結構，目的是將力學模型變換為數學模型；這一過程主要是利用autocad2000建立塔架的計算模型；再者，利用algorfeas軟體包讀入autocad建立的塔架計算模型生成初始數據卡利用計算機進行編程進行風載計算，再將計算結論整合入algorfeas產生的初始數據卡中，生成計算數據卡；接著，利用algorfeas軟體包計算塔架在不同載荷情況下的應力圖，位移圖，並且求出在不同載荷條件下的最大位移和最大應力等；然後，對塔架的穩定性進行分析；最後；根據計算結果提出改進建議。
  2. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  3. Builds up communalized moral education in students " apartment park with the integration of social resources, develops the educational resource of community, expand the fields of moral education, creates the carriers of education activity, explores the effective ways of moral education, and gives effective play to moral education

    （ 5 ）整合社會資源，開展園區德育社區化建設。綜合開發社區教育資源，拓展德育領地，創造活動載體，擴大德育途徑，發揮德育的整體效能。 （ 6 ）運用網路科技，開展園區德育網路化建設。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  6. The flow diagram of a lpg bottle paint - drying heating system using heat carrier as heat - medium, which has similar properties as other heat - carrier heating systems, has been improved, which has good effect in avoiding oil - spraying, shortening dehydration time and power - fail protecting etc. after making a series of analysis, calculational methods of expansion tank dimension have been deduced, which takes the moisture boiling - off of heat carrier into account and accords with real conditions

    對與其它熱載體加熱系統具有共性的鋼瓶熱載體加熱系統的流程圖進行了改進和完善，在防止噴油、縮短脫水時間和停電保護等方面取得了良好效果。通過分析，整理出了熱載體加熱系統膨脹器容積的計算方法，該方法考慮了熱載體所含水分汽化而帶來的影響，比原有的計算方法更加符合實際。
  7. The artificial antibacterial peptide - ceme gene was designed according to the codon with the highest frequency in pichia pastoris, then the ceme gene was integrated into the chromosome of pichia pastoris strain - gsl 15 by electroporation

    我們根據人工設計的新型抗菌肽ceme序列設計ceme基因，重組到酵母胞內表達型的整合載體phild2中，通過電轉化作用將ceme基因整合至酵母宿主gsll5染色體上。
  8. Css is the one of the colonization factor antigens which is a protective antigen can cause immune reaction by the means taking orally. in this study, carrot was separately transformed with agrobacterium tumefacience strain lba4404 that contains ctb or ctb - cs3 fused gene in order to get oral diarrhea vaccine. this is a potent strategy to produce etec oral vaccine

    本研究期望通過農桿菌轉化系統將ctb （霍亂毒素b亞基，可作為佐劑和載體）基因和ctb - cs3融合基因分別轉入胡蘿卜植株，使ctb和ctb ? cs3融合基因穩定整合到胡蘿卜基因組內，希望以此獲得以胡蘿卜為受體的etec口服疫苗，使服食者在進食的同時就可獲得腹瀉免疫。
  9. Then introduce the present situation service of cosco - logistics ltd : appliance logistics, car logistics, electric power logistic, petrifaction logistics, exhibit logistics and retail logistics. on the base of swot analysis of cosco - logistics ltd, chapter four gives suggestion to logistics service innovation of cosco - logistics ltd : developing electronic commerce, integrated marketing, customization logistics and 4pl ? increment service. then give some supports strategy for logistics service innovation of cosco - logistics ltd

    第四章通過對中遠物流的swot分析，結合物流服務發展的趨勢，提出了中遠物流企業開展服務創新應該以電子商務為載體、以一體化營銷為基礎、以客戶訂制和第四方物流為發展方向、以增值服務為中遠物流的核心競爭力，在此基礎上，提出了開展物流創新所需要的物流設備的現代化、物流資源整合、物流信息化、物流人才的培養等方面的支持政策。
  10. Crystal zirconium phosphate - phosphonate has the stability and regulity of interlayer floor of inorganic a - zirconium phosphate, they still have the designability of the organic group in the interlayer and adjustment of the ratio of organic and inorganic phosphorus acid, they are a kind of potential smart material, we can process molecule design and optimize filtration according to the requirement of goal reaction, we can prepare special ion - exchange, catalyst, catalyst supporters nonlinear optics compound, solid state proton conductivity and the intercalation complex precusor by introducing different active organic group or active center into the choice and stable framework of inorganic a - zirconium phosphate

    有機?無機晶態混合磷酸鋯具有無機磷酸鋯的層板穩定性和規整性，同時具有層間有機基團的可設計性和有機無機磷酸混合配比的可調節性的特點，是一類很有潛力的靈巧材料，並且可以根據目標反應的要求進行分子設計和優化篩選，在無機磷酸鋯優良穩定的骨架上引入不同的活性基團或活性中心，可以制備出性能獨特的離子篩、催化劑、催化劑載體、非線性光學物質、固態質子導體和制備插層復合物的前驅物。
  11. The curves of the consolidation settlement in soft clay base of flood land are gained, which compare the effect of sand drain. the settlement - time curves in different period are also gained, which increase the height of embankment to 8m, based on the test data of hang - yong highway. furthermore, the consolidation settlement characters of these entity engineering are analyzed, and, the variation rules of the rate of grade and degree of the curves in different period in the whole section, the maximal settlement and the liner regression relations between the rate of grade and degree are calculated

    本文利用大變形固結有限元分析程序（ lscfea ）中的小變形平面固結分析單元（ sse2 ） ，結合實體工程項目進行了地基固結沉降的有限元計算，分別得出河灘相軟土地基在考慮砂井和未考慮砂井作用下的固結沉降曲線及杭甬高速公路試驗段在高堆載情況下的固結沉降曲線；並對以上曲線特徵進行了分析，得出各時間段曲線斜率、角度在整個斷面的變化規律，以及沉降最大值與斜率、角度變化之間的線性回歸關系。
  12. Meanwhile, the male mice deficient for germ - cell cyritestin are infertile. therefore, both spl7 and cyritestin are important molecules in the process of fertilization. nevertheless, people can not get fully satisfied the results in some contraceptive researches for their limited effects respectively

    其n端含有去整合素結構域，然而多數實驗結果表明單一應用這些分子所獲得的免疫性避孕效應非常有限，從而提出今後的研究方向是更具抗原性或良好運載體系的新型多價疫苗。
  13. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  14. Based on the analysis of commercial banks " current concept about fund management, this paper brings forward that fund management is the main - string in its operation, and that the concept of fund management, including security and profitability, is extended from current " forrying fund " to the management of the fund cost and fund risk the paper comprehensively discusses the principle of fund management, the management of fund costs, the tactics of management about fund liquidity, the measures of management about fund risks and how to solve the problems on interest risk in the period of frequent interest fluctuation. the paper puts forward ideas on how to improve the fund management. the security, liquidity and profitability of the fund, which are both contradictory and integrated with one another, are internal factors of fund management. fund liquidity is traditional core question. commercial banks face with a number of risks of witch credit risk is the greatest one because our country has adjusted interest rates 8 times since 1996, which covered a period of frequent interest fluctuation

    商業銀行面對許多風險，但最大風險是信用風險。由於我國自1996年以來已連續調整了8次利率，近幾年是利率波動頻繁時期，研究利率的敏感性問題顯得特別重要。要改善資金管理，提高商業銀行的經營水平，就要建立資金管理是商業銀行經營主線的理念，對資金要統一規劃和管理；要改革銀行的體制，建立現代企業制度和法人治理結構，在體制上為資金管理提供有利的運行平臺；增加改善資金流動性管理所需的貨幣政策工具，擴大資金調控手段；打破貨幣市場的僵化局面，為資金管理創造有利的宏觀環境；續續優化負債結構和負債載體設計；增強資金信用風險規避和化解的措施；通過銀行資源整合，努力尋找資金的安全投放渠道，最終完成經營模式由傳統型向現代型的轉變。
  15. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. orit from e. coli plasmid rk2 was also incoporated into the vector, therefore, pij903 derivatives can be mobilized from e. coli into streptomyces sp. fr - 008 by either bi - parental conjugation or by conjugation between streptomyces

    為了在克隆完整基因簇時避免這兩方面的影響並能隨機地獲得dna大片段，我們將第二代yac載體pjs97 - pjs98改造成為適合於在白林泉鏈黴菌基因組中dna大片段的基因置換與克隆鏈黴菌中分析被克隆大片段功能的基因置換載體。
  16. Firstly, gravel piles in soil were laid for compacting and consolidating soil, then gravel composite foundation was treated with dynamic compaction in order to form three - level soil that is gravel composite soil and high replacement ratio composite foundation and dense composite foundation

    該法先在填土層中設置好碎石樁體，利用擠密和排水固結的作用使其得到初步加固，然後對荷載影響深度范圍內的復合土體進行低能強夯處理，迫使樁體碎石沿徑向擴散，形成上部為密實的碎石二合土層、中部為擴徑后高置換率復合地基、下部為整體夯密復合地基的三層結構。
  17. Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce

    （ 3 ）細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣，細胞核轉化獲得了生長良好的抗性萵苣植株，經pcr 、 pcr - southern 、 southern斑點雜交分析證實， adw基因已整合到萵苣基因組中。
  18. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  19. The key subsystems that influence the cultivation and promotion of core competence are in systems of technology and r & d, human resource, organization and culture. based on the strategic priority of core competence, the paper builds a synthesis analysis framework which focous on the pmi of human resource, culture, organization system, technology and r & d. the analysis framework of pmi is described as below : firstly, the main task of core competence based human resource integration is to keep the key employee stabilization

    從上述四個視角來看，企業核心能力內化于企業的整個組織體系中，主要體現為關鍵的技術和技能、企業內部的獨特的知識集合、組織和管理機制、獨特的企業文化等幾個方面，其載體是個人和組織兩個層次，因而影響核心能力的培育和提升的企業子系統關鍵在於技術和研發子系統、人力資源子系統、組織子系統和企業文化子系統之中，因此，本文基於核心能力的戰略優先級，選取以人力資源整合、文化整合、組織機制整合、技術和研發整合四個子系統建立一個比較完整的綜合性的分析框架，這也是通過並購來培育和提升企業核心能力重點關注的領域。
  20. Pcr products were inserted into pbluescriptsk using psti and xhoi. the element bearing resistance to streptomycin ( sm ) and spectinomycin ( sp ) was excised from php45 and inserted into gene coding region. the disrupted gene was isolated as a pstl and xhol fragment and cloned into prl271

    首先將pcr擴增片段（ 1 . 5kb ）利用pst和xho插入常規克隆載體pbluescriptsk ，利用基因內部合適的酶切位點插入sp sm抗性片段，再利用pst和xho位點將已插入抗性基因的pcr片段，轉入整合載體prl271中獲得體外基因中斷，最後利用三親本雜交獲得體內突變。
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