染色體篩選 的英文怎麼說

中文拼音 [rǎnshǎitǐshāixuǎn]
染色體篩選 英文
chromosome screening
  • 染 : Ⅰ動詞1 （用染料著色）dye 2 （感染） catch [contract] （a disease） 3 （沾染） acquire （a bad hab...
  • 色 : 色名詞[口語] （顏色） colour
  • 體 : 體構詞成分。
  • 篩 : 名詞[書面語] （植物名） sedge
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 染色體 : [生物學] chromosome染色體疾病 chromosomal disorders; 染色體異常 chromosome abnormality
  • 染色 : dye; dyeing; colouration; tintage; tinging; dyschroia; colouring; colour; [半] decoration染色不足...
  • 篩選 : dressing by screening; screen; preparation by screening; preparation; choose by means of a sift; ...
  1. In this study a microbial system for biphenyl biodegradation is set up in order to investigate the effects of surfactants on biodegradation of hydrophobic organic compounds. four strains which can grow on biphenyl as the sole carbon and energy sources are selected out, in which alcaligenes eutrophus dj1 and pseudomonas sms02 are chosen as degradation strains. after studing the bioavailability of three nonionic surfactants ( op - 10, tween - 80, and triton x - 100 ), they are added into the biodegradation system of biphenyl

    為了考察表面活性劑對疏水性有機污染物生物降解的影響，本論文選擇聯苯作為模擬研究體系，篩選、馴化得到四株可利用聯苯作為碳源生長的菌株；考察了聯苯降解菌株對三種非離子表面活性劑op - 10 、 tween - 80 、 tritonx - 100的生物可利用性；用高效液相色譜法測定了非離子表面活性劑對聯苯的增溶曲線；較系統地研究了加入非離子表面活性劑對聯苯生物降解速率的影響。
  2. The topics include : structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease

    主題包括：基因、染色體與基因組的結構和功能；來自於基因重組、突變和篩選的生物變異；族群遺傳學；運用遺傳學的方法分析蛋白質的功能，基因的調控和遺傳性疾病。
  3. Studies of genes related to heart development in drosophila contribute to reveal the mechanisms of human heart development and the congenital heart diseases. to clone and identify new genes that control the heart development, by a way of chemical mutagen, ems, we have established 1, 200 balanced - lethal lines on chromosome 2 and 3. with the screening the 330 stocks with immunochemical method using heart - specific antibody, mab. no. 3, we detected 60 lethal lines showing heart mutant phynotype

    為了克隆和鑒定控制心臟發育的新基因，本研究利用化學誘變劑甲磺酸乙酯大規模地誘變果蠅，並且建立了1200個第二和第三染色體的平衡致死系，利用心臟組織特異抗體mab . no . 3對其中330個品系進行免疫化學方法篩選，觀察到有60個致死系表現出心臟突變表型， 20個品系的心臟突變表型有待進一步證實。
  4. After chromosome doubling and electric conductivity measuring, 9 variants with higher stability of cell membrane to heat stress than the original varieties were selected

    經過染色體加倍后，通過熱脅迫條件下電導率的測定，篩選出9份細胞膜熱穩定性比原始品種明顯提高的變異體材料。
  5. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  6. Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque

    利用脂質體介導的方法，將pltk - ha與prvbartha - k61基因組共轉染于亞單層vero細胞，依據報告基因lacz在細胞中的表達，篩選藍色蝕斑的重組病毒，經數次蝕斑純化后， pcr鑒定、 western - blot分析。
  7. All these results show that tyrp1 expression is consistent with the melanin accumulation time. ( 4 ) started with aco1 ( aconitase i ), we got positive clone of aco1 then obtained 16 clones by chromosome walking

    （ 4 ）以離id最近的aco1 （ aconitase1 ，順烏頭酸酶）基因為起點，設計引物篩選含aco1的bac克隆，用染色體步行的方法得到16個bac克隆。
  8. The work on physical mapping of the chromosome of s. nanchangensis ns3226 was initiated. nearly a full set of chromosomal asei - bamhi fragments of s. nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library. thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion, suggesting distinct identifications

    此外，還開展了南昌鏈黴菌ns3226染色體物理圖譜構建的前期研究工作：基本克隆到了南昌鏈黴菌ns3226染色體上全套的ase - bamh片段，以它們為探針從南昌鏈黴菌ns3226的基因文庫中釣到164個陽性克隆，並從中篩選到34個ase linkingcosmids ，用bamh進行初步的酶譜分析，結果表明其中有20個cosmids的bamh酶譜相互間沒有明顯的重疊性。
  9. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
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