核酸擴增 的英文怎麼說

中文拼音 [hésuānkuòzēng]
核酸擴增 英文
nucleic acid amplification
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 擴 : 動詞（擴大） expand; enlarge; extend
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  1. More and more fluorescent signal can be collected with the pcr reaction carry on. the method is more automatized and much less time consumption ( only 3 hours from nucleotide hybridization capture to result found )

    這樣利用熒光信號積累可以實時監測整個pcr進程，實現了pcr擴增和核酸雜交以及熒光電信號放大檢測同步進行的自動化檢測技術；實時熒光pcr技術具有更大的優越性： l ）不需要pcr后處理，
  2. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  3. It ' s meaningful to study the function of rab proteins in ciliates and further to explicit the mechanism of vesicular trafficking. the rob gene was amplified from macronuclear dna of euplotes octocarinatus. the size of gene was 783 bp long with an orf of 624 bp encoding eorabl protein and containing three in - frame tga codes

    本研究利用pcr技術從游仆蟲（ euplotesoctocarinatus ）大核dna中擴增出rab基因，並對該基因進行序列分析，該基因全長為783bp ，兩端為端粒序列，編碼框為624bp ，編碼207個氨基酸，開放讀框中有3個tga ，在此編碼半胱氨酸。
  4. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  5. The main equipment here is a thermal cycler system for nucleic acid amplification ( pcr, quantitative fluorescence detection or common )

    主要儀器就是核酸擴增熱循環儀（ pcr儀，實時熒光或普通的） 。
  6. The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide

    核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。
  7. All kind of oligonucleotides ( 16 sense primers, 8 antisenset primers, 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna. no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations

    依據nbs - lrr類r基因特有的兩個保守序列glpl和cfly （兩者相距約70個氨基酸）合成所有可能的簡並寡核苷酸引物（左引物16個，右引物8個，共組成128個引物組合）對玉米基因組dna進行擴增的結果表明：在6個引物組合中得到了擴增產物，產物長度在500 1000bp之間；沒有獲得特異性擴增產物（指僅從含ht基因材料中得到，長度約210bp的dna片斷） 。
  8. G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software

    本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。
  9. The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again

    應用間接免疫熒光試驗進行血清學鑒定，結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序，在genbank上進行同源性比較，發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高，分別為94 、 95 ，從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上，本實驗對兩株病毒進行了核苷酸全序列測定。
  10. The donkey - adapted strain of eiav ( dv116 ), parental strain of eiav - dla, is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s. the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period. in this study, we constructed a full - length provirus dna clone of dv116 by pcr

    本實驗中將eiav驢強毒dv體外感染驢白細胞培養物，一定時間后收獲病毒（本文中簡稱dv116 ） ，提取eiav前病毒dna ，以pcr法分別擴增並克隆了包含全長基因的三段前病毒dna片段，以雙脫氧法測定了dv116病毒全基因序列共8236個核苷酸。
  11. The percentage of polymorphic sites, degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method. the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified. these sequences, together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics

    汝us價ay1830 ）各12個個體進行rapd分析，對比多態位點比例、遺傳多態度以及遺傳距離，並構建neighbor - join噸系統樹;通過pcr擴增出線粒體165rrna基因，純化后直接測序，利用生物信息學方法進行序列分析和核昔酸變異比較，結合ge紅bar止中大西洋叉尾帶魚（ lepid （護腳caud玫tuseuphrasen1788 ）同源序列構建u甲cm叭系統樹。
  12. In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level

    本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆，通過核酸序列的差異比較分析，研究了該基因在不同物種中的進化關系；同時，通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究，獲得如下研究結果： （ 1 ）通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。
  13. The full length cdnas of 17 - hsd1 and 17 - hsd3, 17 - hsd8 were obtained by library screening and race, respectively. expression patterns ( tissue distribution ) of three types 17 - hsds were checked by rt - pcr and northern blot. the recombinant constructs of 17 - hsdl / pet blue2 and 17 - hsd8 / pcdna 3. 1 were made and subsequently transformed into the corresponding host expression cell of ( de3 ) placi and mammalian hek 293 cell

    首先從genebank下載在其他脊椎動物中已被克隆17 - hsd1 ， 17 - hsd3的氨基酸和核甘酸序列，並在序列保守區域設計簡並引物，然後分別以羅非魚卵巢和精巢cdna為模板進行rt - pcr擴增得到17 - hsd1 ， 17 - hsd3的中間片段。
  14. To organise a memorial event for the public and the carers of pwas

    評估常規血清測定中核酸擴增測試的診斷敏感性和特異性；以及
  15. To evaluate the diagnostic sensitivity and specificity of nat in routine serological assays ; and

    評估常規血清測定中核酸擴增測試的診斷敏感性和特異性以及
  16. To optimize a cost - effective nat protocol suitable for high throughput blood testing centres

    為需要處理大量血液樣本的血液測試中心制定適用的核酸擴增測試規程，以期達到最佳的成本效益。
  17. To optimise a cost - effective nat protocol suitable for high throughput blood testing centres

    為需要處理大量血液樣本的血液測試中心制定適用的核酸擴增測試規程，以期達到最佳的成本效益。
  18. To develop laboratory expertise in nucleic acid amplification test for simultaneous detection of hiv - 1, hbv and hcv in blood donations

    發展核酸擴增測試的化驗技術，以便同時測試捐血者的血液中是否含有1型愛滋病病毒乙型肝炎病毒及丙型肝炎病毒。
  19. The room for the following equipments need the air - conditioning : uv - spectrophotometer, fluorescence spectrometer, gc, gc - ms, aas, afs, icp, icp - ms, hplc, hplc - ms, ion chromatography, atomic fluores - cence spectrometer, pcr amplification and balance

    12紫外/可見分光光度儀、熒光分光光度，氣相色譜，氣相色譜質譜、原子吸收，原子熒光儀、等離子發射光譜、等離子質譜、高效液相、液相色譜質譜、離子色譜、熒光光度、 pcr核酸擴增儀以及天平室安裝空調。
  20. The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification

    本文回顧了李壞死環斑病毒的檢測方法，較全面地評述了國內外病原物檢測技術研究進展：在模板的擴增有各種pcr技術、 nasba技術；在信號的放大有同位素、生物素或dig標記的cdna和crna探針，分支探針和肽核酸探針；模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術；模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。
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