海藻酸合酶 的英文怎麼說
中文拼音 [hǎizǎosuāngě]
海藻酸合酶
英文
alginate synthase-
Experiment shows that tpsl gene can endow organism the ability of synthesis trehalose, the dephosphorylation of the trehalose - 6 - phosphate is not special, and it can be replaced by other phosphatases. the tpsl gene from saccharomyces cerevisiae was cloned by pcr amplification
實驗證實tps1基因就可以使生物體獲得產生海藻糖的能力,酵母的海藻糖合成酶復合體中6 -磷酸-海藻糖的脫磷酸化作用是非特異性的,它可由生物體內的其它酯酶所代替。Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
Pcr產物經回收后,經瓊脂糖凝膠電泳鑒定后,與pcambia1303連接並轉化大腸桿菌dh5a ,陽性重組子經pcr和限制性內切酶酶切圖譜分析,表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。The tpsl gene was introguced into immature embryo cells of 12 - 16 days wheat cultivars wen. 6 via gene gun delivery technique. resistant calli were selected on medium containing hygromin ( 40 - 60mg / l )
用基因槍將海藻糖- 6 -磷酸合成酶基因( tps1 )導入小麥栽培品種溫6的12 - 16日齡的幼胚細胞中。Using the tpsl gene digested form the prokaryotic expressing vector as template, according to the published sequence, pcr primers of tpsl gene was designed, 1500bp fragment of tpsl was generated
以從t - vector中酶切得到的tps1為模板,根據已知序列設計pcr引物,擴增海藻糖- 6 -磷酸合成酶基因( tps1 ) 。Tps1 is a functional trehalose - 6 - phosphate synthase that converts udp - glucose and glucose - 6 - phosphate to trehalose - 6 - phosphate, and tps2 encodes the phosphatase, which dephosphorylates trehalose - 6 - phosphate, and tsl1 together with tps3 is supposed to stabilize and regulate the complex
Tps1基因編碼udp -葡萄糖依賴性的海藻糖- 6 -磷酸合酶, tps2基因編碼海藻糖- 6 -磷酸酯酶, tps3和tsl1兩個基因的產物可能對復合體起穩定和調節作用。分享友人