Dna damages caused by so2 and lead acetate were studied with the single cell microgel electrophoresis technique ( or comet assay ) in order to confirm the damaging degree of lead ( as an important component of atmosphere particle matter ) on dna from male mice exposed to so2. the migrating distances of dna of brain, lung, spleen and kidney cells of mice increased significantly, compared to the control group under conditions of single and combined poisoning of so2 ( 42mg / m3 ) and lead acetate ( 0. 2 % ), and lead could strengthen dna damage degree by so2 in nuclear dna of brain, kidney, spleen cells. damaging degree of so2 on nuclear dna of lung cell of mice was more severe than that of lead
為了明確大氣顆粒物中的重要組分? ?鉛在二氧化硫所致dna損傷中的作用程度,利用單細胞凝膠電
泳技術( singlecellgelelectrophoresis , scge ,或稱彗星實驗, cometassay )研究了鉛與二氧化硫的聯合污染,結果表明在42mg m ~ 3so _ 2和0 . 2醋酸摘要一abstract鉛單獨及聯合染毒條件下,小
鼠腦、肺、腎、脾細胞dna遷移距離均比對照顯著增加;鉛加劇了50 :對腦、腎、脾細胞核dna的損傷程度; 50 :對肺細胞核dna的損傷程度要比鉛的損傷大,小
鼠肺細胞核dna遷移距離在50 :和醋酸鉛聯合作用組與醋酸鉛單獨作用組間有極顯著性差異( p < 0 . 01 ) ,而與502單獨作用組間沒有顯著性差異。
We present a preliminary study on the zymograms of esterase ( est ), lactate dehydrogenase ( ldh ), malate dehydrogenase ( mdh ) from four groups of microtus fortis by vertical discontinous polyacrylamide gel electrophoresis method. the number, mobility and dye intensity of the enzyme band were analyzed
採用垂直板不連續聚丙烯酰胺凝膠電
泳法對四類東方田
鼠的酯酶( est ) 、蘋果酸脫氫酶( mdh )和乳酸脫氫酶( ldh )進行了初步研究。
Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電
泳和免疫印跡的方法,用哺乳動物大
鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定,結果表明:抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應,同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在,表明在植物中有類三磷酸肌醇受體蛋白的存在;用免疫膠體金方法,發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。
However according to the view of acoustics, click includes mixed frequencies, so further studies are necessary to investigate the physiological significance of amygdaloid modulating effect on the ascending auditory information at the cortical level. in this paper we firstly observe the characteristics of the acoustic response of neurons in a - i evoked by pure tone ; then investigate the influence of la stimulation on the acoustic response of these neurons and the physiological significance of such influence ; revea l the neural pathway mediating this effect with the neurohistological method ; and study the neurotransmitter and its receptor participating in this effect
本文採用電生理學方法考察大
鼠皮層a區神經元純音反應特徵,觀察杏仁外側核( la )對a區神經元聲反應的影響,運用神經組織學方法揭示介導這一影響的神經環路,採用多管微電極記錄結合微電
泳技術的方法研究參與這種影響的神經遞質及受體,進一步探討了這種調制性影響的生理學意義。
The result showed that sea cucumber polypeptide has no obvious effect on body weight in mice, significantly prolongs the time of loaded - swimming and rolling stick, stringly degrades the content of blood urea nitrogen and increases the content of hepatic glycogen of postexercise mice
結果表明,低分子量海參肽對小
鼠體重無顯著影響,但能明顯延長小
鼠的負重遊
泳時間和轉棒時間,顯著降低運動后小
鼠的血尿素氮含量,同時提高了肝糖原含量。
Extensive mitochondrial dna polymorphisms were found among lagurus lagurus, mus musculu , rattus norvegicus and mice. the findings will help us to understand the dispersion and evolution of these animals
通過瓊脂糖凝膠電
泳對這些片段進行測定,同時估算出草原兔尾
鼠線粒體dna的長度約為16 . 6kb 。
We also investigated the pathological changes of mouse liver, thymus and cerebrum cortex challenged by so2 inhalation by in vivo tests. we studied the apoptotic induction on mouse spleen cells and cytotoxicity of human embryo lung fibroblasts of so2 derivatives by in vitro tests. in vivo tests of sulfur dioxide inhalation showed : ( 1 ) effects on mouse lung of so2 challenge : we found no significant apoptotic changes induced by so2 inhalation but obvious pathological changes of lung with vacuolating of osmiophilic multilamellar bodies which maybe related with the decrease of surfacant and decrease of microvillus of type ii alveolar cells ; we also found thickening of part of basement lamina between type i alveolar cells and capillary endothelium cells which may inhibit the dispersion of oxygen and contribute to lung dysfunction
二氧化硫熏氣染毒的體內實驗結果表明,在本次實驗的濃度范圍內( 56mg m ~ 3 、 112mg m ~ 3 、 168mg m ~ 3低、中、高三個濃度) : ( 1 )通過透射電鏡、 dna凝膠電
泳分析和流式細胞分析發現二氧化硫吸入染毒一周對小
鼠肺臟沒有明顯的凋亡誘導作用,但通過透射電鏡觀察發現二氧化硫可引起肺臟明顯的超微結構改變,引起型肺泡上皮細胞板層體空泡化,微絨毛減少,線粒體緻密化或腫脹變性;肺泡血管內皮細胞和型肺泡上皮細胞之間基膜增厚,使氧氣彌散功能出現障礙,從而降低肺功能。
Sh chou 、 t kno 、 m lin 、 ok lee , " in utero transplantation of human bone marrow - derived multipotent mesenchymal stem cells in mice. " , journal of orthopaedic research , vol. 24 , no. 3 , march 2006 , pp. 301 - 312
方晴、周秀慧、柳如宗、林震煌,毛細管電
泳/線上掃集濃縮技術對老
鼠血液中麥角乙二胺的分析與研究,第63卷,第1期, 2005年3月,頁77 - 86 。
In this article, we microinjected camp ( as activator of pka ) and protein kinase inhibitor ( pki ) ( as inhibitor of pka ) into mouse 1 - cell stage fertilized eggs, the camp concentration, pka and mpf activaty were detected, also the cdc25c, cdc2 phosphorylated state and the concentration of ptyr15 for cdc2, cyclin b1. materials females of 4 - 5 week - old kuming mice and males of 8 week - old kuming mice were supplied from the department of laboratory animals, china medical university
本實驗應用pka激動劑camp及抑制劑pki顯微注射入小
鼠二一細胞期受精卵並觀察卵細胞m期形態學變化及pka對mpf活性的影響以及cdc25c , cdcz電
泳遷移率, cdcz的磷酸酪氨酸ptyrl及周期素b含量,為揭示pka在哺乳動物細胞周期調控機制,對生長、發育、癌變、死亡的研究提供理論依據。
Method : dividing 90 kunming mice to 3 groups, observing the index of weight loading swimming test, bearing hypoxia at normal pressure, activity of lactic dehydrogenase
方法: 90隻雄性昆明種小
鼠分為3組,觀察艾灸后小
鼠的負重遊
泳試驗、常壓耐缺氧時間、乳酸脫氫酶活力等指標。
Result : moxibustion had the function of reinforcing weight loading swimming ability and activity of lactic dehydrogenase and lengthening the time of bearing hypoxia at normal pressure
結果:艾灸能有效地提高小
鼠的負重遊
泳能力和常壓耐缺氧的時間,並可增強乳酸脫氫酶的活力。
A gentleman, an idealist, an irregular church visitor, a 9 to 5 job, a computer geek, a condo at riverside, an old lexus, a mortgage, a hamster ( sorry, she escaped recently ), a regular swimmer, a enthusiastic reader, a traditional music lover, a non - smoker, a red wine promoter, an average cook, a disastrous bargainer, a easy going teammate, a fantastic listener
一位紳士,一個理想主義者,一個有時去教堂的人,一份9 - 5的工作,一個電腦歪才,一套在河邊的單元房(高層) ,一部老爺車(凌志) ,一份按揭,一個小倉
鼠(對不起,她最近逃跑了) ,一個游
泳池的常客,一位熱情的讀者,一位傳統音樂的聽眾,一個不吸煙的人,一個紅葡萄酒的愛好者,一個不太好的廚子,一個經常在討價還價中吃虧的人,一個容易相處的隊友,一位耐心的傾聽者。
A gentleman, an idealist, an irregular church visitor, a 9 to 5 job, a computer geek, a condo at riverside, an old lexus, a mortgage, a hamster ( sorry, she escaped recently ), a regular swimmer, a enthusiastic reader, a traditional music lover, a non - smoker, a red wine advocate, an average cook, a disastrous bargainer, a easy going teammate, a fantastic listener
一位紳士,一個理想主義者,一個有時去教堂的人,一份9 - 5的工作,一個電腦歪才,一套在河邊的公寓(高層) ,一部老爺車(凌志) ,一份按揭,一個小倉
鼠(對不起,她最近逃跑了) ,一個游
泳池的常客,一位熱情的讀者,一位傳統音樂的聽眾,一個不吸煙的人,一個紅葡萄酒的愛好者,一個不太好的廚子,一個經常在討價還價中吃虧的人,一個容易相處的隊友,一位耐心的傾聽者。
Results yj - e could decrease the duration of immobility in the tst and fst and showed potent antidepressant effect, but yj - w has no obvious effects
結果越鞠丸醇提物能不同程度地縮短小
鼠懸尾不動時間和小
鼠強迫游
泳不動時間,具有較強的抗抑鬱作用;而水提物活性不明顯。
Methods 2 behavioral depression animal models ( the tail suspension test, tst and the forced swimming test of mice, fst ) were used to evaluate antidepressant activities of the ethanol extract ( yj - e ) and the water extract ( yj - w )
方法採用小
鼠懸尾實驗和小
鼠強迫游
泳試驗兩種行為絕望
鼠抑鬱模型,對越鞠丸醇提物和水提物的抗抑鬱作用進行比較。
In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小
鼠mbd3基因145 ? 169bpcdna序列合成探針,經[ - ~ ( 32 ) p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達,同時分析了mbd3基因誘導表達的組織特異性,劑效和時效關系;結合mbd3基因啟動子區序列分析,以- 164 ? - 179bp雙鏈dna序列合成探針,經[ - ~ ( 32 ) p ] dctp標記后通過電
泳遷移率改變實驗( emsa )和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
The treatment effect of guishen granule on experimental type diabetes in rats
脂糖舒對正常和糖尿病小鼠缺氧和游泳耐力的影響