熒光標記引物 的英文怎麼說
中文拼音 [yíngguāngbiāojìyǐnwù]
熒光標記引物
英文
fluorescently labeled primer- 熒 : 形容詞[書面語]1. (光亮微弱的樣子) glimmering 2. (眼光迷亂; 疑惑) dazzled; perplexed
- 光 : Ⅰ名詞1 (照耀在物體上、使人能看見物體的一種物質) light; ray 2 (景物) scenery 3 (光彩; 榮譽) ...
- 標 : Ⅰ名詞1 [書面語] (樹梢) treetop; the tip of a tree2 (枝節或表面) symptom; outside appearance; ...
- 記 : Ⅰ動詞1 (把印象保持在腦子里) remember; bear in mind; commit to memory 2 (記錄; 記載;登記) writ...
- 引 : Ⅰ動詞1 (牽引; 拉) draw; stretch 2 (引導) lead; guide 3 (離開) leave 4 (伸著) stretch 5 (...
- 物 : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
- 標記 : (標志; 記號; 貨物標記) tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
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The purposes of our work are to establish a simplified method of multiplex pcr based on chimeric primers for str loci, to develop a set of fluorescent quadriplex str system for forensic dna typing based on this method, and to validate the forensic application of the system under the guidelines of tmgdam ( the technology working group on dna analysis methods ) in order to address concerns presented in today ' s legal environment
目的本課題旨在探索一種新的str基因座復合擴增方法,我們稱為嵌合引物str復合擴增法。應用熒光標記毛細管電泳激光自動檢測技術平臺,建立一套新的法醫str基因座復合擴增體系,並按照美國dna分析方法技術工作組( thetechnologyworkinggroupondnaanalysismethods , twgdam )的指導方案進行法醫學實用性研究。7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp
7 、首次以fam熒光素標記探針5 』端作為發光基團,以tarma標記探針3 』端為淬滅基團,以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a )為檢狽目標,設計、篩選出特異性引物和探針,優化實驗參數,建立了轉基因植物通用性熒光pcr定性檢測方法體系。Pcr amplification of the target sequence design a pair of primers ac - coding to the conservative region of hla - dqa1 exon 2, and lable the sence strand by fitc. make the fiuorescenced strand the advantage strand by assym - metric pcr. 3
樣本靶序列的pcr擴增在hla - dqa1第二外顯子保守區域設計一對引物,並在正義鏈引物5 』作fitc標記,以熒光標記單鏈為優勢鏈,行不對稱擴增。The amplification system was optimized so that the pcr with different primers can be carried out under the same condition. the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques. sequences were analyzed and compared base on sequencing analysis3. 4 and seq / ede software
方法根據mtdna控制區及其周圍區域的序列,設計多對引物,探索優化擴增體系,使擴增條件能夠同時滿足多對引物的需要,用sanger末端終止法及熒光標記技術對樣本進行dna測序, sequencinganalysis3 . 4和seq ede軟體進行序列分析和比對。Result. a method of multiplex pcr based on both chimeric and universal primers was established. a fluorescent quadriplex str system, including d1s1612, d9s2026, d20s161 and d6s477, was developed on the basis of the multiplex pcr
建立了dis1612 、 d952026 、 d205161 、 d65477str基因座熒光標一記嵌合引物復合擴增毛細管電泳激光自動分析體系,同時證明嵌合引物復合擴增方法與毛細管電泳激光自動分析方法相兼容。The use of chimeric primers provides a method for primer design that eliminates the multiple optimization steps involved in developing multiplex pcr. our fluorescent quadriplex str system can be analyzed by capillary electrophoresis and provide a new sources of typing regent for this technique platform with laser detecting system
結論建立了法醫四個遺傳標記dis1612 、 d952026 、 d205161 、 d65477新的熒光標記嵌合引物復合擴增體系,證明該體系可成功應用於熒光標記毛細管電泳激光自動分析檢測平臺。分享友人