法比亞內克 的英文怎麼說
中文拼音 [fǎbǐyǎnèikè]
法比亞內克
英文
fabianek- 法 : Ⅰ名詞1 (由國家制定或認可的行為規則的總稱) law 2 (方法; 方式) way; method; mode; means 3 (標...
- 比 : Ⅰ動詞1 (比較; 較量高下、 長短、距離、好壞等) compare; compete; contrast; match; emulate 2 (比...
- 內 : 名詞1. (內部; 里頭; 里邊) inner; inside; within 2. (妻子或妻子的親屬) one's wife or her relatives 3. (姓氏) a surname
- 克 : 克i 動詞1 (能) can; be able to 2 (克服; 克制) restrain; control 3 (攻下據點; 戰勝) overcome...
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These countries purchased us and canadian dollars, pounds sterling, deutsche mark, belgian and french francs, australian dollars, mexican pesos, argentine pesos, italian lire, danish and norwegian kroner, swedish kronor, japanese yen, austrian schillings, spanish pesetas, netherlands guilders, finnish mark, irish pounds, south african rand, brazilian cruzeiros, venezuelan bolivars and malaysian ringgits, aggregating $ 22008 million, in exchange for an equivalent amount of their own currencies
這些國家購買美元、加拿大元、英鎊、德國馬克、比利時法郎和法國法郎、澳大利亞元、墨西哥比索、阿根廷比索、義大利里拉、丹麥克郎和挪威克郎、瑞典克郎、日元、奧地利先令、西班牙比塞塔、荷蘭盾、芬蘭馬克、愛爾蘭鎊、南非蘭特、巴西克魯賽羅、委內瑞拉博利瓦和馬來西亞林吉特,兌換成各成員國本國的等值貨幣,總金額達220 . 08億美元。These countries purchased us and canadian dollars, pounds sterling, deutsche mark, belgian and french francs, australian dollars, mexican pesos, argentine pesos, italian lire, danish and norwegian kroner, swedish kronor, japanese yen, austrian schillings, anish pesetas, netherlands guilders, fi ish mark, irish pounds, south african rand, brazilian cruzeiros, venezuelan bolivars and malaysian ringgits, aggregating $ 22008 million, in exchange for an equivalent amount of their own currencies
這些國家購買美元、加拿大元、英鎊、德國馬克、比利時法郎和法國法郎、澳大利亞元、墨西哥比索、阿根廷比索、義大利里拉、丹麥克郎和挪威克郎、瑞典克郎、日元、奧地利先令、西班牙比塞塔、荷蘭盾、芬蘭馬克、愛爾蘭鎊、南非蘭特、巴西克魯賽羅、委內瑞拉博利瓦和馬來西亞林吉特,兌換成各成員國本國的等值貨幣,總金額達220 . 08億美元。Although we have performed in many places like genk belgium, holstebro denmark, genosa, milano italy, and now we return to holstebro denmark, we decided to write these words to supreme master ching hai before becoming busier in the coming months for we will travel to london england, april, paris france, may, molin australia, june budapest hungary, july, fribourg switzerland, august, brussels belgium, september, firenze italy, october, sao paulo, rio de janeiro brazil, november, etc
在這段時間我們在很多地方演出,像是健克比利時,侯斯特伯丹麥,吉諾沙米蘭義大利,現在我們回到了侯斯特伯丹麥。下幾個月將會更忙,我們決定在此前夕,給您寫這封信。之後我們就要去倫敦英國,四月,巴黎法國,五月,毛林澳大利亞,六月,布達佩斯匈牙利,七月,菲柏格瑞士,八月,布魯塞爾比利時,九月,費然斯義大利,十月,聖保羅里約熱內盧巴西,十一月等等。On the republican side, arizona senator john mccain has a clear lead nationwide and no one expects his chief ravel, former arkansas mike huckabee, to win the nomination, no matter how well he does in wisconsin
在共和黨那邊,亞利桑那州參議員約翰?麥凱恩明顯在全國范圍內得到較高的支出率,人們認為他的主要競爭對手,前阿肯色州州長麥克?哈克比,不論他在威斯康辛州表現多麼好,都無法獲得提名。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。分享友人