核酸復性 的英文怎麼說
中文拼音 [hésuānfùxìng]
核酸復性
英文
nucleic acid renaturation-
Through the analyzing the macro - structure to micro - structure, the author considers that retarding mechanism of citric acid is that citric acid and ca in the gypsum form the complexant ion, and hinder the crystallization center of dihydrate crystal bigger ; that of sodium tripolyphosphate is that it combined with ca, hinders the dissolve of hemihydrate. formation and growth of crystallization center ; that of bone glue is the glue - protection and chemical absorption action of active group, which also retards the formation and growth of crystallization center
通過宏觀到微觀的分析，筆者認為，檸檬酸的緩凝作用的原因主要在於檸檬酸與鈣形成絡合離子，影響了二水石膏晶體的晶核長大過程；多聚磷酸鈉與鈣形成某種復鹽，對于石膏晶體的溶解、成核和長大過程均有強烈的阻礙影響；骨膠則在於膠體對半水石膏的包裹和活性基團的化學吸附，使二水石膏晶體的成核和長大困難。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。Study on in viro drug delivery and repairing large segmental infected bony defect with massive reconstituted bovine xenograft aided by calcium phosphate cement drug core
磷酸鈣骨水泥載藥核心的塊型重組合異種骨體內緩釋及修復兔長段感染性骨缺損的研究Histidine a basic amino acid formed only rarely in proteins. it is formed from atp and 5 - phosphoribosyl pyrophosphate in a complex biosynthetic pathway. glutamate is an intermediate in its breakdown
組氨酸：是蛋白質中少有的一種堿性氨基酸。由atp和5 -磷酸核糖基焦磷酸鹽通過復雜的生物合成途徑合成。在其降解過程中可形成谷氨酸。Serial studies on relationship between nucleotide excision repair system gene expression and chemotherapeutic
核苷酸切除修復系統基因表達水平與化療藥物耐藥相關性的系列研究The reproductive organ blister measles therefore recur, is because blister measles virus deep hiding in ganglion " the establishment gram kj medicinal preparation " series medicineis one kind of structure medicine, it ordinary disease - resistant poisonous medicine composition member is younger than several hundred times, can seep the nerve and the ganglion from the extroversion which suffers injury, is same along with it to sponge absoring water, layer upon layer strips the adsorption in the ganglion the crazy duplication viral body, the destruction virus s nucleotide duplication enzyme, causes it to be separated from the nerve is separated from the virus can massive gathering in the reproductive organ hypodermic, by now again coordinated the establishment gram venereal diseases kj medicinal preparation formidable anti - virus function, comprehensively struck kills the virus, caused the virus not to hide the place, thus achieved thoroughly permanently cured goal
安立克kj劑"系列藥物是一種微分子結構的藥物，它比普通抗病毒藥物的組成分子小幾百倍，能夠從外向內滲透進受損的神經和神經節，隨之就向海綿吸水一樣，層層剝離吸附在神經節里瘋狂復制的病毒體，破壞病毒的核苷酸復制酶，使其脫離神經.脫離出來的病毒會大量的聚集在生殖器皮下，這時再配合安立克性病kj劑強大的抗病毒作用，全面擊殺病毒，使病毒無藏身之地，從而達到徹底根治的目的A survey of literature shows that little study has been done on uptake of pollutants by plants from the polygonaceae, and that the reports about their physiological and biochemical characteristics under heavy metal stress were sporadically seen. a batch of sand culture and soil culture experiments were carried out to investigate heavy metal and radiocesium uptak e and accumulation by rumex acetosa linn, polygonum microcephalum d. don, and rumex hastatus d. don widely distributed on copper mining areas, and their physiological and biochemical characteristics under cu zn stress, as well their potentials in application to phytoremediation
本論文以廣泛生長于銅礦區的蓼科植物酸模、小頭蓼和戟葉酸模為試驗材料，運用砂培、土培等方法，研究它們對重金屬（ cu 、 zn ）以及放射性核素（ ~ （ 134 ） cs ）的吸收和積累特性以及cu 、 zn脅迫條件下它們的生理生化特徵，初步探討它們在無機污染物污染土壤植物修復中的應用潛力。In this study, it has been put forward that taking reactive nanometer magnetic fe304 particles as magnetic nucleus, and the copolymer of styrene ( st ) ? crylic acid ( aa ) as macromolecular shell, we could synthesize, magnetic polymer composite microspheres containing carboxyl groups on their surface, then microspheres are activated by thionylchloride, the surface of such magnetic composite microspheres thus produced had reactive acid chloride groups which then react with the free amino groups of the free soluble enzymes to give peptide bonds ( ? o ? h ?,
本研究首次提出了以納米級磁性fe _ 3o _ 4粒子為核心，苯乙烯（ st ） ?丙烯酸（ aa ）共聚物為高分子殼層，合成了表面帶羧基的磁性高分子復合微球，然後將這種微球用二氯亞碸進行活化處理，在其表面形成了反應性酰氯基團，該基團可以與游離酶的氨基形成肽鍵，從而將游離酶固定化。Fxs is mainly caused by a kind of dynamic mutation, the expansion of a cgg repeat located in the 5 ' - untranslated region ( 5 ' - utr ) of the fmr1 ( fragile x mental retardation ) gene
該綜合癥由患者細胞中可見xq27 . 3的脆性位點fraxa而得名。絕大多數脆性x綜合征患者的發病是由於fmr1基因5端非翻譯區的三核苷酸cgg重復的不穩定擴增所致。2 with dispersion - polymerization, taking above reactive nanometer magnetic fe304particles as nucleus, and the copolymer of styrene ( st ) ? crylic acid ( aa ) as macromolecular shell, we could synthesize, magnetic polymer composite microspheres containing carboxyl groups on their surface
2採用分散聚合法，以上述反應型納米磁性fe _ 3o _ 4粒子為核心，苯乙烯（ st ） ?丙烯酸（ aa ）共聚物為高分子殼層，合成了表面帶羧基的磁性高分子復合微球。The comparative analysis of sequences indicated that the sequences of meq in different pathotypes are relatively conserved and the homology of the amino acid sequences is very high. the significant differences include two mutations in both mdv - 1 vaccine strains cvi988 / rispens and 814 strain : the deletion of a proline ( no. l93aa ), and this mutation is just exactly located in a 15 - amino - acid ( eelcaqlcstppppi ) repeat sequence within the c - terminal transactivation domain of meq protein ; and a point mutation with a shift from alanine ( a ) of all virulent strains to serine ( s ) was occurred on the no. 71 aa
結果發現， mdv不同致病型的meq基因序列相對比較保守，它們相互間核苷酸和氨基酸序列的同源性均很高；但是，與所有七個致瘤性的mdv毒株相比，二個型弱毒疫苗cv1988 rispens株和814株均出現了兩個特徵性的突變：即第194位的p缺失性突變和第71位氨基酸由a變成了s的位點突變；缺失性突變恰恰位於meq基因中轉錄激活域內的一個多脯氨酸的重復序列（ pppp ）之中。The accuracy and stability of the typing result typing results match the set results, and in 5 hybridization, the repeated rate is 91. 2 %
3寡核營酸晶元分型結果的準確性與穩定性分型結果與dna測序相符合，重復5次雜交重現率91 . 2 % 。That is to add a special fluorescence - based dna internal standard in the telomerase elongated ts primers, then do pcr amplification after a step of refinement ( hydroxybenzene / chloroform extracting, and deposited by ethanol ). sequencing analysis of pcr product was done on 310 gene scan analysis ?. 1. 2 dna sequencer to determine telomerase activity. notably, this method eliminated the restraining factors of taq dna polymerase, making it possible to erase the sample differences met in pcr and eradicate the annoying phenomena of pseudo negative results
在kim等開發的端粒重復擴增分析法（ trap ）的基礎上進行改進，即通過對端粒酶延伸ts寡核昔酸反應產物的精製，消除了pcr擴增中抑制taq酶活性的因素，從而減少了樣品之間pcr擴增上的差異和假陰性現象的發生，提高了判斷樣品端粒酶陰、陽性的準確率和定量的準確性。The effective method of nucleation and growth of nanoparticles as applied to synthesize w - and mo - containing polyoxometalates nanoparticles in precursor films by making use of their acidity or oxidative property. the controllable synthesis of nanoparticles can be achieved by increasing synthetic cycle of polyoxometalates. the composition, structure, and properties of the as prepared composite films have been characterized in detail by uv - vis, ftir, x - ray photoelectron spectra, scanning electron microscopy, atomic force microscopy, transmission electron microscopy and cycle voltammograms
利用有效的在前體膜中成核、生長納米粒子的方法，使用keggin型鎢系、鉬系多酸，有目的地利用其酸性，強氧化性，在膜中發生反應，從合成出基於多酸的納米粒子，通過增加多酸的反應循環數，我們可以實現了納米粒子的可控合成，採用uv - vis光譜、 ftir光譜、 x -射線光電子能譜、掃描電子顯微鏡、原子力顯微鏡、透射電子顯微鏡、循環伏安對所制備的納米復合膜進行了組成、結構和性質表徵。Consequently, in our study we propose a new idea for locating interactive multiple genes and constructing their interactive network responsible to complex disease utilizing single - nucleotide polymorphisms ( snps ). we demonstrate the properties of this novel approach via an application to the alcoholism data in gaw14
在本文的研究中我們提出了利用單核苷酸多態性（ snps ）標記對復雜疾病基因作圖（定位）及互作網路構建的新方法，並將該方法應用於gaw14發布的酒精中毒數據相關互作多基因定位問題上，取得了良好的效果。分享友人