測序膠 的英文怎麼說
中文拼音 [cèxùjiāo]
測序膠
英文
sequencing gel-
Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4
Sds page分析得到3條主要蛋白帶,剪下這三條帶進行膠內賴氨酸內切酶的消化,通過高效液相色譜分離肽段,選擇性進行肽段的氨基酸序列測定。The anion surfactant nals was the most efficient in the process and was selected as collector by orthogonal method. the interfacial tension and cmc of nals ( cmc 6. 5 10 - 3m ) in the solution was measured by hanging ring test, the function relationship between interfacial tensions and nals concentration was : = - 0. 9523 in 2c - 24. 534 in c - 81. 411. the relation of interfacial adsorption equilibrium in the solution system was respectively tested and calculated with gibbs equation and dynamic method. the conclusion showed that ( 1 ) the experiment result of dynamic method with was highly in coordination with the value which from theoretical calculation. ( 2 ) the surfactant molecule was in arrangement of monomolecular layer at the gas - liquid interface
用吊環法測定了十二烷基硫酸鈉水溶液不同濃度與其界面張力的關系,擬合得到的回歸方程為= - 0 . 9523ln ~ 2c - 24 . 534lnc - 81 . 411 ,同時還測得其臨界膠束濃度值( cmc為6 . 5 10 ~ ( - 3 ) m ) ,運用吉布斯等溫方程和動態法分別計算和測定了該溶液系統的界面吸附平衡關系,結論表明: ( 1 )動態法測定結果基本吻合吉布斯方程的理論計算; ( 2 )表面活性劑分子在氣?液兩相界面是以單分子層形式定向有序排列的。The 2nd pair of primers was designed according to the sequence of strain th - 98 collected in genbank. concentration of tp was analyzed after amplification invitro by rt - pcr, purified by low melt agarose and labeled by digoxigenin
根據genbankth - 98株序列設計第2對引物,應用rt - pcr方法體外擴增該片段(命名為tp ) ,瓊脂糖凝膠純化后測定其濃度和純度,進行非放射性地高辛標記。The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned
從陽性克隆子中提取克隆片段,經序列測定分析,結果表明,克隆片段的cdna大小為816bp ,編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank , embl , ddbj , pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索,結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ;蛋白質氨基酸序列的同源率為90 95 ,表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4The temperature from amorphous to crystal of tungsten oxide sol - gel films with catalyst is increased and the reason is in studying. as results of tunnel scan - afm, both pt sputtered tungsten oxide films and pt sputtered tungsten oxide sol - gel films there is distinct and out - of - order parallel line structure on the surface of amorphous. molecules of the sample tend to tetrahedron and the former has more planarer structure
隧道-原子力顯微鏡測試結果表明:非晶態時,磁控濺射摻鉑薄膜樣品表面和溶膠凝膠摻鉑樣品表面都有明顯的平行線狀結構,長程無序,分子趨於四面體結構,只是前者比後者表面較平整;晶態時,磁控摻鉑樣品在自然生長面上原子呈平面分佈,長程有序,溶膠摻鉑樣品則呈wo6面心結構。In this dissertation, sequences of rdna from eleven important phytoplanktonic strains collected at jiaozhou bay are amplified, cloned, sequenced and compared, and the phylogenetic relationships among them are analyzed using the ssu rdna sequences
本論文首先對分離自膠州灣的十一種浮游植物的rdna序列進行了pcr擴增、克隆、序列測定、序列相似性分析,並利用測得的小亞基( ssu ) rdna序列分析了這些浮游植物的系統進化關系。Adhesives. short term method for measuring the emission properties of low - solvent or solvent - free adhesives after application. general procedure
膠粘劑.測量低溶解劑或無溶解劑的膠粘劑在使用後排放特性的短期方法.一般程序Indentificatiort is also the first step towards studies on protein co - and post - translational modification, and ultimately, function. in the present study, the total proteins of the photo - thermo sensitive genie male - sterile rice ( oryza sativa, peiai64s ) spikelet at meiosis stage were used as the material. by optimizing crucial factors and procedures such as sample treatment, electrophoresis parameters, and gel concentration, 2 - d maps with high quality and reproducibility were obtained
用兩種方法對經雙向電泳分離的凝膠上的蛋白質點進行了初步鑒定,一是通過電印跡轉移把蛋白質轉到pvdf膜上,再用edman降解的方法測得部分相對分子質量在10000 - 30000da的蛋白質點的n -端序列,通過網上搜索其同源性對其進行鑒定,並確定該點在凝膠上的位置。The machine which is used for underied - glue lable sticking on cylinder with diameter of 3596mm, can finished setting and colleting bottles at the same time. it s light - optic checking and in - step - with tracing means make sure that the speed of label - sticking can follow the trail of that of bottle - rolling and the labels can be sticked smoothly
該機適用於35 - 96mm的圓柱形的不幹膠標簽的貼標,可一次完成放瓶及收瓶程序,採用光電檢測,同步追蹤,準確保證出標速度與卷瓶速度同步,標簽貼壓平整。Phaa, phab and phac were amplifed from the subclone of pseudomanas sp. producing pha by pcr. the gel electrophoresis analysis showed that the molecular weights of cloned phaa, phab and phac were equal to fragment speculated from three orfs
利用所提取的開放閱讀框架的序列設計三對引物,採用pcr技術,從合成pha的亞克隆片段中分離出phaa 、 phab和phac三個基因片段,經凝膠電泳分析表明,所克隆的三個基因分子量大小與推測的三個開放閱讀框架中基因片段大小一致。Film dosimetry using a filter analysis procedure for radiation protection monitoring
射線防護監控過濾器分析程序使用的膠片劑量測定法Yamabun electronics designs and manufactures thickness measuring systems for use in sheet film production processes
設計和製造單張膠片製造工序中使用的測量裝置。Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method
首先,以成體牛蛙的腦垂體總rna為模板,進行rt ? pcr擴增得到約660bp的特異性pcr產物帶,切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收,將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后,進行菌落pcr 、質粒酶切鑒定,篩選出陽性菌株,測序結果經blast分析,與已報道的牛蛙生長激素基因高度同源,證實此陽性克隆為牛蛙生長激素基因轉化子,命名為pbfgh 。It gives information on working standards for bioaerosol measurements worked out and commonly used in germany within the frame of european guidelines for bioaerosol measurements in the workplace atmosphere
內容描述德國于歐盟體系下量測工作場所中生物性氣膠之詳細標準作業程序。8 computer program, then used to calculate di stance matrix with kimura ' s two - parameter method and construct phylogenetic trees by applying neighbor - joining and maximum parsimony analyses using mega2. 1 computer program. bootstrap analyses were carried out to evaluate statistical reliability based on 500 resamplings of the data set
新鮮或硅膠乾燥樣品被用於總dna提取並用於pcr反應, pcr擴增產物回收后直接用於測序反應,序列數據經chstalx1 . 8軟體排序后,採用mega2 . 1軟體根據kimura 』 s雙因子法計算遺傳距離,並應用鄰接法( neighbour - jointing )和最大簡約法( maximumparsimony )構建系統樹, bootstrap法( 500次重復)評估分支可信度。分享友人