核蛋白體亞基 的英文怎麼說

中文拼音 [hédànbáitǐyǎjī]
核蛋白體亞基 英文
ribosomal particle
  • 核 : 核構詞成分。
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 體 : 體構詞成分。
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. In addition, it was also found that this protein shares limited but significant homology with the sam - dependent methyltransferase of mesorhizobium sp. bnc1 ( 32 % similarity ), and the similarity of its 303 - 362 region to the 160 - 220 domains of l11 methyltransferases of e. coli ( prma ) is 41 %. it is suggested that methylation of l11 resulted in effects of noea on nodulation of 042bm

    發現noea與中慢生根瘤菌（ mesorhizobiumsp . ） bnc1可能的sam -依賴性的甲基轉移酶相似性為32 ，而其303 - 362區域與大腸桿菌（ escherichiacoli ）的核糖體50s亞基的l11蛋白甲基轉移酶（ prma ）的160 - 220結構域的相似性達到41 。
  3. 1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out

    具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。
  4. Chloroplast ribosomes contain about 50 distinct ribosomal proteins, distributed between the two subunits.

    葉綠體核糖體還含有約50種不同的核糖體蛋白，這蛋白分佈在兩個亞基之中。
  5. It is toxic for most mammalian cells since ricin a chain ( rta ) is an rna specific n - glycosidase that removes a specific adenine residue in a highly conservative region from among over 4000 nucloside residues present in 28s rrna, and causes protein synthesis inhibition and cell death

    Rta具有n -糖苷酶活性，可催化28srrna在4234位脫去腺嘌呤，使核糖體60s亞基失活，從而抑制蛋白質合成，導致細胞死亡。 b鏈（ rtb ）是結合鏈，能和細胞表面半乳糖受體結合，協助a鏈進入細胞內。
  6. Ricin a chain ( rta ) is an active chain and has specific n - glycosidase activity, which excises a specific adenine residue ( a4324 in rat ) from a highly conserved loop of 26 or 28s rrna in 60s ribosomal subunits. this cleavage of adenine can lead to the disruption of ribosomal function, thereby, inhibits the protein synthesis and then cause the death of cells

    A鏈是活性鏈，具有n -糖苷酶活性，可催化切斷真核生物60s亞基28srrna中第4324位腺嘌呤與核糖分子之間的糖苷鍵，使其脫去一個腺嘌呤，使核糖體60s亞基失活，從而抑制蛋白質的合成。
  7. By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature

    利用dna重組技術，將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb ， iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白，光密度掃描對表達產物進行初步定量，表明表達產物約占菌體總蛋白的14 。
  8. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
  9. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  10. All the isolates in the study of genotype vii could be recoganized by the new cutting site at nt 872 by re hinf i which is absent in the genetic supgroups viia, viib, and group vi. the amino acid sequences of the coding region of the f gene were deduced

    由核昔酸序列推導了f蛋白高變區1一125位氨基酸（ aa ） ，通過分析比較發現了新的基因型和基因亞型與其他基因型毒株的差異還體現在aa位點的變化上。
  11. Pcr products were inserted into pbv - 220 with double digestion of restriction enduonuclease. the expression vectors of pbv - a pbv - b and pbv - c were constructed by orientaional cloning. through sds - page, bioactivity and function analysis of expressed protein, the function of phaa, phab and phac was confirmed

    菌株的亞克隆基因組片段中，分離出phaa 、 phab和phac三個基因片段，定向克隆至原核表達載體pbv220上，構建了三個原核生物表達載體pbv - a 、 pbv - b和pbv - c ，通過對表達載體誘導表達，表達蛋白產物的sds - page分析、生物活性與功能分析，確定了基因phaa 、 phab和phac的生物學功能。
  12. The two genes were subcloned into pet30 vector, and recombinant proteins with predicted molecular weight were achieved. in this paper, we constructed the recombinant e. coli highly expressing pil - 10 in order to study the pleiotropic immunological regulations in various diseases

    然後將p35基因和p40基因分別亞克隆到原核表達載體pet30c 、 pet30b中，用iptg誘導培養，分別表達出了預期分子量大小的重組蛋白。
  13. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  14. Subcloning of 32kda proteins gene of schistosoma japonicum in the eukaryocyte expression vector

    蛋白質基因在真核表達載體中的亞克隆
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