果糖已糖激酶 的英文怎麼說
中文拼音 [guǒtángyǐtángjī]
果糖已糖激酶
英文
fructohexokinase- 果 : Ⅰ名詞1 (果子) fruit 2 (事情的結局; 結果) result ; consequence 3 (姓氏) a surname Ⅱ動詞(吃...
- 糖 : Ⅰ名詞1 [化學] (碳水化合物) sugar 2 (食糖的統稱) sugar 3 (糖果) sweets; candy; sweety Ⅱ形容...
- 激 : Ⅰ動詞1 (水因受到阻礙或震蕩而向上涌) swash; surge; dash 2 (冷水突然刺激身體使得病) fall ill fr...
- 酶 : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
- 果糖 : fructopyranose; fructose; laevulose; levulose; levulosum果糖分解 fructolysis
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Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method
首先,以成體牛蛙的腦垂體總rna為模板,進行rt ? pcr擴增得到約660bp的特異性pcr產物帶,切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收,將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后,進行菌落pcr 、質粒酶切鑒定,篩選出陽性菌株,測序結果經blast分析,與已報道的牛蛙生長激素基因高度同源,證實此陽性克隆為牛蛙生長激素基因轉化子,命名為pbfgh 。
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