整合酶 的英文怎麼說

中文拼音 [zhěnggě]
整合酶 英文
integrase
  • 整 : Ⅰ形容詞1 （全部在內; 完整） whole; all; complete 2 （整齊） neat; tidy; orderly Ⅱ動詞1 （整理; 整...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 整合 : commensuration
  1. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  2. Integrin linked kinase, ilk

    整合蛋白相連激酶
  3. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  4. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  5. Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch

    利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。
  6. The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn

    用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。
  7. Integrase is one of the three enzymes of the irus that is required for iral replication

    整合酶是病毒復制所需要的三種酶之一。
  8. It is useful for new drug design. ( 3 ) the docking approach was applied to predicate the binding of hiv - 1 integrase and its inhibitor aurintricaboxylic

    （ 3 ）運用分子對接方法對hiv - 1整合酶與其抑制劑金精三羧酸的結合過程進行了研究和預測。
  9. Integron consists of an integrase gene ( inti ), a integrase specific recombination site ( atti ), and gene cassettes with varied number

    整合子由整合酶（ integrase ）基因（ intl ） 、整合酶特異性重組點atti和數目不定的基因盒（ genecassette ）組成。
  10. The best bet at the moment is probably that the phage integrase method can be used by getting a better hold of how its site - specificity works and therefore being able to design changes to it rather than using the very weak technique of in vitro evolution - - but this will need a lot of hard work

    目前大可一賭的是：嗜菌體整合酶方法也許可以利用：較好地拿捏位點特異性使之起作用，而因此能設計改變嗜菌體整合酶，而不是利用體外進化的很不可靠的技術?但這需要做大量艱苦的工作。
  11. A aada2 cassette, encoding aminoglycoside adenylyltransferase, was found in 2 isolates of shigella sonnei, and a two cassettes array of dfra1, encoding dihydrofolate reductase, and aadala in one isolate of shigella sonnei, and another two cassettes array of dfra1 7 and aada5 in two isolates of shigella sonnei and shigella flexneri y - variant each, and a dfrv cassette in one isolate of shigella flexneri 6, respectively

    對其可變區測序分析，其中2株宋內志賀菌i類整合子均攜帶1個基因盒，為氨基糖昔腺營酞基轉移酶（ aminoglycosideadenylyltransferase ） az基因（ aa朔2 ） ，編碼對鏈黴素的抗性。
  12. It is divided to extracellular and intracellular part by transmembrane domain. there are 13 n - glycosylation sites, 20 protein kinase c phosphorylation sites, 28 casein kinase ii phosphorylation sites, 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein. an integrin recognition sequences rod lies in intracellular part of bt - r3 protein

    跨膜區域（ tmd ）將它分為胞內和胞外兩個部分，它的胞外有13個潛在的糖基化位點， 20個蛋白激酶c的磷酸化位點， 28個酪蛋白激酶的磷酸化位點， 4個酪氨酸酶的磷酸化位點， 15個豆蔻（十四烷基）酰化位點；它的胞內有1個整合蛋白（ integrin ）識別位點。
  13. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  14. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  15. The identified proteins are involved in a variety of cellular process including several zinc finger proteins relevant to transcription regulation, such as zinc finger 198, 263, 14, 224, zf6, novel protein similar to transcriptional represser ctcf, and kruppel - like zinc finger protein ; two members of the adams ( a disintegrin and metalloprotease domain ) family ; two members of integrin family ; several proteins involved in the signal transduction, cell - cycle control, chromatin remodeling and transcription repression ; and also some proteins of cell skeleton and some with unknown functions

    鑒定出的蛋白包括多個與轉錄調控相關的鋅指蛋白，如鋅指蛋白198 、 263 、 14 、 224 、 zf6 、轉錄抑制因子ctcf樣蛋白、幻肚pple樣鋅指蛋白等:兩個含金屬蛋白酶結構域和整合素結合結構域的家族成員adam28和adam17 ;兩個整合素家族成員pz整合素和含十個egf樣結構域的整合素（ tied ） ;與細胞信號轉導通路有關的蛋白;與細胞周期調控有關的蛋白;與染色體重塑和基因轉錄抑制有關的蛋白;細胞骨架蛋白以及其他功能未明的蛋白等。
  16. The recombination expression vector, ppic9 - e2, was linearized by sal i and electroporated into p. pastoris gs115. recombinant p. pastoris gs115, designated gs115 - ppic9 - e2, was identified by pcr and the product of the pcr was analyzed by sequencing before its expression for csfv e2 protein

    重組表達載體ppic9 - e2經sal酶切線性化后，電轉化整合到畢赤酵母gs115基因組上，經pcr鑒定和pcr產物測序分析，陽性轉化子命名為gs115 - ppic9 - e2 。
  17. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  18. It " s the first cdna code ( 6 - 4 ) photolyase found in low orgnism ( alga ), and this study is important for the reseach of 6 - 4 photolyase. the methods and analysis as bellows : 1. this est was analyzed by method of blast, and the conclusion suggests that the sequence probably be a partial cdna that can code one of protein family of photolyase / blue light photoreceptor

    利用3 』 race法擴增此cdna序列的3 』端部分，所得片段長度為2575bp ，含一個完整的開放讀框，長1800bp ，可編碼600個氨基酸；利用生物信息學法對此擴增序列進行分析，包括同源性分析、序列的讀框分析、蛋白質的保守性分析以及該蛋白的進化關系分析，預測出此序列編碼了d . salina的（ 6 - 4 ）光裂合酶。
  19. By this means, modifying the tnpi gene can get the different vectors with different resolution speed. 3

    若在解離載體中引入整合酶基因，在大腸桿菌中無法篩選到轉化子。
  20. Effect of integratase tnpi on resolution reaction of resolution vector the integratase tnpi has great effect on the speed and time of resolution reaction

    整合酶對載體解離特性的影響整合酶對解離載體的解離速率和時間影響很大。
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