熒光抗原 的英文怎麼說
中文拼音 [yíngguāngkàngyuán]
熒光抗原
英文
fluorescent antigen- 熒 : 形容詞[書面語]1. (光亮微弱的樣子) glimmering 2. (眼光迷亂; 疑惑) dazzled; perplexed
- 光 : Ⅰ名詞1 (照耀在物體上、使人能看見物體的一種物質) light; ray 2 (景物) scenery 3 (光彩; 榮譽) ...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 原 : Ⅰ形容詞1 (最初的; 原來的) primary; original; former 2 (沒有加工的) unprocessed; raw Ⅱ動詞(原...
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A rabbit was infected with a cloned yntatl, blood was collecting from from the rabbit every 3 days after infection within 30 days, 10 clonal trypanosome populations were gotten, infecting a new rabbit by the last non - cloned trypanosome population. repeated above all, thus infected 5 rabbits sequentially. twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia )
用伊氏錐蟲雲南水牛單克隆株yntat1感染兔,感染后30天內,每3天從兔血中分離錐蟲並單蟲克隆,最後一個未單蟲克隆的蟲株感染另一隻兔,重復以上操作,這樣順序感染5隻兔子,共獲得50個單克隆錐蟲種群( tp ) ,經間接免疫熒光和abc酶標試驗鑒定共為20個抗原性互不相同的抗原變異體( vats ) 。Anti - if - protein antibodies were used as probes for immuno - fluorescence, and the reactions of them with different parts of the cell were detected, which suggested the possibility of the existence of the intermediate - like filaments in the cytoplasm. those proteins homologous to the antibodies distributed regularly in the protoplasm. to characterize the corresponding proteins, sds - page and immunoblots were utilized. the 21, 23, 33 and 68kd proteins were distinguished among the diverse protein constituents of the cell. some of these proteins also showed the cross - reactivities with anti - if - proteins antibodies derived from higher organisms. these two evidences both contributed to the homology of some proteins in
以抗中間纖維蛋白抗體作為探針進行免疫熒光實驗,得到細胞內不同部位的陽性反應,暗示原生質中可能存在類中間纖維。這些同源蛋白的胞內分佈具有一定的規律性。進一步採用sds - page和免疫印跡技術研究它們的生化性質,發現4種主要蛋白明顯有別于胞內其他蛋白組分。Throughout the culture period, most cells within the colonies continued to be alkaline phosphata se positive and specific stage embryonic antigen ( ssea - 1 ) positive which has been used routinely to characterize embryonic stem and pgcs cells
經過4 7天培養, pgcs形成典型的鳥巢狀集落。集落在傳代過程中保持堿性磷酸酶活性,胚胎階段性特異抗原1 ( ssea - 1 )免疫熒光染色顯示陽性。Stable fluorescent labels bhhct - eu3 +, bpta - tb3 ", and europium nanoparticle were used to develop sandwich - type time - resolved fluoroimmunoassays for hbsag. fluorescence intensity was directly detected on the surface of the solid phase
利用穩定的稀土標記物bhhct - eu3 + 、 bpta - tb3 +和銪納米顆粒建立夾心型固相時間分辨熒光免疫分析( trfia )直接定量測定乙肝表面抗原( hbsag )的新方法。In this paper, wheat cultivars lovrin 10 ( resistant ) and 5389 ( susceptible ) were selected as materials in this system. the mesophyll protoplasts of wheats ( mpw ) were isolated using cellulase and pectolase digestion. by the indirect immune fluorescent labeling, microtubules ( mts ) pattern in mpw were showed clearly under the confocal laser scanning microscope ( clsm )
本試驗以抗(洛夫林10 ) 、感( 5389 )不同的兩小麥( triticumaestivuml . )品種為材料,採用酶解法制備小麥葉肉原生質體,利用間接免疫熒光方法結合激光共聚焦掃描顯微技術對小麥葉肉細胞原生質體的微管骨架進行了清晰的標記,並探索了微管骨架標記的影響因素。. from the direct mutant of spirulina platensis ( sp - d ), we got high purity and activity phycobiliprotein which could grow crystals. the algae fluorescent probe prepared by coupling the above polyclonal antibody to phycobilipotein not only keeps the property of stronger anti - fluorescence quenching but also has the lower fluorescent background when it was used for labeling stoma cells of pea tendril
以原核表達的peac1為抗原制備了免疫活性較好的抗豌豆肌動蛋白的多克隆抗體,從螺旋藻中純化了高純度、高活性、能結晶的藻膽蛋白,將兩者偶聯制備的藻熒光探針,不僅保持了藻膽蛋白很強的抗熒光淬滅能力,而且用於豌豆卷須氣孔細胞熒光標記時有更低的熒光背景。Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent
方法根據抗原抗體特異性結合的原理,首先對抗a 、抗b單克隆抗體進行熒光標記,然後使熒光標記抗體與相應抗原(血痕)在最佳條件下結合,最後熒光顯微鏡鏡檢,判定血痕的血型。The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結合以上創建的方法和免疫熒光流式細胞術,用k562細胞株可溶性分泌物(上清)對外周血單個核細胞( pbmc )進行培養以模擬體內微環境,然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究,結果發現用腫瘤上清培養的pbmc細胞數量下降明顯,但同時對其有激活作用,且呈劑量依賴性;細胞數的下降主要是由細胞壞死和凋亡引起的,腫瘤上清對細胞周期沒有阻斷作用,反而略有促進作用; t細胞亞群比例增加,並促進表達th1 、 th2細胞因子。In this experiment three sperm proeins, sp - 10. aeg. tmdc - iii. that were cloned and expressed by brist u. k., immunized on rats for analysing their immunogenicity and the effects on the fertility. then using indirect immuno - fluorescein staining method, the localization of sp - 10, tmdc - iii and aeg on human, mouse, rabbit, pig, bull sperm was observed under confocomicroscopy. the results as follows : 1. the rats successfully produced antibody after immunization with antigens sp - 10, aeg, tmdc - iii respective
本文用英國葛蘭素公司重組構建表達的三種抗原蛋白sp - io , aeg , tmdc -免疫大鼠,通過elisa實驗、免疫熒光組化實驗、交配實驗對它們的免疫原性、在體內的生殖作用、精子表達部位進行了分析,結果如下: 1Antibodieswhich are tagged bind to antigens and emit fluorescent signal ( shown asthe yellow star ) which can then be detected from the spot on the array
抗體抗原結合所放出的熒光標記信號(顯示為黃色的星) ,然後再從現場發現的陣列In the second trial, this modified discontinuous percoll gradient centrifugation method was introduced to isolate spermatids from the semen of fifteen male infertile patients. then the effect was identified by wright - giemsa stain, flow cytometry analysis, immunocytochemistry and fluorescence in situ hybridization ( fish ). similary, the 22 % percoll fraction contained mostly haploid cells [ ( 91. 85 ? 5. 18 ) % ] ( p < 0. 005 ) and the mean density in this fraction was ( 1. 010 ? 0. 786 ) x 105 / ml
C法,對15例各種類型不育患者的精液細胞進行分離,並利用瑞姬染色法、流式細胞術、免疫細胞化學和熒光原位雜交oisffi等方法,從細胞形態特徵、 dna倍體、細胞表面標i己與分化抗原,以及原位雜交信號的數目和位置結合細胞核特有的形態等方面加以鑒定。分享友人