熒光顯微 的英文怎麼說
中文拼音 [yíngguāngxiǎnwéi]
熒光顯微
英文
fluorescence microscopy-
Equipment and instruments : electronic analytic balance, uv and vis spectrophotometer, 97rt fluorescence spectrophotometer, gas chromatograph spectrometer, high speed centrifugal machines, leica rm 2015 microtome, fluorescence microscopes, pcr amplifier, and so on
:電子分析天平、紫外可見光分光光度計、 97rt熒光分光光度計、氣相色譜儀（附4種檢測器） 、高速離心機、病理切片機、熒光顯微鏡、 pcr擴增儀等。Mycobacteria can also be stained with auramine and viewed with fluorescence microscopy, in which acid fast bacilli now appear as glowing yellow rods
分枝桿菌也能被金胺染色，熒光顯微鏡下抗酸桿菌為發黃*色熒光的桿菌。Laser confocal fluorescence microscopy
激光共聚焦熒光顯微鏡On the backgrounds of researches inside and outside country, and cooperating experiments with theories analyses, the influence of different processing technology parameters and different sbs modifier sorts on the sbs modified asphalts " properties has been studied. at the same time, their microstructure are observed through fluorescence optical microscopy and scanning electronic microscopy, thus to direct modified asphalt production. on the above conclusion ' s basement, analysing some disadvantages of the storage stability test of sbs modified asphalt in the current specification, a new storage stability test apparatus is developed
本文在參考國內外研究的基礎上，採用理論、試驗相結合的方法，研究加工工藝參數以及改性劑種類等對sbs改性瀝青性能的影響，並通過熒光顯微鏡、掃描電鏡分析其微觀形態，從而指導sbs改性瀝青的生產；在此基礎上，分析我國現行規范用來評價sbs改性瀝青儲存穩定性方面的不足，開發了新的試驗儀，根據動態剪切流變試驗結果和微觀狀態分析，提出一個新的指標? ?離析率r _ s來評價sbs改性瀝青的儲存穩定性；最後，針對不穩定的改性瀝青提出改善措施，研究證明摻加增容劑和穩定劑是行之有效的方法。We investigated the distribution of the heterotrophic bacteria with the epifluorescence microscope and measured the bacterial production with the tritiated tymicline incorporation method, and we investigated the correlation between the heterotrophic bacteria and chlorophyll, inorganic nitrogen also. there was distinct spatial distribution of the bacterial biomass in the east china sea and the yellow sea during fall and spring
本文利用表面熒光顯微鏡觀測計數法和[甲基- 3h ]胸腺嘧啶示蹤法對春秋兩季節我國黃、東海異養細菌生態分佈及其生產力狀況，以及異養細菌及其生產力與浮游植物葉綠素、無機氮鹽之間的關系進行了研究。By compressing a monolayer film, the coexistence of liquid condensed ( lc ) and liquid expanded ( le ) phases can be reached. the transition from le to lc is usually regarded as a first - order one, so the theory of crystallization can be applied. in this article we review our recent studies on the growth of lc domains in the le - lc coexistence region driven by the illumination of a fluorescent microscope. the mechanism of this unusual 2d domain growth phenomenon is discussed. the formation of faceted, dendritic and fractal - like domains as well as the evolution and the transition of these patterns are investigated
當處于氣液界面的類脂類化合物的單分子膜被壓縮時，隨著分子間距的縮小，單分子膜將經歷一系列相變過程.通過熒光顯微術可以觀測到新相的成核和生長過程.由於單分子膜的二維特性，該系統中的實驗觀測對于檢驗和發展二維界面生長理論尤為重要.本文總結了近年來本課題組與相關單位合作，在單分子膜系統中發現的實驗現象以及對其生長機制的系列研究.內容包括對單分子膜系統中的成核、界面穩定性、枝晶生長、形態演變等的觀測和分析Abstract : by compressing a monolayer film, the coexistence of liquid condensed ( lc ) and liquid expanded ( le ) phases can be reached. the transition from le to lc is usually regarded as a first - order one, so the theory of crystallization can be applied. in this article we review our recent studies on the growth of lc domains in the le - lc coexistence region driven by the illumination of a fluorescent microscope. the mechanism of this unusual 2d domain growth phenomenon is discussed. the formation of faceted, dendritic and fractal - like domains as well as the evolution and the transition of these patterns are investigated
文摘:當處于氣液界面的類脂類化合物的單分子膜被壓縮時，隨著分子間距的縮小，單分子膜將經歷一系列相變過程.通過熒光顯微術可以觀測到新相的成核和生長過程.由於單分子膜的二維特性，該系統中的實驗觀測對于檢驗和發展二維界面生長理論尤為重要.本文總結了近年來本課題組與相關單位合作，在單分子膜系統中發現的實驗現象以及對其生長機制的系列研究.內容包括對單分子膜系統中的成核、界面穩定性、枝晶生長、形態演變等的觀測和分析The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter, length and shape being identical to that of muscle f - actins, which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ), and were identified as having a 9 nm diameter by negative staining, corresponding with that of the muscle f - actins ( 7 - 10 nm ). under polymerization conditions, his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin, that is, an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained. the critical concentration is 0. 75 umol / l near to that of chicken muscle actin ( 0. 56 umol / l ) under the same condition
熒光標記結合熒光顯微觀察表明：從可溶性上清中純化的his - taggedpeac1 - gfp聚合形成的微絲不僅可以直接在熒光顯微鏡下觀察，也可被微絲的特異標記物鬼筆環肽所標記，而且其直徑、長度以及形態上與已知的聚合肌動蛋白熒光絲一致；電鏡負染的結果進一步證實其直徑為9nm ，與傳統微絲直徑相當（ 7 ? 10nm ） ；聚合曲線有明顯的停滯期，為典型的s型聚合曲線，聚合臨界濃度為0 . 75 mol l ，這一結果與已有報道相似。After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells, the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope, indirect immunofluorescence and flow cytometer analysis. prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed
建系細胞培養上清與肝癌細胞作用后，經熒光顯微鏡觀察、間接免疫熒光及流式細胞儀檢測進一步確定表達的scfv融合蛋白具有與hbsag特異性結合的活性。We fused the gfp into the c - terminal region as well as n - terminal region of emt - 1 protein. the result showed that the localization of the protein encoded by the full length emt - 1 cdna was in endoplastic reticulum ( er ). extracellular region, transmembrane and intracellular region showed the similar cellular localization
我們用綠色熒光蛋白融合於emt l全長及其不同截斷形式的梭基和氨基端，瞬時轉染cos 7細胞，通過熒光顯微鏡觀察，發現emt l編碼的蛋白呈現內質網定位的特點。We studied development mechanism by the distribution of microfilaments and actin mrna in cotton callus, healtny plants and abnormal plantlets. fitc - phalloidin as fluorescence probe was used to investigate the meristem of the cotton root, abnormal plantlets and callus that was unable to germinate into healthy plants
本研究選取正常棉花的根，已經培養了長時間不能分化出正常植株的棉花愈傷組織和棉花畸形苗為材料，採用石蠟切片，通過fitc -鬼筆環肽對材料微絲熒光染色，結合熒光顯微鏡觀察。We made a living cell observation on the effect of w7 treatment to the ingression of the cleavage furrow
我們在倒置熒光顯微鏡下跟蹤觀察了w7處理對細胞分裂溝縊裂加深的影響。Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent
方法根據抗原抗體特異性結合的原理，首先對抗a 、抗b單克隆抗體進行熒光標記，然後使熒光標記抗體與相應抗原（血痕）在最佳條件下結合，最後熒光顯微鏡鏡檢，判定血痕的血型。Twophoton laser scanning fluorescence microscopy and its applications
雙光子激光掃描熒光顯微鏡及其應用Fluorescent microscope observation results indicated there was fluorescence in ybt1765 - 72b. but there was no fluorescence in y
熒光顯微鏡觀察結果表明ybt1765一72b在激發光下可以發射綠色熒光。The approaches used in the study included : observing the microstructure and ultrastructure of the cell line of colossoma brachypomum ( cbt ) and the cell line of carp ( cp ) stressed low temperatures under fluoroscopy and tem ; analysis of dna damage in the cultured cells under temperatures stress by dna gel electrophoresis
本研究採用的主要實驗方法：通過熒光顯微觀察、電鏡超微結構觀察確定cbt （淡水白鯧臀鰭細胞）和cp （草魚胚胎細胞）在低溫處理后的顯微與超微結構的變化。應用dna電泳分析細胞dna在低溫處理后的斷裂現象。A mixture of three amino acids ( arg, gly, glu ) labeled with fluorescein isothiocyanate ( fitc ) was separated in pdms microfluidic chip, the separation voltage is 200v / cm, the separation time is less than 120 seconds ; according to ccd fluorescence images, two distinct physical processes - stacking and destacking during sample injection were studied qualitatively ; rhodamine b, a kind of temperature - dependent fluorescence dye, was used as probe to develop a temperature - fluorescence intensity equation, then temperature - color map in microchannels was constructed, and temperature trait in microchannels on the pdms microfluidic chip was analysed. according to the results, we conclude that the electric field applied to the pdms microfluidic chip should not exceed 400v / cm
利用pdms微流控晶元對fitc標記的精氨酸、甘氨酸、谷氨酸混合物進行了電泳分離，分離電壓為200v cm ，分離時間不到120秒；通過拍到的熒光顯微圖像對電泳注樣過程中復雜的樣品分子積聚與解聚現象作定性的分析；以熒光染料rhodamineb為溫度熒光探針，建立了pdms微流控晶元上的溫度-熒光強度的關系公式，並利用matlab圖像處理工具箱構建出微流體溝道內的溫度色圖，對pdms微流控晶元的微流道溫度特性進行了分析，根據實驗結果，我們認為對于pdms微流控晶元來說，在進行需要外加電場作用的試驗時，外加電場不應超過400v cm 。Test method for enumeration of aquatic bacteria by epifluorescence microscopy counting procedure
按熒光顯微鏡計算程序進行水生菌計數的試驗方法According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr
然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。Test method rapid enumeration of bacteria in electronics - grade purified water systems by direct - count epifluorescence microscopy
用直接計數淺熒光顯微鏡進行電子級凈化水系統中細菌的快速記數的試驗方法分享友人