桿狀胞 的英文怎麼說
中文拼音 [gǎnzhuàngbāo]
桿狀胞
英文
ingestion rod-
The bacilliform cell penetrate into interior of the fibre to degrade the cellulose strongly and produced a mass of sticky polysaccharides. after cultured 48 hours, the bacilliform cell ' s surface of sporocytophaga have a great change. at this stage the bacilliform produce a lot of sticky polysaccharides. these sticky polysaccharides associated with the sites where the filter paper was decomposed intensively and form thorns on the surface of the bacillium. at the same time, the filter - paper weight loss is the greatest and decomposing rate is the fastest, so we think that the sticky polysaccharides are produced during the cellulose degradation
培養48小時,桿狀細胞的表面結構發生很大的變化,此時的菌體表面已產生大量的粘性多糖,這些粘性多糖因菌體在纖維素表面滑動而在菌體表面形成突起,即在纖維素被旺盛降解部位的菌體表面產生了大量突起;而產生突起的菌體深入到纖維素分子內部,纖維素表面可以清晰地看到由於菌體嵌入纖維素分子內部而留下的凹陷。The mechanism enhancement of the optical brightener is not known. shapiro et al. postulated that selected brightener including m2r inhibit or alter the chitinous peritrophic membrane ( pm ), creating gaps in the membrane or gut lining and perhaps allowing more virions to pass from the gut lumen into the hemocoel
光增白劑對桿狀病毒的增效作用的機理存在兩種推測一種觀點認為光增白劑是通過破壞圍食膜結構的完整性,促使更多的病毒粒子穿越圍食膜而發動感染的;另一種意見認為光增白劑能延遲中腸上皮細胞的脫落,促進病毒的復制繁殖。The various forms of sclereid include the star - shaped astrosclereid, the rodshaped macrosclereid, and the isodiametric stone cell
石細胞形態各異,有星形的星狀石細胞,桿狀的大石細胞,以及等徑石細胞等。In the thesis, we tried to transfer the novel non - lytic system to suspension culture in spinner flask
另外一方面,有文獻利用桿狀病毒作為載具,將外來基因送入哺乳動物細胞表現,且不會對哺乳動物細胞的生長造成影響。They are also the key components of insect - baculovirus expression system ( bevs ), which has been one of the four main expression systems in gene engineering
同時,桿狀病毒昆蟲細胞表達系統是一類重要的真核表達系統,現已成為目前基因工程四大表達系統之一。( 2 ) the chromosomes of bmn cells showed the typical characteristics of lepidoterati insects. chromosomes of matephase without apparent centromere are short pole - like and pellet - like
( 2 ) bmn細胞的染色體表現為典型的鱗翅目昆蟲的染色體:中期染色體短桿狀或顆粒狀、無明顯的著絲粒。The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時,還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試,並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學,細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞,但其遺傳學和細胞生物學背景知之甚少。In the adherent culture, the virus did not result in cell lysis after the insect cells were infected
而在本論文中,我們嘗試將此一非溶裂昆蟲細胞/桿狀病毒表現系統轉移到旋轉瓶的懸浮培養。Baculovirus / insect cell system has been widely used for recombinant protein production, but traditional system eventually resulted in cell lysis, so that the expressed recombinant protein was lost into medium
摘要:桿狀病毒/昆蟲細胞系統已經被廣泛的應用在重組蛋白質的生產上,但傳統的桿狀病毒感染后會造成細胞溶裂,而使得表現出的重組蛋白質流失到培養基中。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully. recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins. the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2
實驗結果我們成功地構建了滴度高達2x10 『 pm llil的angiostatin重組? 2 ?桿狀病毒,並在昆蟲細胞sffi中高效表達了分子量為53kd的an giostatin重組蛋白,重組angiostatin蛋白不僅在體外顯著抑制內皮細胞的生長, k 。Was supplied. its essential characteristics are as follows : short rods, 2. 0x1. 6 ^ m in diameter, arranged singly, gram negative, facultatively anaerobic, having both a respiratory and a fermentative type of metabolism, oxidase negative and catalase positive
通過形態觀察及生理生化特徵測定發現菌株w12為革蘭氏陰性菌,短桿狀,對數生長期細胞大小為: 2 . 0 1 . 6 m ,單個排列;兼性厭氧,氧化酶陰性,發酵葡萄糖產酸產氣,不運動。The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus
結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中, meq蛋白僅局限於細胞核內,而且隨著感染后( pi )時間的增加,具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。The vegetative cells of the nust03 are rods and the spores are spherical
菌株的營養體細胞桿狀,粘孢子球形。The number and spacing of the rods controls how light reflects which produces different colors
桿狀細胞的數目和間隔決定了如何地反射光,因而產生了不同的顏色。The outer layer of the barbules consists of a two - dimensional crystal framework made of melanin rods connected by keratin ? a fibrous protein ? in a lattice pattern
外層的小羽枝由一個二維的結晶狀結構組成,由黑色素桿狀細胞構成,與角蛋白相連? ?一種纖維狀蛋白質? ?在一種格子式樣圖案裏面。In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system, respectively. in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus. a preliminary bioassay was conducted
第五章利用桿狀病毒bac - to - bac系統構建了含有油桐尺蠖核多角體病毒的類蝸牛毒素基因的重組病毒racbacctl和rhabacctl ,在其相應宿主甜菜夜蛾和棉鈴蟲的細胞水平和蟲體水平進行了超表達實驗。The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock
構建重組病毒:用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞,通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。The recombinant meq - baculovirus was obtained by co - transfecting the insect sf9 cells with pblubac4 - meq and linearised bac - n - blue dna. the recombinant baculovirus was selected by plaque assay and confirmed by pcr technique and sequencing of the inserted gene
應用重組痘病毒表達的meq制備的單抗23b46對重組桿狀病毒感染的sfg細胞及其裂解物分別進行間接免疫熒光試驗、 westemblot和免疫沉澱試驗的檢測。A band neutrophil is seen on the left, and a large, activated lymphocyte on the right
左上是一桿狀核嗜中性粒細胞,右下可見一個大的、已活化的淋巴細胞。 )分享友人