片段化蛋白 的英文怎麼說

中文拼音 [piānduànhuàdànbái]
片段化蛋白 英文
fragmin
  • 片 : 片構詞成分。
  • 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. We focused on the following aspects ; 1 ) we first assayed the expression of complement receptors and complement - associated molecules on distinct subsets of dendritic cells during their development in order to understand the physical basis of the sensitivity of dendritic cells to complement and its split products ; we next studied the effects of complement activation on the survival of dendritic cells during their development ; and finally examined the effects of the whole complement system, focusing on the ability of one of the split products of complement activation, c5a, and its first subcomponent - c1q, to influence chemotaxis of dendritic cells, as well as allo - t cell stimulatory activity of dc

    我們通過免疫磁珠分離了兩種人dc前體，即髓系來源的單核細胞（ monocytes ， mo ）和淋巴系來源的漿細胞樣dc （ plasmaeytoiddendriticeells ， pdc ） ，對這兩個不同dc亞群進行體外誘導培養，使其處于不同的分化發育階段，然後檢測了其表達補體受體一cd35 （ cri ） 、 cd21 （ crz ） 、 cdilb （ cr3 ） 、 cdlle （ cr4 ） ，補體調控蛋白一cd46 、 cd55 、以及部分補體片斷分子受體一c3ar 、 csar 、 clqrp的水平。
  3. Confocal microscopy observation followed immune - fluorescence staining with anti - gp130 showed that gp130 could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus

    為了進一步證實上述發現，我們表達並純化了gst - gp130胞漿區融合蛋白和gst - tle1獨特性片段融合蛋白，並制備了特異性抗tle1多克隆抗體。
  4. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. The localization and expression of prolactin receptor from inner mongolia alpas cashmere goat were studied by sacpic staining, in situ hybridization and western blotting. samples of skin were taken at interval three months from birth, three months old, six months old, nine months old, ten months old or twelve months old, which correspond to summer, autumn, winter and spring. paraffin sections of hair follicles were stained with sacpic staining and in situ hybridization. the protein of prolatin receptor is abstracted from samples of skin in order to study on expression of prolactin receptor. there are prolactin receptors in outer root sheath, dermal papilla and inner root sheath. the growth of primary follicle is continuous

    本實驗從絨山羊出生后每隔三個月采一次皮樣，共分為4個月齡（ 3 、 6 、 9 、 10或12 ）段，通過製作石蠟切片，原位雜交、染色，並提取皮樣蛋白做westernblotting等實驗研究方法，研究了催乳素受體mrna催乳素受體在不同生長季節的內蒙古阿爾巴斯白絨山羊皮膚毛囊中的定位與表達，染色結果發現阿爾巴斯白絨山羊初級毛囊全年持續生長，次級毛囊的生長情況隨季節而變化，秋冬季生長旺盛，夏季生長緩慢與絨毛生成規律呈正相關。
  7. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  8. In order to check if it is the aim gene, we devised pcr with a new pair of primer, sequenced and registered the product with registration number : af449446. moreover we forecast and analysis the primary, secondary, tertiary and quaternary structure of the three protein : osftszi, crftszi and crftsz2 which has already cloned by our team before. after that we construct ftsz molecular evolution tree to site them in

    又將生物信息學技術同實驗技術相結合，針對ftsz保守區設計引物擴增出一條衣藻ftsz片段，進行est搜索、比對、拼接，最終克隆出新基因crftsz1 ；連同本試驗室曾經獲得的另一個衣藻crftsz2基因進行蛋白質的一、二、三、四級結構預測、分析及比較尋找進化線索，建立了ftsz蛋白的氨基酸進化樹作進一步的進化定位。
  9. This research use high sensitive, special in situ pcr technology to determine the material which is treated by the paraffin wax and formalin fixation of different time, by detecting the genotype of mn blood group of the organizes slices. at the same time, we study the research of main influence factors, such as protease digestion, etc. we hope to set up a kind of steady, practical methods to detect the genotype of the material treated by paraffin wax and formlin, offer a kind of new detecting means for the forensic appraises and iditificition with individual material evidence

    本研究應用靈敏度高、特異性好的原位pcr技術，測定福爾馬林固定不同時間的石蠟切片組織mn血型的基因型，並對蛋白酶消化時間等主要影響因素進行了初步的研究，以建立一種穩定、實用的檢測石蠟組織切片dna遺傳標記的方法，為法醫物證鑒定和個人識別提供一種新的檢測手段。
  10. There is no characteristic in the amino acid sequence 63 - 152 and it is the piece that we want to delete to identify the function of the segment. ie180 gene mutants deleted the 64 - 151 amino acid was amplified by muti - pcr and were cloned by pmdist - vector. the clone plasmids were named pjmp1p3p2. the segment corresponding to the sequence of 1 - 1079 amino acid of the genbank sequence amplified by pcr, its clone plasmids was named pjmp1p2. the segment corresponding to the sequence of 454 - 1079 of genbank sequence amplified by pcr and its clone plasmids is named pjmp6p2 - three clone plasmids and pcdna3 were digested by restriction enzyme bamhi and hind, the gene segment of p1p2, p1p3p2, p6p2 were recycled

    本試驗應用dnamis及prosis軟體分別對genbank中登記號為no352564的ie180序列進行了蛋白質序列分析，結果表明其1 - 34段氨基酸序列具有典型helix - turn - helix特徵序列，並且富含酸性氨基酸d及e ，是典型的dna識別序列；富含絲氨酸序列的152 - 409氨基酸序列是一個與激活有關的、潛在的磷酸化位點； 454 - 696氨基酸區域是dna結合域； 64 - 151氨基酸片段沒有明顯的序列特徵；從中可看出ie180蛋白的1 - 1080氨基酸段具有典型的轉錄激活因子結構特徵。
  11. After pcr checking and electro - transformation plasmid from c. elegans into dh5a, isolating plasmid from dhsct, digesting them with restriction enzymes and dna sequencing, six cdna fragments, which protein products can interact with rapgap, from cdna library were got

    將pcr擴增陽性及或lacz強陽性質粒電轉化入dhsa細菌，提取質粒后酶切、測序。發現有6個來源於celegqnscdna文庫的dna片段編碼的蛋白可以與ra帥ap相互作用，其中兩個為rapgap 。
  12. It " s the first cdna code ( 6 - 4 ) photolyase found in low orgnism ( alga ), and this study is important for the reseach of 6 - 4 photolyase. the methods and analysis as bellows : 1. this est was analyzed by method of blast, and the conclusion suggests that the sequence probably be a partial cdna that can code one of protein family of photolyase / blue light photoreceptor

    利用3 』 race法擴增此cdna序列的3 』端部分，所得片段長度為2575bp ，含一個完整的開放讀框，長1800bp ，可編碼600個氨基酸；利用生物信息學法對此擴增序列進行分析，包括同源性分析、序列的讀框分析、蛋白質的保守性分析以及該蛋白的進化關系分析，預測出此序列編碼了d . salina的（ 6 - 4 ）光裂合酶。
  13. But it is doubt that the speciality and stability for the outcome of detecting bdv orf i genome. we will detect the indviduals ' ( include the neuropsychiatric 4 patients with bdv orfii postive or not and healthy blood donator ) middle fragment of bdv orf i to help defmiting whether internal healthy people and the certain neuropsychiatric patient such as with viral encephalitis, multiple, schizophrenia, affective disorder have catch a bdv infection or not, most of all, the research is for understanding if there is any difference in the fragment from animal and human beings infected with bdv, thereby to find another sensitive and stably marker for confirming bdv infection, and establish a solide bottom for advanced study

    但對另一編碼p40蛋白序列的檢測結果的特異性和穩定性還不肯定，我們通過對先前檢測bdvorf基因片段為陽性或陰性的神經精神病人和健康獻血者進行了bdvorf基因中部片段的檢測。以幫助確定在國內健康人群和某些神經精神疾病患者中如：病毒性腦炎、多發性硬化、精神分裂癥以及情感障礙等疾病中是否存在bdv的感染，更為重要的是了解感染人和動物的該病毒片段是否存在差異，從而找出另一檢測bdv感染的敏感性和穩定性的標記物，為下一步的研究奠定良好的基礎。
  14. A 1. 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites. the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge. strain dh5a of e. coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so

    以質粒ppgedna為模板， pcr擴增出1 . 7kb的ge基因完整片段，將擴增產物以ecori和bamhi雙酶切后，插入原核表達載體pbv220的p _ rp _ l啟動子下游的ecori和bamhi位點間，得到重組表達質粒pbvge ，轉化了pbvge的大腸桿菌dh5a經溫敏誘導表達后，用sds - page和western - blot ，以及瓊脂雙擴散來檢測，結果表明prvfa株ge基因在原核載體上得到高效表達，表達產物約占總蛋白的17 。
  15. These results suggested that axud1 protein functioned in a different way in the expression of cell cycle and apoptosis related proteins induced by tgf - 1 in tumor cells. 5. axudl cdna fragment was cloned into procaryotic expression vector pqe30 and t

    5 . axudlcdna片段成功地克隆入原核表達載體pqe30 ，並用iptg誘導出axudi融合蛋白的表達，這為進一步純化axudi蛋白，研究axudi蛋白的免疫組織化學定位和結構功能奠定了基礎。
  16. We constructed the pgex - gdnf expression vecto ' rs and obtained the recombinant protein of expected size after induction of iptg. the fusion protein was purified using glutathione sepharose 4b affinity chromatography

    將大熊貓和朱?的gdnf基因片段分別克隆至pgex ? 4t ? 3表達載體，並經iptg誘導，表達出了預期大小的蛋白，該蛋白用glutathionesepharose4bmicrospincolumn進行了純化。
  17. For these goals, the fo llowing jobs have been done and some results have been obtained. 1 according to the vitreoscilla hemoglobin ( vhb ) amino acid sequence and plajnt preference codon usage, vgbm gene was designed and synthesized by annealing 22 synthetic fragments respectively, the modified vgbm gene of full length of 450 base pairs was synthesized. 2 the transformants were obtained after pbv221svhb was introduced into e. coli

    為此本論文作了以下工作並得出了一些結論： 1根據透明顫菌血紅蛋白（ vhb ）的氨基酸序列，選用植物偏愛密碼子，對透明顫菌血紅蛋白基因（ vgb ）進行優化改造，設計併合成了22條寡核苷酸短片段，人工合成了改造的全長450bp的透明顫菌血紅蛋白（ vhb ）基因（ vgbm
  18. The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach

    為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
  19. Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme

    作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。
  20. In immuno - blotting, these fragments reacted specifically with hepatitis c patients " sera, suggesting that e. coli - derived e2 proteins carried hcv e2 - specific, glycosylation - and - conformation - independent epitopes

    在免疫印跡檢測中，上述片段與丙型肝炎病人血清有特異性反應，表明大腸桿菌系統表達的e2蛋白攜帶有hcve2特異的、不依賴于糖化和立體構象的抗原決定簇。
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