測序凝膠 的英文怎麼說
中文拼音 [cèxùníngjiāo]
測序凝膠
英文
sequencing gel-
The 2nd pair of primers was designed according to the sequence of strain th - 98 collected in genbank. concentration of tp was analyzed after amplification invitro by rt - pcr, purified by low melt agarose and labeled by digoxigenin
根據genbankth - 98株序列設計第2對引物，應用rt - pcr方法體外擴增該片段（命名為tp ） ，瓊脂糖凝膠純化后測定其濃度和純度，進行非放射性地高辛標記。The temperature from amorphous to crystal of tungsten oxide sol - gel films with catalyst is increased and the reason is in studying. as results of tunnel scan - afm, both pt sputtered tungsten oxide films and pt sputtered tungsten oxide sol - gel films there is distinct and out - of - order parallel line structure on the surface of amorphous. molecules of the sample tend to tetrahedron and the former has more planarer structure
隧道-原子力顯微鏡測試結果表明:非晶態時，磁控濺射摻鉑薄膜樣品表面和溶膠凝膠摻鉑樣品表面都有明顯的平行線狀結構，長程無序，分子趨於四面體結構，只是前者比後者表面較平整;晶態時，磁控摻鉑樣品在自然生長面上原子呈平面分佈，長程有序，溶膠摻鉑樣品則呈wo6面心結構。Indentificatiort is also the first step towards studies on protein co - and post - translational modification, and ultimately, function. in the present study, the total proteins of the photo - thermo sensitive genie male - sterile rice ( oryza sativa, peiai64s ) spikelet at meiosis stage were used as the material. by optimizing crucial factors and procedures such as sample treatment, electrophoresis parameters, and gel concentration, 2 - d maps with high quality and reproducibility were obtained
用兩種方法對經雙向電泳分離的凝膠上的蛋白質點進行了初步鑒定，一是通過電印跡轉移把蛋白質轉到pvdf膜上，再用edman降解的方法測得部分相對分子質量在10000 - 30000da的蛋白質點的n -端序列，通過網上搜索其同源性對其進行鑒定，並確定該點在凝膠上的位置。Phaa, phab and phac were amplifed from the subclone of pseudomanas sp. producing pha by pcr. the gel electrophoresis analysis showed that the molecular weights of cloned phaa, phab and phac were equal to fragment speculated from three orfs
利用所提取的開放閱讀框架的序列設計三對引物，採用pcr技術，從合成pha的亞克隆片段中分離出phaa 、 phab和phac三個基因片段，經凝膠電泳分析表明，所克隆的三個基因分子量大小與推測的三個開放閱讀框架中基因片段大小一致。Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method
首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。分享友人