桿狀體 的英文怎麼說

中文拼音 [gǎnzhuàngtǐ]
桿狀體 英文
rhabdite
  • 桿 : 桿名詞（桿子） pole; staff
  • 狀 : Ⅰ名詞1 （形狀） form; shape 2 （情況） state; condition; situation; circumstances 3 （陳述事件或...
  • 體 : 體構詞成分。
  • 桿狀 : rhabditiform桿狀病毒 rhabdovirus; 桿狀細胞 rhabdocyte
  1. The bacilliform cell penetrate into interior of the fibre to degrade the cellulose strongly and produced a mass of sticky polysaccharides. after cultured 48 hours, the bacilliform cell ' s surface of sporocytophaga have a great change. at this stage the bacilliform produce a lot of sticky polysaccharides. these sticky polysaccharides associated with the sites where the filter paper was decomposed intensively and form thorns on the surface of the bacillium. at the same time, the filter - paper weight loss is the greatest and decomposing rate is the fastest, so we think that the sticky polysaccharides are produced during the cellulose degradation

    培養48小時，桿狀細胞的表面結構發生很大的變化，此時的菌體表面已產生大量的粘性多糖，這些粘性多糖因菌體在纖維素表面滑動而在菌體表面形成突起，即在纖維素被旺盛降解部位的菌體表面產生了大量突起；而產生突起的菌體深入到纖維素分子內部，纖維素表面可以清晰地看到由於菌體嵌入纖維素分子內部而留下的凹陷。
  2. Construction of baculovirus transfer vectors for expression of core antigens of newcastle disease virus

    核心抗原重組桿狀病毒轉移載體的構建
  3. ( 2 ) the chromosomes of bmn cells showed the typical characteristics of lepidoterati insects. chromosomes of matephase without apparent centromere are short pole - like and pellet - like

    （ 2 ） bmn細胞的染色體表現為典型的鱗翅目昆蟲的染色體：中期染色體短桿狀或顆粒狀、無明顯的著絲粒。
  4. The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses

    同時，還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試，並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學，細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞，但其遺傳學和細胞生物學背景知之甚少。
  5. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  6. Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully. recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins. the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2

    實驗結果我們成功地構建了滴度高達2x10 『 pm llil的angiostatin重組? 2 ?桿狀病毒，並在昆蟲細胞sffi中高效表達了分子量為53kd的an giostatin重組蛋白，重組angiostatin蛋白不僅在體外顯著抑制內皮細胞的生長， k 。
  7. The rods are about 10, 000 times as sensitive as the cores.

    桿狀體的光敏度大約是心狀體的一萬倍。
  8. Expression e2 gene of classical swine fever in mammalian cell

    2基因的克隆及其桿狀病毒表達載體的構建
  9. The vegetative cells of the nust03 are rods and the spores are spherical

    菌株的營養體細胞桿狀，粘孢子球形。
  10. In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system, respectively. in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus. a preliminary bioassay was conducted

    第五章利用桿狀病毒bac - to - bac系統構建了含有油桐尺蠖核多角體病毒的類蝸牛毒素基因的重組病毒racbacctl和rhabacctl ，在其相應宿主甜菜夜蛾和棉鈴蟲的細胞水平和蟲體水平進行了超表達實驗。
  11. The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock

    構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。
  12. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  13. It has been demonstrated that optical brightener ( or fluorescent brightener ) can enhance viral activity, provide uv protection for nucleopolyhedrovirus ( npv ) and increase host susceptibility

    光增白劑對桿狀病毒具有增效和保護作用，能提高蟲體對桿狀病毒敏感性，這對于擴大桿狀病毒殺蟲劑的應用具有重要的意義。
  14. To obtain large amounts of appa phytase, the appa gene was subcloned into the prokaryotic expression vector pet - 28a ( + ) and baculovirus transfer vector pvl - 1393 under the control of the lac and polyhedrin promoter, respectively. the appa phytase was overexpressed in e. coli strain bl21 induced by lactose

    為了大量表達appa植酸酶，我們將appa基因分別克隆至原核表達載體pet - 28a （ + ）和桿狀病毒轉移載體pvl - 1393中，將其分別置於lac和polyhedrin啟動子控制之下。
  15. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  16. This dissertaion, on the basis of the other studies, on the one hand, researched and analysed the chromosomes and mitoses of the cell line - bmn, which is widely used in the silkworm baculovirus expression system as an engineering cell. on the other hand, the dissertation attempted to explore establishment of the new silkworm embryoni cell line by primary culture

    同時，家蠶體外細胞培養研究的進行，不僅可以為家蠶細胞生物學的基礎理論研究提供良好的研究系統，而且在家蠶資源的開發與利用方面也具有重大的意義。本研究在借鑒前人研究的基礎上，一方面對現在廣泛應用於家蠶桿狀病毒表達系統的工程細胞? bmn細胞的染色體和有絲分裂進行了研究與分析。
  17. The interest gene was inserted in the - tha l. tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system. after analysis by restriction endonuclease and pcr, the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid, the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l

    同時將該目的基因插入到桿狀病毒表達系統的供體質粒pf _ （ ast ） b _ （ ac ） htb的xba 、 xho多克隆位點間，經酶切、 pcr鑒定后，將重組的供體質粒gp1 - fast轉化到含有桿狀病毒和輔助質粒的dh10b _ （ ac ）感受態細胞中，獲得了表達gpvh1株vp1的重組桿狀病毒gp1 - bac 。
  18. Baculovirus is a kind of double - stranded circle large dna virus, which has been developed as an environment sound pesticide and a powerful vector of foreign protein expression as well as a potential vector of gene therapy

    桿狀病毒是一類雙鏈環狀dna病毒，作為生物防治殺蟲劑及高效外源蛋白表達載體得到廣泛研究。近來有許多學者致力於桿狀病毒作為基因治療載體的研究。
  19. Adopt at present most advanced large - scale super sap finite element structure analyse procedure to go on numerical value calculation for model of drilling rod, drilling rod whole stress simulation of state that receive show and reflect drilling rod various kinds of stresses under different working states distribute the characteristic clearly, make stress state of drilling rod reach visual, make people able to understand informations of drilling rod of stress status in depth too at the same time, so that the design of the improvement drilling rod constantly is in order to meet the demands of actual project

    採用當前最先進的大型supersap有限元結構分析程序對鉆桿的模型進行了數值計算，得到的鉆桿整體應力狀態的模擬顯示清楚地反映了鉆桿在不同工作狀態下的各種應力分佈特徵，同時也使鉆桿的應力狀態達到了可視化，使人們可以深入了解鉆桿應力狀態信息，以便不斷的改進鉆桿的設計以滿足實際工程的需要。
  20. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
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