核移植 的英文怎麼說
中文拼音 [héyízhí]
核移植
英文
nuclear transfer-
The process that led to dolly began with the transfer of the nucleus of an adult cell to an unfertilized egg taken from a donor animal by a process known as cell fusion
多利誕生的過程是這樣開始的：通過一個叫細胞合成的過程，把一個成體細胞核移植到一個取自供體動物的未受精的卵子上。Traditionally, somatic nuclear transfer in mammal is completed by micromanipulation. but micromanipulation is evidently damaging to cytoplast cytoskelton, causing the lost of some important factors involved in programming of donor karyoplast, reducing the volume of cytoplasm
傳統核移植總效率低，且需要熟練的顯微操作技術，所需設備昂貴，限制了核移植的廣泛應用；因此作為替代，化學去核應運而生。Reconstruction of mouse embryos with chemically enucleated oocytes
化學去核卵母細胞為受體的小鼠體細胞核移植Within 2h isolation from the follicle 100 % of oocytes underwent germinal vesicle breakdown and both the nucleolus and the nuclear envelope disappeared. after culturing for 6 h, prometaphase occurred in 90 % oocytes
本研究旨在探索一種嶄新的、化學試劑誘導去核卵母細胞為核受體的、無透明帶的、手工體細胞核移植方法。Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency
多年來，國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究，目前這項技術已達到實用水平，並日益發揮作用；二是對魚類培養的組織細胞冷凍保存，通過魚類細胞的培養、超低溫凍存、解凍后再培養過程，結合細胞工程技術（如核移植、電融合等）進行體細胞育種；大量的研究結果表明魚類體細胞核具有發育的全能性，隨著細胞培養技術、細胞工程技術日益發展成熟，完全具備實現魚類物種種質長期保存的理論基礎和技術條件。6 - dmap + ccb + ethanol can effectively activate the reconstructed rat - mouse embryos. 5 ) 225 reconstructed embryos from 2 - cell to morula were transferred into 46 recipients
23 l6 dmap乙醇ccb能有效地激活種間核移植重構胚。The results show that the development rates of morula treated with serum starvation for 3d, 4d, 5d are higher apparently than nocodazole group and control group ( p < 0. 05 )
直接注射法構建的核移植重組胚胎的桑堪胚發育率（ 25 95 ）高於電融合法（ 16 07 ） ，統計學分析差異顯著（ p 0This simple two - step chemical enucleation procedure yields fully enucleated mouse oocytes in 85. 6 % of cumulus - oocytes complexes and 56 % of natural oocytes, significantly different ( p < 0. 05 ). since the enucleation rate of cumulus - oodles complexes is equivalent to that by micromanipulation, induced enucleation will greatly improve the whole efficiency in mouse somatic nulear transfer
該方法完全丟棄了傳統核移植的顯微操作及其繁瑣程序，是純粹的手工克隆（ hmc ） ，它的成功將會大大簡化核移植程序，提高核移植效率，加快其產業化的步伐。These observations show that reprogramming is easier in interspecific embryos reconstructed with es cells than that reconstructed with somatic cells, and that es cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. oocytes were reconstructed with outbreeding kunming albino mouse es cells and enucleated rabbit oocytes, and the effects of the passages of es cells and 6 - dmap on the development of interspecific reconstructed oocytes were analyzed. the interspecific reconstructed es - rabbit oocytes were activated either by combined two set electric pulses and 6 - dmap or by two set electric pulses
以上結果顯示， 6 dmap能增加胚胎幹細胞異種重構卵的卵裂率，對重構卵囊胚的發育率影響不大；高培養代數和低培養代數的胚胎幹細胞用於異種核移植不影響異種重構卵的卵裂率和囊胚發育率；用胚胎幹細胞作為供核細胞；比體細胞作為供核細胞所構建的異種重構胚更容易進行重編程，並且胚胎于細胞指導異種克隆胚胎正常發育的能力強于體細胞。There were no significant difference in fusion rate, cleavage rate and blastocyte development rate with different enucleation time of oocytes during ivm16 - 24h ( p > 0. 05 )
在體外成熟16 24h期間，水牛卵母細胞去核時間的不同對核移植后的融合率，分裂率和囊胚發育率沒有顯著影響。A preliminary study on porcine cloning via cumulus cell nuclear transplantation
卵丘顆粒細胞核移植克隆豬的初步研究Effects of different electrofusion parameters on activation and early development of mouse embryos reconstructed by cumulus cell nuclear transfer
不同電融合條件對小鼠卵丘細胞核移植重組胚融合和早期發育的影響The purposes of this experiment were to establish cuture systems of fibroblasts and methods of cell cycle synchronization so as to provide better donor cells for somatic nuclear transfer
本實驗旨在建立成纖維細胞的培養系統和細胞周期同步化方法從而為體細胞核移植提供良好的供體細胞。But they used the so - called nuclear transfer technique applied in 1997 by the scottish scientists when their made dolly the sheep
而他們使用的這種所謂的核移植技術正是由蘇格蘭科學家於1997年克隆多利羊時建立和應用的技術。This was the first successful nuclear transplant involving cells of a warm-blooded animal.
這是溫血動物細胞核移植試驗的首次成功。Clones are lost from the earliest developmental stages and throughout pregnancy. now more and more studies support the evidence on the failure of cloning : chromatin inappropriate epigenetic reprogramming, and then result in abnormal genes expression
大量的研究表明，克隆動物在核移植后其染色質的再程序化表現異常，使細胞不能成功地通過調節轉錄活性而有效地控制其發育分化。Studies on superovulation in cats fifty nine cats ( 45 females, 5 males ) were used in this study
5522石5枚。 （四）黑熊家貓種間體細胞核移植研究主要結果14. rat - bovine interspecies somatic nuclear transfer 1 ) bovine cocs had been cultured in the maturation medium containing 8 - 10 % ocs in vitro for 22 - 24h, 60. 86 % - 61. 41 % of maturation oocytes can be obtained
（四）大鼠牛種間體細胞核移植研究的主要結果牛卵母細胞在含8 10發情牛血清（ ocs ）的成熟培養基培養22 24h ，能使60The main contents include : 1 ) the transplant of bsp ( board support program ) armboot. 2 ) the transplant of an embedded - linux operating system. 3 ) the driver development of ralink wireless lan card ( support 802. 11g ) use to wireless data transmission on the actual process
本文則主要闡述作者完成的操作系統移植及無線網卡驅動程序開發部分，主要內容包括: 1 ）基於這一系統的板載支持程序（ bsp ） armboot的移植。 2 ）嵌入式linux操作系統內核移植到系統上的方法。2 ) we got 55. 08 % of fusion rate when reconstructed embryos were fused with 1500 - 1800v / cm voltage and 50ms impulse. in addition, a same result can be obtained with low voltage + length impulse or strong voltage + short impulse
41的卵母細胞達到成熟：在1500 1800v cm ， 30 40sin條件下，大鼠牛核移植重構胚能達到50廠0 55分享友人