核異構物 的英文怎麼說
中文拼音 [héyìgòuwù]
核異構物
英文
isomer， nuclear-
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。In this thesis, the development of emulsion polymerization theory and related new technologies are comprehensively reviewed, and the synthetic technologies of acrylate latex modified by organosilicon both at home and abroad are also reviewed. based on the current development and research in this field, attentions of this study are focused on three ways for preparing novel acrylate latexes modified by organosilicons, that is, 1 ) the acrylate monomers are copolymerized with unsaturated silicons by emulsion copolymerization ; 2 ) a new latex with microphase separation morphology is synthesized, which shows a core - shell structure character ; 3 ) soap - free polymerization is explored for preparing a higher - performance latex using an unsaturated silicon monomer, different acrylates and a reactive emulsifier. by using techniques of delaying addition of organosilicon monomer and a hydrolysis inhibitor, the hydrolysis and condensation of 3 - ( trimethoxysilyl ) propyl methacrylate during polymerization can be effectively prevented, as a result, organosilicon content in the macromolecular chain is increased
同時採用有機硅單體延遲滴加及添加水解抑制劑等技術，有效防止了-甲基丙烯酰氧基丙基三甲氧基硅烷在乳液聚合過程中的過渡水解及縮合反應，提高了聚合物大分子鏈中有機硅鏈節的含量；利用紅外光譜與差示掃描量熱儀對產物分子結構進行了表徵，並通過對共聚產物力學和吸水率的測試，證實了本研究所制備的有機硅改性丙烯酸酯共聚物具有比純丙烯酸酯聚合物更優良的力學及耐水性能；從分子設計角度出發，利用醇解反應合成出水解、縮合反應速率較慢的兩種新型不飽和有機硅單體： ?甲基丙烯酰氧基丙基三乙氧基硅烷和?甲基丙烯酰氧基丙基三異丙氧基硅烷；利用核磁共振對新型硅烷單體結構進行了證實；研究表明利用新型硅烷單體可以制備出高硅烷含量的有機硅改性丙烯酸酯乳液，且其聚合產物具有較高的力學及耐水性能。With the rapid development of molecular biology, the researchers of different scientific background are provided with a good opportunity to enter the field. people can resolve some important difficult problems with all kinds of research methods and knowledge in their fields. it is chemists " tribute that they design and synthesize effective nucleic acid cleavage reagents and clarify the reaction mechanism of complexes and dna, which makes it possible to search effective remedial reagents and structural probes by molecular design
分子生物學的迅猛發展為不同科學背景的研究者涉足該領域提供了良好的機遇，人們可以利用各自領域的研究方法和知識來攻克生物學中的一些重要難題，化學家所能做的貢獻就是設計和合成一些特異識別和高效切割的核酸斷裂試劑，並闡明其作用機理，從而使通過分子設計尋找有效的治療試劑和結構探針成為可能。Cadmium zinc telluride ( cd1 - xznxte or czt ) single crystal is one of the three element compound semiconductor materials with great performances used for the detection of x - ray and gamma - ray at room temperature
碲鋅鎘（ cd _ （ 1 - x ） zn _ xte ，簡寫czt ）單晶體是一種性能優異的三元化合物半導體室溫核輻射探測器材料，具有閃鋅礦型的面心立方結構。The percentage of polymorphic sites, degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method. the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified. these sequences, together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics
汝us價ay1830 ）各12個個體進行rapd分析，對比多態位點比例、遺傳多態度以及遺傳距離，並構建neighbor - join噸系統樹;通過pcr擴增出線粒體165rrna基因，純化后直接測序，利用生物信息學方法進行序列分析和核昔酸變異比較，結合ge紅bar止中大西洋叉尾帶魚（ lepid （護腳caud玫tuseuphrasen1788 ）同源序列構建u甲cm叭系統樹。Crystal structure of trinuclear cobalt carbonyl sulfur clusters with a bridging thiocarboxamido group
含橋基異丙基硫代甲酰胺基團配位的三核鈷羰基硫簇合物的晶體結構This course includes : the understanding of biomolecules ; the difference between prokaryotic and eukaryotic cell ; the structure of amino acids and their characteristics ; the stereo - structure of protein ; cell membrane and lipids ; the activity of enzyme ; the activity of nucleic acid ; the application of biotechnology
本課程主要包括有生物分子之了解，真核與原核生物之異同;胺基酸之結構及性質;蛋白質與其空間構型;脂質及細胞膜;酵素之作用;核酸之作用;生物技術之應用。The result indicated that the nadph - dstained neurons and fibers were found in discrete regions throughout the snake brain, such as cerebral hemisphere, supraoptic nucleus, paraventricular nucleus, subcommissural organ, medial longitudinal fasciculus, periventricular grey, locus coeruleus and mesencephalic reticular formation
結果表明：陽性反應主要位於腦的大腦半球、視上核、室旁核、聯合下器官、內側縱束、室旁灰質、藍斑以及網狀結構等區域，比較討論了與其他脊椎動物的異同。Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1
本研究根據genbank發表的gpvb株全基因序列，藉助oligo4 . 1軟體設計一對引物，採用pcr技術擴增gpvh1株非結構蛋白ns2基因，並與pmd18 - t載體連接后測序，結果表明：鵝細小病毒h1株ns2基因核苷酸全長1356bp ，編碼451個氨基酸殘基，與gpvb株的ns2基因相比，核苷酸數目相同，有17個堿基、 6個氨基酸的差異；同源性分析表明：二者核苷酸序列同源性為98 . 75 ，推導氨基酸序列同源性為98 . 67 。According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat, we designed primers and got the structure gene successfully. by 3 ' - race method combined with nested pcr, the 3 ' - end nuclear acid sequence was also obtained ; in additon, for the 5 ' - end sequence, we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer, and till now, partial 5 ' - end sequence has been amplified as well
本研究根據先前分離純化所得天然tb22kda蛋白經maldi - tof - ms （質譜法）測得的氨基酸序列和文獻報道的過敏蛋白核苷酸序列設計引物，擴增克隆了該過敏蛋白結構基因的編碼序列；根據測得的序列設計特異性引物，並利用3 』 - race方法結合巢式pcr擴增得到基因的3 』末端；依據同源性比較的結果選用一段保守序列為5 』引物，並根據結構基因內部序列設計3 』特異性引物，進一步獲得了該基因5 』端的部分序列。分享友人