核酸的進化 的英文怎麼說
中文拼音 [hésuāndejìnhuà]
核酸的進化
英文
evolution of nucleic acids-
More and more fluorescent signal can be collected with the pcr reaction carry on. the method is more automatized and much less time consumption ( only 3 hours from nucleotide hybridization capture to result found )
這樣利用熒光信號積累可以實時監測整個pcr進程,實現了pcr擴增和核酸雜交以及熒光電信號放大檢測同步進行的自動化檢測技術;實時熒光pcr技術具有更大的優越性: l )不需要pcr后處理,The changing tendencies of the relative contents of phosphorous contained substances have been detected by in - vivo " p magnetic resonance spectroscopy ( in - vivo " p mrs ) during the whole hatching process. in - vivo ] p mrs proved the catabolism of adenosine 5 ' - triphosphate ( atp ), phosphorous ester and phosphocreatine ( pcr ) when the embryo dead. the results could be used to deduce the conversion of phosphorous contained metabolites during the chicken embryo developed
用活體核磁共振定域氫譜( in - vivohmagneticresonancespectroscopy , in - vivohmrs )對胚胎發育過程中羊水和蛋白、蛋黃的成分進行了分析;用活體磷譜( in - vivo 』 』 pmrs )的方法分析了在整個胚胎發育過程中含磷代謝物的相對含量隨時間的變化,表明了磷脂類物質及三磷酸腺苷( atp ) 、磷酸肌酸( pcr )在此過程中的變化及可能的相互轉化的趨勢,胚胎死亡后的磷譜也證明了磷脂類物質及三磷酸腺苷( atp ) 、磷酸肌酸( pcr )在死亡過程中降解為無機磷的現象。Results show that vp37 protein can bind single strand nucleic acid cooperatively and nonspecificallty, and the vp37 - ssrna complex was stable at high salt concentrations, suggesting vp37 is a possible mp. vp37 is the only protein characterized so far showing rna - binding ability in genus fabavirus
為了驗證vp37是否具有核酸結合能力,我們利用6his - vp37蛋白進行了核酸結合實驗,結果表明vp37是一種能非特異性結合單鏈核酸的蛋白;其在結合核酸時具有協同性; na ~ +的變化對其核酸結合能力影響較小。A chip based on electroelution principle was presented for the recovery of dna fragments, which can make the isolation and collection of target dna fragments possible in a single process by switching electrodes
提出了一種基於電洗脫原理的核酸純化回收晶元,通過對晶元上電極進行適當的切換操作,可一次完成核酸樣品分離和純化回收。From dead chicken which infected infectious stunting syndrom of our province, one virue was isolated using spfeggs, chicken embryo fibroblast, mdck18, and vero cell. this virus was unable to agglutinate chicken erythrocytes. in order to definite the pathogeny of infectious stunting syndrom. physical and chemical specific property, types of the nucleic acid of the isolated virus, recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken
為確定該病的病原,對所分離病毒進行了理化特性測定、病毒核酸型別測定、動物回歸試驗等生物學特性測定,證明該分離病毒與細小病毒科( parvovirdae )細小病毒屬( parvovirus )的雞細小病毒( chickenparvovirus )特性基本相符,核酸型為dna型。So must use information theory method depict and abundant the genetic diversity index system. in addition to, the introduce of molecule biology technology and the research of nucleotide sequence evolutive give a new method for population genetic, so must do deeply research about the analysis method of dna sequence data = the research main about the follows : there are three parts about the information model of population genetic : one about the shannon information entropy property of equilibrium population and the entropy change in the process of establish equilibrium ; another research is about the diversity measure - ment of genetic variation ; lastly, research the shannon information measurement about the disequilibrium gene variation. the result is : 1 to a definite gene distribution, the genotype entropy reach the maximum at the equilibrium population, the process of population from disequilibrium to equilibrium, the entropy get large and large
此外,分子生物技術的介入及核苷酸序列進化的研究都為群體遺傳學的深入研究提供了新的途徑,但關于dna序列數據的分析方法需要作進一步的研究。本研究主要體現在以下幾個方面: (一)關于群體遺傳學的信息論模型研究,主要分為三部分內容:一是群體平衡的shannon信息熵的性質和群體平衡建立的熵變性質;二是群體遺傳多樣性測度的研究;三是非平衡群體的基因變異測量shannon信息量的方法研究。得到了如下結論: 1 、平衡群體的shannon信息熵最大,群體平衡的過程是熵的增大過程。Reconstruction of evolutionary tree based on information about short - range correlation of nucleotides in gene sequence
以核苷酸短程關聯為基礎的進化樹重建In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level
本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆,通過核酸序列的差異比較分析,研究了該基因在不同物種中的進化關系;同時,通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究,獲得如下研究結果: ( 1 )通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
對phz1754進行外切核酸酶( exonuclease , exo )順序缺失,獲得單向長度漸減重疊的系列突變體,核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps , frameplot程序分析揭示出該區域一個完整的開放閱讀框( orf )的存在,其大小為1656bps , g + c含量為70 . 3 ,編碼552個氨基酸,利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較,發現無論在核苷酸水平還是在蛋白水平上,該orf均與膽固醇氧化酶表現出同源性,而且與鏈黴菌膽固醇氧化酶同源性最高,說明該orf編碼膽固醇氧化酶基因。This review focuses on morphological and physiological reactions of fruit tree to water stress. leaf area, root growth and microstructure of leaves and roots were investigated. some physiological and biochemical index of fruit tree leaves and roots under water stress, such as variations of stomatal aperture, photosynthesis, photoinhibition, metabolism of lipoxygenase, content of proline, nuclear acid and endogenous phytohormones, were summarized
從水分脅迫對果樹葉、根的形態指標及顯微結構,葉片氣孔行為、光合作用、光抑制、活性氧代謝、脂氧合酶代謝、多胺代謝、脯氨酸、核酸代謝、內源激素變化等生理生化方面綜述了近十幾年來的研究成果,為全面研究果樹抗旱機理及進一步制定抗旱措施奠定理論基礎。The nucleic acids of the all influenza viruses conducted in the research were extracted from the viruses propagated in specific - pathogen - free chicken embryos. all of the eight segments were amplified by rt - pcr, and the purified pcr products were done cycle sequencing with specific primers, furthermore, the sequencing products were purified and run gel on abi prism 377 dna sequencing machine. the specific primers of the eight genes for pcr and cycle sequencing were designed using the ohgo ( 4. 0 version ) and genedoc ( 2. 3 version ) software
首先將實驗用毒株在spf雞胚中增殖,提取核酸,然後應用oligo ( 4 . 0版本)和gendoc ( 2 . 3版本)軟體設計h9n2aiv所有8個基因片段特異的pcr引物及序列測定引物,通過rt - pcr方法擴增所有毒株的各個基因片段,純化後用特異引物進行測序反應,反應產物純化后在abiprism377dna序列分析儀上進行序列測定,然後應用wisconsinsequenceanalysispackage ( gcg , 10 . 2版本) 、 phylogeneticanalysisusingparsimony ( paup , 4 . 0版本)和treeview ( 1 . 5版本)軟體進行序列的數據編輯、序列翻譯、進化樹繪制和遺傳演化分析。This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv
為了進一步探尋分離株與標準株的異同,又採用rt - pcr方法,擴增獲得分離株f _ o裂解位點附近的基因片段,經測序后與國際上已發表的新城疫病毒的核酸序列進行比較,結果表明其與標準株和疫苗株之間的同源性較低,僅為82 86之間。經系統發育進化樹分析后,判定該分離株為新城疫病毒( ndv )基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析,結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。Several kinds of preparation methods and the applied developments of magnetic polymer microsphere in cell separation, immobilized enzyme, dna separation and medicine carrier are discussed in detail, and the prospects of magnetic polymer microsphere are also proposed
在此基礎上,對磁性高分子微球在細胞分離、固定化酶、靶向藥物、核酸分離等領域的最新應用及存在的問題進行了分析,並指出了該領域今後的研究方向。The rest mutations in pp38 and pp24 are at random. sequence analysis also shows the first 195 nuclear acids of pp38 and pp24 are the same except for the 81 site ( g / c ), but this mutation does not cause the change of amino acid. we regard this as a genetic marker connecting with geography in the evolution of mdv but not related to isolated time and pathotype of different strain of mdv i
對pp24基因和pp38基因進行同源性比較分析的結果表明,絕大多數毒株二者的前195個核苷酸完全一致,不同毒株間的第81位核苷酸的差異( g / c )並不引起編碼的氨基酸變化,僅僅與地域分佈有關,這很可能是mdv在長期病毒衍化過程中形成的地域性遺傳標志,而與病毒的分離年代及mdv的致病型等因素無關。They examined the number of mutations in the dna sequence of each species to estimate its rate of evolutionary change
他們檢查了每個物種的dna脫氧核糖核酸序列中基因突變的數量,並以此估計出該物種的進化速度。It possess high homology with those of ck2 ( casein kinase 2 ) a subunits from various species. it is now well established that protein kinase ck2 is a pleiotropic and abiquitous serine or threonine kinase, which is highly conserved during evolution. we obtained the complete cdna sequence of ck2 a via 5 " race
酪蛋白激酶ck2是目前發現的普遍存在於真核生物中的絲氨酸蘇氨酸型蛋白激酶,進化上具有高度的保守性並參與了多種生理功能的調控,因而克隆它的基因具有重要意義。There has been no detectable change in human dna, brought about by biological evolution, in the ten thousand years of recorded history
在有文字記載的歷史的這一萬年間,在人的dna中不存在由生物進化帶來的可察覺的變化。 (人的脫氧核糖核酸沒有變化。 )Ganansuan antidumping hydrochloric acid manufacture, marketing ; chemical raw materials ( excluding hazardous chemicals ) manufacturing, marketing ; chemical products ( excluding dangerous goods ) wholesale, retail ; approved by the office of the provincial import - export trade business
甘氨酸乙酯鹽酸製造、銷售;精細化工原料(不含危險化學品)製造、銷售;化工產品(不含危險物品)批發、零售;按省經貿廳核定的自營進出口業務。The effective method of nucleation and growth of nanoparticles as applied to synthesize w - and mo - containing polyoxometalates nanoparticles in precursor films by making use of their acidity or oxidative property. the controllable synthesis of nanoparticles can be achieved by increasing synthetic cycle of polyoxometalates. the composition, structure, and properties of the as prepared composite films have been characterized in detail by uv - vis, ftir, x - ray photoelectron spectra, scanning electron microscopy, atomic force microscopy, transmission electron microscopy and cycle voltammograms
利用有效的在前體膜中成核、生長納米粒子的方法,使用keggin型鎢系、鉬系多酸,有目的地利用其酸性,強氧化性,在膜中發生反應,從合成出基於多酸的納米粒子,通過增加多酸的反應循環數,我們可以實現了納米粒子的可控合成,採用uv - vis光譜、 ftir光譜、 x -射線光電子能譜、掃描電子顯微鏡、原子力顯微鏡、透射電子顯微鏡、循環伏安對所制備的納米復合膜進行了組成、結構和性質表徵。A review of the applications and prospects of capillary electrophoresis with electrochemical detection to the analysis of biomolecules such as amino acids, protein, dna and hydrocarbon covering, the years from 1994 to 2004, was presented in this paper with 55 references cited
摘要對近年來毛細管電泳電化學檢測在生物分子(氨基酸、蛋白質、脫氧核糖核酸和糖等)分析中的應用進展作出綜述,展望了電化學檢測在毛細管電泳中的應用前景(引用文獻55篇) 。分享友人