染色粒帶 的英文怎麼說
中文拼音 [rǎnshǎilìdài]
染色粒帶
英文
chromomere band-
The powder was then applied to a silica gel column chromatography charged with 80 % acetone in water and eluted with the same solvent. after concentrating and drying, relatively pure fr - 008 was obtained. hplc assay a comparison between antibiotic fr - 008 and candicidin has been made by studying the hplc separation profiles of antibiotic fr - 008, candicidin and a mixture of both
對鏈黴菌fr - 008和灰色鏈黴菌imru3570的rflp分析發現，它們的染色體具有很高同源性； pfge的比較研究發現，這兩個菌株的明顯區別是鏈黴菌fr - 008攜帶有兩個線性質粒，而在灰色鏈黴菌imru3570中則沒有這兩個線性質粒。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Dnd phenotype could be detected when the entire dnd cluster was integrated in the chromosome or carried by the low copy - number plasmid in zx1. however, when dnd cluster was carried by a plasmid with a copy - number around or higher than c. 10 copies in zx1, dnd phenotype could not be observed
當完整的dnd基因簇整合在zx1染色體上或由1 2個拷貝的自主復制的質粒攜帶進入zx1 ，都能在zx1中檢測到dnd表型，但是由10個或超過10個拷貝的質粒攜帶進入zx1 ，就檢測不到dnd表型。The results showed that pcr applify a 485 specific molecular band in 672 individuals, the rate of positive reaction is 60 %, southern blot result shows a strong signal in transgenic fish. it is concluded that hu - - ifn gene has been integrated and expression. it is also that foreign gene integrating position and copy number is different individuals, by elis a detecting, 23. 5 % transgenic fish " were detected hu - - ifn gene expression in all 672 transgenic fish, but expression level of hu - - dfn is significant difference in different individuals and growth period
2000年共顯微注射32197粒草魚受精卵，出苗12945尾草魚魚苗，經孵化培育最後獲得1120尾五寸左右草魚，對1120尾草魚提取血液總dna進行pcr和southern雜交檢測， 2000年轉導的草魚的pcr檢測結果，有672尾草魚即600k轉化個體的總dna能擴增出一條485kb的特異分子帶，說明轉化個體整合有huj ifn基因，經southern雜交進一步證實，轉入的抗病相關基因己在轉基因草魚體內染色體上得以整合，但整合位點沒有固定區域，整合的拷貝數存在較大差異。The chief aim of the present study was to primarily explore the early symbiotic interaction between m. huakuii and a. sinicus, using gfp and its mutants as reporter genes. the feasibility of broad - host - range plasmid ptr102 as a stable transfer vector in molecular biology of m huakuii was analyzed
採用發光酶發光活性和-葡萄糖苷酶組織染色法比較研究了攜帶報告基因luxab和gusa表達單元的ptr102標記質粒phn106和phn109在華癸中生根瘤菌7653r中的穩定性。分享友人