熒光標記抗體 的英文怎麼說
中文拼音 [yíngguāngbiāojìkàngtǐ]
熒光標記抗體
英文
fluorescent-labeled antibody- 熒 : 形容詞[書面語]1. (光亮微弱的樣子) glimmering 2. (眼光迷亂; 疑惑) dazzled; perplexed
- 光 : Ⅰ名詞1 (照耀在物體上、使人能看見物體的一種物質) light; ray 2 (景物) scenery 3 (光彩; 榮譽) ...
- 標 : Ⅰ名詞1 [書面語] (樹梢) treetop; the tip of a tree2 (枝節或表面) symptom; outside appearance; ...
- 記 : Ⅰ動詞1 (把印象保持在腦子里) remember; bear in mind; commit to memory 2 (記錄; 記載;登記) writ...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 體 : 體構詞成分。
- 標記 : (標志; 記號; 貨物標記) tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
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The data above showed that algae phycobiliprotein fluorescent probes not only facilitate the study to pea actins but also provide a doable method to label other plant proteins. it has a good foreground for algae fluorescence probe to be used in the immunity fluorescent detection of plant cells. the gene clon
對豌豆肌動蛋白藻熒光探針的初步研究,不僅為豌豆肌動蛋白的免疫熒光檢測提供了便利條件,而且通過改變抗體,則可以將藻熒光探針用於其他植物蛋白的標記,藻熒光探針優越的熒光特性使其在植物材料的免疫熒光檢測中具有廣闊的應用前景。7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp
7 、首次以fam熒光素標記探針5 』端作為發光基團,以tarma標記探針3 』端為淬滅基團,以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a )為檢狽目標,設計、篩選出特異性引物和探針,優化實驗參數,建立了轉基因植物通用性熒光pcr定性檢測方法體系。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選,在pap基因的3 』端融入了綠色熒光蛋白gfp )基因,然後將融合基因克隆在植物表達載體pbi121上,再進行遺傳轉化,得轉基因棉花種子5000餘粒,將種子播種長到于葉展開時,先在黑暗中用紫外燈照射,查找表現綠色熒光的幼苗,然後再用地高辛( dig )標記的pap基因特異性探針對這些棉花進行點雜交,最後發現有8株棉花表現陽性反應,說明pap基因的確己經轉到了棉花的基因組中,其棉花黃萎病的抗性鑒定正在進行之中。In this paper, wheat cultivars lovrin 10 ( resistant ) and 5389 ( susceptible ) were selected as materials in this system. the mesophyll protoplasts of wheats ( mpw ) were isolated using cellulase and pectolase digestion. by the indirect immune fluorescent labeling, microtubules ( mts ) pattern in mpw were showed clearly under the confocal laser scanning microscope ( clsm )
本試驗以抗(洛夫林10 ) 、感( 5389 )不同的兩小麥( triticumaestivuml . )品種為材料,採用酶解法制備小麥葉肉原生質體,利用間接免疫熒光方法結合激光共聚焦掃描顯微技術對小麥葉肉細胞原生質體的微管骨架進行了清晰的標記,並探索了微管骨架標記的影響因素。. from the direct mutant of spirulina platensis ( sp - d ), we got high purity and activity phycobiliprotein which could grow crystals. the algae fluorescent probe prepared by coupling the above polyclonal antibody to phycobilipotein not only keeps the property of stronger anti - fluorescence quenching but also has the lower fluorescent background when it was used for labeling stoma cells of pea tendril
以原核表達的peac1為抗原制備了免疫活性較好的抗豌豆肌動蛋白的多克隆抗體,從螺旋藻中純化了高純度、高活性、能結晶的藻膽蛋白,將兩者偶聯制備的藻熒光探針,不僅保持了藻膽蛋白很強的抗熒光淬滅能力,而且用於豌豆卷須氣孔細胞熒光標記時有更低的熒光背景。Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent
方法根據抗原抗體特異性結合的原理,首先對抗a 、抗b單克隆抗體進行熒光標記,然後使熒光標記抗體與相應抗原(血痕)在最佳條件下結合,最後熒光顯微鏡鏡檢,判定血痕的血型。On the other hand, our previous studies have shown that the nr2b construct with a gfp tag in its n - terminus cotransfected with nr1 subunit can reach the cell surface and be detected by surface staining with anti - gfp antibody in live cells. the nr2b construct will be retained in the endoplasmic reticulum ( er ) when expressed alone in heterologous cells
我們以往的研究表明,用綠熒光蛋白( gf內在信號膚下游n末端標記nrzb亞單位,與nri共轉染時能形成與野生型相似的nmda受體通道,且可以通過抗gfp抗體標記活細胞膜表面表達的nmda受體復合物。Objective to explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material
摘要目的探討採用熒光免疫標記抗體法測定血痕血型的可行性和優點。Antibodieswhich are tagged bind to antigens and emit fluorescent signal ( shown asthe yellow star ) which can then be detected from the spot on the array
抗體抗原結合所放出的熒光標記信號(顯示為黃色的星) ,然後再從現場發現的陣列分享友人