整酶 的英文怎麼說

中文拼音 [zhěng]
整酶 英文
complete enzyme
  • 整 : Ⅰ形容詞1 （全部在內; 完整） whole; all; complete 2 （整齊） neat; tidy; orderly Ⅱ動詞1 （整理; 整...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  1. Biotransformation in organic solvent is an attractived field in nowadays. compared with isolated enzyme whole cell is not used broadly as biocatalyst. in this research the cells of baker ' s yeast is adopted to mediated a model reaction in organic solvent, in which geraniol is converted to citronellol reductively

    有機相生物轉化是當前生物技術中一個具有理論研究意義和應用價值的領域，目前該領域的研究大多集中在利用分離的酶進行生物催化，利用微生物完整細胞進行的研究比較少。
  2. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  3. The global regulator csra of e. coli is a specific mrna - binding protein. csra negatively regulates several metabolic pathways that are induced post - exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism ; positively controls gene expression within the glycolytic pathway ; and also csra modulates the levels of enzymes that participate directly in pep metabolism

    Csra是整體調控網路的調控基因，可負調控指數生長後期誘導的一些代謝途徑，包括糖原的生物合成、糖原的分解代謝、糖異生，而對糖酵解的一些重要基因的表達則執行正調控功能， csra也調控直接參與pep代謝的三個酶的活性水平。
  4. Softening finishing of natural color - cotton fabrics with cellulase and pectolase

    天然彩色棉織物的生物酶柔軟整理
  5. To cultivate students ' ability, author initiates tentative teaching reform in the aspects of teaching content adjustment, teaching method improvement and diversification of test mode exemplified by " food enzymology "

    摘要圍繞強化學生能力的培養， 《食品酶學》課程應當從教學內容的調整、教學方法的改進及考試方法的多樣化等方面對該課程的教學進行改革，以期達到更好的教學效果。
  6. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  7. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  8. When a catalytically active enzyme forms a complex with a cofactor a holoenzyme is produced

    酶蛋白和輔助因子兩者結合成完整分子時，形成具有活力的全酶。
  9. The extracted marrows ganoderma lucidum and semen zizyphi spinosae contained in threesss can regulate secretion of hydrocortisone in human body and reduce excitation of human body during nighttime ; in addition, ganoderma - lucidum spores and semen zizyphi jointly stimulate secretion of sleeping - inducting peptide in body. improve rate of " high efficient sleeping quality and immunity. recover and strengthen mental strength, mental strength and vigor. through sleeping. the psychlogy and physiology return to the youth level

    「索萊爾」多元明膠囊中所含的靈芝和酸棗仁提取精華，能調節人體內皮質醇的分泌，降低人體在夜間的興奮度；此外靈芝孢子粉和酸棗仁共同作用，能夠在體內刺激「睡眠肽」和格里酶「的分泌，有效促進「高能睡眠」率，延長「高能睡眠」時間，從而達到全面改善睡眠質量，提高免疫力，使體力、腦力、精力得到迅速恢復和加強，通過睡眠將生理及心理狀態調整到青春期水平。
  10. Integrin linked kinase, ilk

    整合蛋白相連激酶
  11. Some, including an esterase, may be on the outside of intact lysosomes.

    包括酯酶的一些，可以位於完整溶酶體的外表面。
  12. The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide

    核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。
  13. Phagocytosed tracer particles can permeate the whole lysosmal system.

    被吞噬的標記顆粒能通過整個溶酶體系統
  14. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  15. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  16. Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch

    利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。
  17. It was adopted that the adequate controls on fermented grains proportioning, starch content, moisture content, acidity, pit - stepping, and the use of saccharifying enzyme and dry yeast to ensure good fermentation in pits and further to realize safe trans - production of luzhou - flavor tequ liquor in summer

    摘要濃香型特曲酒夏季壓排、轉排生產要合理調整工藝配方，從糧醅比、澱粉含量、水分、酸度、踩窖以及糖化酶、乾酵母的使用等多個方面進行合理控制，保證窖池的良好發酵。
  18. Expression of this truncated gene in plant was expected to give information about expression in plant of high g + c content genes but no antifungal activity was expected in this stage

    由於載體中2 . 7kb的pks基因編碼了兩種酶活性的結構域而不是完整的型pks模塊，因此我們目前並不期望這個截短的pks基因在植物能產生抗真菌活性。
  19. The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn

    用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。
  20. The activities of nr increased fistly and then decreased after uv - b treatment. the gdh activities of treated plants were lower than those of control plants during the whole experiment. enhanced uv - b radiation reduced the contents of no3 - - n in spirodela polyhiza

    Uv - b輻射處理后硝酸還原酶（ nr ）活性呈先升后降的變化趨勢，谷氨酸脫氫酶（ gdh ）活性在整個處理期間均低於對照； uv - b輻射導致硝態氮含量降低。
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