步黴素 的英文怎麼說

中文拼音 [bùméisù]
步黴素 英文
pasomycin
  • 步 : Ⅰ名詞1 （步度; 腳步） pace; step 2 （階段） stage; step 3 （地步; 境地） condition; situation; st...
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  • 黴素 : mycin
  1. Furthermore, it produced 2 unusual components d24 - 1 and d24 - 2. it was basically confirmed, by hplc and ms, that d 24 - 1 was oligomycin a ; while d24 - 2 was 5 - oxo - avermectin la

    此外，該菌株還產生兩個異常的組分d24和d242 ，經hplc及質譜進一步分析，初步確定異常組分d241為寡黴素a ，而d24 2為5
  2. In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated

    將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活，導致發酵產物中4個a組分（不需要的組分）的消失；將基因簇中的avec基因通過同樣手段，使其失活，導致發酵產物中4個「 1 」組分的消失，而主要積累「 2 」組分（進一步改造可成為伊維菌素的前體b _ 2組分） 。
  3. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  4. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  5. The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga, 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm, this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step

    Western印跡分析表明對于菌株dh5 pkkfpga ， 5 - 10的原前體青黴g素酰化酶在胞內形成了包涵體，說明其成熟的限速步驟在胞內的運輸階段，而菌株dh5 psmlfpga則無明顯包涵體形成，說明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表達能力高於菌株dh5 pkkfpga 。
  6. Abstract : study and application of red koji, monascus, monascus pigment and pharmacological activity of fermented product by monascus were reviewed. several different fields which should be further studied on red koji and monascus were pointed out

    文摘:綜述了紅曲、紅曲色素和紅麴黴發酵產物藥理作用的研究和應用的最新成果，提出了進一步的研究方向。
  7. Primary observation of reversal of multidrug resistance in k562 a02 cells with radix angelicae sinensis

    02細胞對阿黴素耐藥性的初步觀察
  8. Using colophony - paraffin ( cp ) embedding tissue section technique and immunohistochemical streptalidin - peroxidase ( sp ) method, we have investigated the distribution of 5 - ht neurons in the brain of camponotus japonicus ( big worker ant ), compared the immunoreactivity with gaba, and primarily discussed the character of 5 - ht distribution. the results show that 5 - ht immunoreactive processes originate from a relatively small number of cell bodies but each neuron has processes over a large volume of the neuropil of the brain

    本實驗採用樹脂石蠟（ cp ）組織包埋切片技術和鏈黴菌抗生物素蛋白-過氧化物酶（ sp ）免疫組織化學方法，研究了5 - ht能神經元在日本弓背蟻（ camponotusjaponicus ）大工蟻腦中的分佈情況，並與gaba的分佈進行了比較，初步探討了5 - ht在各個腦區的分佈規律和特點。
  9. In this study, we examined class 1 and 2 integron in shigella sonnei and shigella flexneri isolates from hangzhou, china, to investigate their distribution and determine their gene cassette arrays and their location in genome. a atypical class 1 integron without 3 " conserved region was found to prevail among shigella flexneri isolates, and the excision models of it ' s gene cassette were charactered by inverse pcr. moreover the roles of gene cassettes in the atypical class 1 integron and class2 integron to confer resistance were discussed

    本研究對杭州地區近年分離的福氏和宋內氏志賀菌株進行類和類整合子的檢測，了解其分佈特徵；進一步通過克隆測序和southenblot ，確定其攜帶的基因盒組成特徵以及其在基因組中的位置；在研究中發現了一種缺少3 』保守區的非典型類整合子，通過反轉pcr了解此整合子的切除基因盒的特徵；並對非典型類整合子和類整合子各自攜帶的aada賦予宿主菌鏈黴素抗性的不同能力，進行了探討。
  10. Plasmid psyxl, containing a 12kb insert of s. tenebrarius dna, was isolated from one of six colonies. apramycin resistant gene was located in 1. 5 - kb sphl - kpnl fragment of plasmid psyxl though further studies

    陽性克隆中重組質粒psyx1含有12kb黑暗鏈黴菌的dna ，對12kb片段進一步分析將安普黴素抗性基因定位在1 . 5kbsphi - kpni片段上。
  11. 70kb and 130kb in size respectively. though pks gene cluster for antibiotics fr - 008 was cloned, the genes for amino - mycosamine residue remained unknown. thus, attempt was also made to clone the desired gene ( s ), streptomyces sp

    首先從大量的發酵液中提取分離了鏈黴菌fr - 008產生的抗生素fr - 008 ，並通過大孔樹脂吸附、柱層析等手段進行了初步提純。
  12. On aflatoxin, further evaluation of the asta method for testing this parameter is being undertaken

    對于使用asta法來檢驗黃麴黴毒素參數的方法正在進行進一步評估。
  13. Positive transformants were also detected by kan resistant re - screening on solid medium or directly detection in field and southern blot

    利用卡那黴素抗性再篩選及kan抗性大田直接篩選、 southernblot等方法對陽性植株作了進一步檢測。
  14. The effect of some factors, including the appropriate materials for isolating protoplasts, the concentrations of enzyme, period of digestion and temperatures, and osmotic pressure stabilizers on the isolation and regeneration of protoplasts in penicillium digitatum were studied. the results demonstrated that the purified protoplasts could regenerate through double layers of czapek medium containing 0. 7mol / l nacl. the regeneration rate could reach 24. 9 %

    通過對制備材料、酶液濃度、酶解時間、酶解溫度、滲透壓穩定劑的種類和濃度等因素的實驗研究，得到了一套制備指狀青黴（ penicilliumdigitatum ）原生質體的有效方法，並在雙層培養基上初步實現了原生質體的再生，再生率可達24 . 9 。
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