核酸內切酶 的英文怎麼說

中文拼音 [hésuānnèiqiē]
核酸內切酶 英文
nickase
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 內 : 名詞1. （內部; 里頭; 里邊） inner; inside; within 2. （妻子或妻子的親屬） one's wife or her relatives 3. （姓氏） a surname
  • 切 : 切Ⅰ動詞1 （合; 符合） correspond to; be close to 2 （用在反切后頭 表示前兩個字是注音用的反切）見 ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  1. Found the phenomenon observed in the nematode caenorhabditis elegans for the first time in 1998, consequently similar processes have been described for drosophila melanogaster, trypanosome, mammals including humans. the mechanism is that sirnas is the mediator, which can induce the risc to the target mrna and degrade it. recently there was great progress in the specific gene therapy and anti - virus, and rnai has been a focus of rna molecular therapy

    自1998年fire等在研究線蟲時首次發現以來，相繼在果蠅、渦蟲、錐蟲、小鼠及哺乳動物細胞中發現rnai現象。一般認為： rnai第四軍醫大學碩士學位論文效應作用機制是sirnaskmallinterferinguaduplex ）作為中介分于，引導risc （ rnaiinducingsuppresscomplex ）至靶基因m洲a處，隨后核酸內切酶將之降解。
  2. The deduced amino acid sequence of hau3r protein suggested that propablely it is a kind of endonuclease similar to ea59

    根據其核苷酸序列推譯的氨基酸序列暗示hau3 ~ r蛋白可能是一種類似於ea59的核酸內切酶。
  3. Methods isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test, plasmid profiles, restriction enzyme fingerprinting assay and plasmid elimination method

    方法對我院不動桿菌的感染進行調查，採用藥敏試驗、質粒抽提和限制性核酸內切酶分析法、質粒消除試驗。
  4. Using the rt - pcr technique and treatment with endonuclease, the transport of irxi mrna from scion into stock were detected. in the hetrograft combinations, c24 on lew2 - 1 and lew2 - 1 on at9, no evidence was found that irx1 mrna could be transported between the graft partners

    結合rt - pcr技術和核酸內切酶酶切檢測，在上述嫁接組合的砧木lew2 - 1突變體中檢測到了突變基因irx1的mrna ，在lew2 - 1 at9嫁接組合的接穗中和c24 lew2 - 1嫁接組合的砧木中檢測不到irx1的mrna 。
  5. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  6. The insert dna fragments are 7kb and llkb, respectively. two subclones that were designated pgr3h1 and pgr7h1 and can increase glyphosate resistance of e. coli jm109 up to 150mm glyphosate were constructed by subcloning the 2. 4kb and 3. 2 kb hind ? / psti fragments of pgr3 and pgr7 into the corresponding sites of pgem - 3zf and pbluescript. sequence analysis of these two subclones revealed a completely identical 1323bp open reading frame that encodes an epsp synthase

    以pgem - 3zf和pbluescript為載體，利用限制性內切酶hind和pst構建這兩個克隆的亞克隆，從中分別得到兩個草甘膦耐受亞克隆pgr3h1和pgr7h1 ，插入片段各為2 . 4kb和3 . 2kb ，對這兩個亞克隆進行序列分析，發現二者均含有一個核苷酸序列完全相同的完整的epsp合成酶基因? ? aroa ，其核苷酸序列長為1323bp ，推導的epsp合成酶由441個氨基酸組成，兩個亞克隆的草甘膦耐受濃度最大可達150mm 。
分享友人
分享到 Line

分享到臉書