熒光標記引物 的英文怎麼說

中文拼音 [yíngguāngbiāoyǐn]
熒光標記引物 英文
fluorescently labeled primer
  • : 形容詞[書面語]1. (光亮微弱的樣子) glimmering 2. (眼光迷亂; 疑惑) dazzled; perplexed
  • : Ⅰ名詞1 (照耀在物體上、使人能看見物體的一種物質) light; ray 2 (景物) scenery 3 (光彩; 榮譽) ...
  • : Ⅰ名詞1 [書面語] (樹梢) treetop; the tip of a tree2 (枝節或表面) symptom; outside appearance; ...
  • : Ⅰ動詞1 (把印象保持在腦子里) remember; bear in mind; commit to memory 2 (記錄; 記載;登記) writ...
  • : Ⅰ動詞1 (牽引; 拉) draw; stretch 2 (引導) lead; guide 3 (離開) leave 4 (伸著) stretch 5 (...
  • : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
  • 標記 : (標志; 記號; 貨物標記) tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
  1. The purposes of our work are to establish a simplified method of multiplex pcr based on chimeric primers for str loci, to develop a set of fluorescent quadriplex str system for forensic dna typing based on this method, and to validate the forensic application of the system under the guidelines of tmgdam ( the technology working group on dna analysis methods ) in order to address concerns presented in today ' s legal environment

    目的本課題旨在探索一種新的str基因座復合擴增方法,我們稱為嵌合str復合擴增法。應用毛細管電泳激自動檢測技術平臺,建立一套新的法醫str基因座復合擴增體系,並按照美國dna分析方法技術工作組( thetechnologyworkinggroupondnaanalysismethods , twgdam )的指導方案進行法醫學實用性研究。
  2. 7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp

    7 、首次以fam探針5 』端作為發基團,以tarma探針3 』端為淬滅基團,以camv35s 、 fmv啟動子、 nos終止子、基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a )為檢狽目,設計、篩選出特異性和探針,優化實驗參數,建立了轉基因植通用性pcr定性檢測方法體系。
  3. Pcr amplification of the target sequence design a pair of primers ac - coding to the conservative region of hla - dqa1 exon 2, and lable the sence strand by fitc. make the fiuorescenced strand the advantage strand by assym - metric pcr. 3

    樣本靶序列的pcr擴增在hla - dqa1第二外顯子保守區域設計一對,並在正義鏈5 』作fitc,以單鏈為優勢鏈,行不對稱擴增。
  4. The amplification system was optimized so that the pcr with different primers can be carried out under the same condition. the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques. sequences were analyzed and compared base on sequencing analysis3. 4 and seq / ede software

    方法根據mtdna控制區及其周圍區域的序列,設計多對,探索優化擴增體系,使擴增條件能夠同時滿足多對的需要,用sanger末端終止法及技術對樣本進行dna測序, sequencinganalysis3 . 4和seq ede軟體進行序列分析和比對。
  5. Result. a method of multiplex pcr based on both chimeric and universal primers was established. a fluorescent quadriplex str system, including d1s1612, d9s2026, d20s161 and d6s477, was developed on the basis of the multiplex pcr

    建立了dis1612 、 d952026 、 d205161 、 d65477str基因座嵌合復合擴增毛細管電泳激自動分析體系,同時證明嵌合復合擴增方法與毛細管電泳激自動分析方法相兼容。
  6. The use of chimeric primers provides a method for primer design that eliminates the multiple optimization steps involved in developing multiplex pcr. our fluorescent quadriplex str system can be analyzed by capillary electrophoresis and provide a new sources of typing regent for this technique platform with laser detecting system

    結論建立了法醫四個遺傳dis1612 、 d952026 、 d205161 、 d65477新的嵌合復合擴增體系,證明該體系可成功應用於毛細管電泳激自動分析檢測平臺。
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