琥珀酰酶 的英文怎麼說
中文拼音 [hǔpò]
琥珀酰酶
英文
succinyl enzyme-
E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd
以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ,以輔助噬菌體m13k07超感染,進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein. the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis. it was demonstrated that constructed phagemid can still express penicillin g acylase
將包含信號肽和琥珀終止密碼子uag ( amber )的完整巨大芽孢桿菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ,通過引入的11肽連接青霉素g酰化酶的c末端與噬菌體外殼蛋白gp3的n末端。
分享友人