末端基因 的英文怎麼說

中文拼音 [mòduānjīyīn]
末端基因 英文
terminal gene
  • 末 : Ⅰ名詞1 （東西的梢;盡頭） tip; terminal; end 2 （非根本、非重要的事物） nonessentials; minor detai...
  • 端 : Ⅰ名詞1 （東西的頭） end; extremity 2 （事情的開頭） beginning 3 （門類; 方面） item; point 4 （原...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 末端 : end; terminus; terminatio; terminal; tail end; extremity末端棒 terminal bar; 末端朝上 endways
  1. Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization

    用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒（ cmv ）的亞基因組啟動子區互補dna序列為標記探針，對自然感病的天南星科植物進行rna斑點雜交，並結合雙鏈rna分析、病毒提純和形態學觀察，對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。
  2. Methods and applications of inserting a stop codon into a cloned gene with pcr

    在已克隆基因末端添加終止密碼的方法與應用
  3. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  4. J mol biol., 1992, 224 : 53 - 63. 26 abe h, aiba h. differential contributions of two elements of rho - independent terminator to transcription termination and mrna stabilization. biochimie, 1996, 78 : 1035 - 1042

    通過計算，我們預測到266個不依賴終止子，其中包括232個蛋白編碼基因， 12個trna基因和3個rrna基因，約17 %不依賴終止子位於操縱子的末端。
  5. A conservative motif, recognized by proteinases of potato virus y, was inserted between nib and ppiv, which will release functional ppiv from the fused protein after infection by potato virus y. then, plant expression vector pnpa was constructed by ligating the fusing gene and pbi121, which is knocked out gus gene

    以馬鈴薯y病毒的復制酶（ nib ）基因為模板，通過聚合酶鏈式反應獲得nib基因，並在nib基因末端保留了在病毒基因組中nib與外殼蛋白（ cp ）基因相銜接的保守序列。
  6. Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence

    用rt - pcr技術，以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物，從蠶豆葉片中還擴增到了3個618bp （ 618 - 1 、 618 - 2和618 - 3 ）的cdna片段；用race技術，以618 - 1序列設計的一對基因特異性引物，克隆到了還缺少5 』末端的cdna片段vfcpk2 。
  7. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
  8. Situated at the tip of the malay peninsula and at the crossroads of the pacific and indian oceans, singapore ' s geographical location meant that its role in trade and communication between east and west cannot be challenged

    基於歷史因素，新加坡因位於馬來半島末端，太平洋印度洋水域的交匯處，這決定了新加坡在東西方通商交流中不可替代的地位。
  9. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  10. The best time to collect the materials for cloning is discussed in this paper, which is from the end of may to the beginning of june each year. it means there is only a week for the materials to transit from apex dividing tissue to flower inflorescence, but still a little change with temperature every year

    實驗探討了克隆成花計時基因最佳的取材時間是，每年的5月末到6月初，頂端分生組織向花原基轉變時的一個星期內集中進行，具體時間隨每年溫度變化做適當調整。
  11. To elucidate further the presense and function of integrin - like protein, we try to clone 3 " terminus seqence of integrin - like gene from germinated pollen of lily by 3 " race using degenerate primers corresponding to the conserved cytoplasmic regions of a and 3 subunits

    而要進一步從分子水平上證實類整合素的存在以及研究它的功能，必須克隆其基因。根據動物整合素、亞基的胞質域保守序列設計簡並引物，利用3 race的方法擴增、亞基的3末端序列。
  12. A pair of primers were designed according to the published sequence of gnrh2 ' s gene mrna and transporter gene with oligo version 4. 1 softwarre, they were annealed by their 3 ' terminal brief complementary base sequence to form small segment double chain, therery, they serve mutually as template and primer, and their extension were carried out to synthesize 90 bp " gnrh / trs

    本研究根據genbank中已發表的人gnrh2基因mrna序列以及轉運肽（ transporter ， trs ）基因核苷酸序列，藉助oligo4 . 1設計了一對寡核苷酸引物，以引物3末端的短互補序列退火形成小段雙鏈，從而互為模板和引物，通過引導合成長達90bp的gnrh trs序列gnrh trs 。
  13. For safety reasons, an overrun tunnel of sufficient length is required at all terminal stations to ensure trains failing to stop at the design position, as a result of human error or defective equipment, would not collide with the tunnel end

    基於安全理由，所有終點站均須設有足夠長度的掉頭隧道，確保列車因人為錯誤或機件故障而停站位置失準時，不會與隧道末端相撞。
  14. The full - length sequence, the 3 ' - deletion fragment, the sequence encoding the mature protein and the sequence encoding the conservative domain, were cloned using synthesized primers. recombinant expression vectors were constructed through directional cloning and then host e. coli were transformed by the vectors

    設計特異引物克隆得到毒蛋白基因的4個片段，即基因全長、末端缺失片段、編碼成熟肽的片段及編碼活性區域的片段。
  15. The former expresses recombinant proteins with a 6xhis tag at n - terminal. the fore mentioned four fragments were all used for expression in this system and the mature protein and the conservative domain were effectively expressed while the expression of the other two was unobvious

    前者表達n ?末端加6 his標記的融合蛋白，克隆到的4個基因片段均進行了表達，其中成熟肽和活性區域得到了大量表達，蛋白全長和末端缺失片段表達不明顯。
  16. The two fragments were ligated to a whole fusion peptide gene, next was ligated with two adapters which have the same cohesive end ( atg / tac ), respectively

    首先將其連接成融合肽的完整基因，然後分別與含有相同粘性末端（ atg / tac ）的ecor前接頭和sal後接頭連接。
  17. The end of may brought a vivid illustration of that promise - - and a surge of unease at the prospect of releasing genetically engineered animals into the wild

    5月末這個迷人的前景已經初見端倪，當然對于把轉基因動物釋放到大自然中去的結果人們還是頗感不安的。
  18. Of seven sweet - taste proteins, brazzein has smallest molecular mass, simply molecular structure and is most heat - stable. in order to make use of brazzein, we have studying on the expression of brazzein gene in e. coli and plants : lettuce and tobacco. the results obtained are summarized below

    為了更好的利用甜蛋白，探討brazzein基因在原核生物和植物中的表達，本論文進行了一些研究，結果如下： 1以合成的brazzein基因為模板，通過引物設計引入突變位點，在基因5 』末端引入ala的密碼子替代原有序列中編碼pglu的密碼子。
  19. 4. the seguence of cloned endostatin gene was confirmed with the dideoxy chain - termination method and is consistent with reported sequence of mouse endostatin

    4 .以雙脫氧末端終止法對重組質粒pbv220一endostatin進行測序，結果表明與己知小鼠endostatin基因序列一致。
  20. So, we subcloned emt - 1 gene into the prokaryotic fusion expression vector prsetb and generated a 24ku protein

    為此將emt l氨基末端基因克隆入融合表達載體prsetb中，轉化大腸桿菌bl 21 ，經iptg誘導，表達his6 emt l融合蛋白，表達產物分子量約為24ku 。
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