生電母細胞 的英文怎麼說
中文拼音 [shēngdiànmǔxìbāo]
生電母細胞
英文
electroblast- 生 : Ⅰ動詞1 (生育; 生殖) give birth to; bear 2 (出生) be born 3 (生長) grow 4 (生存; 活) live;...
- 電 : Ⅰ名詞1 (有電荷存在和電荷變化的現象) electricity 2 (電報) telegram; cable Ⅱ動詞1 (觸電) give...
- 母 : Ⅰ名詞1 (母親) mother 2 (泛指女性長輩) one s elderly female relatives 3 (配套的兩件東西里的凹...
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Results the major morphologic changes were as follows : histologically, alveolar inflammation and interstitial fibrosis were observed. electron microscopic findings were : 1. alveolar type i cells were degenerated 、 broken - down and desquamated, endothelial cells were swelled, with inter cellular tight junction shortened, alveolar type ii cells hyperplastic, basement membrane thinned and deformed ; 2. alveolar macrophages and interstitial macrophages were hyperplastic ; 3. mast cells were infiltrated and degranulated ; 4. electron - dense deposits were present at alveolar wall ; 5. myofibroblasts 、 fibroblasts 、 collagen and basement membrane like material were hyperplastic
電鏡觀察可見: ( 1 ) i型肺泡上皮細胞變性、崩解和脫落,內皮細胞腫脹,細胞間緊密連接短小, ii型肺泡上皮細胞增生,基底膜變薄和破壞; ( 2 )肺泡巨噬細胞、間質巨噬細胞增多; ( 3 )肥大細胞浸潤並見脫顆粒現象; ( 4 )肺泡壁電子緻密物沉積; ( 5 )肌纖維母細胞、纖維母細胞、膠原原纖維及基底膜樣物質增生。Studies on spermatogenesis and oogenesis in palaemon modestus jiang ye - qin ( chemistry department, huzhou teachers coliege, huzhou 313000 ) palaemon modestus belongs to genus palaemon, family palaemonidae, caridea, natantia, decapoda, class crustacean. lt is a kind of freshwater prawn across china and especially abounds in the taihu lake which is regarded as one of the " three delicacies " of the taiha lake. as for the researches on palaemon modestus, v / e can only refer to spermatogenesis of freshwater shrimp exopalaemon modestus ^ ruang hai - xia et al, 2001 ), studies on reproductive biology of exopalaemon modestus l. the structure and development of the male reproductive system ( huang hai - xia et al, 1999 ) and studies on freshwater prawn in the taihu lake ( yan sheng - liang, 1999 ). on the bases of their researches and with the help of tem, i have made further researches on sperm ultrastructure, spermatogenesis, oogenesis and mature oocyte ultrastructure in palaemon modestus
秀麗白蝦palaemonmodestus屬甲殼綱crustacea十足目decapoda游泳亞目natantia真蝦部caridae長臂蝦科palaemonidae長臂蝦屬palaemon ,是我國南北均產的淡水蝦,其中太湖產量尤其大,與太湖銀魚、鱭魚並稱「太湖三寶」 。有關秀麗白蝦的研究僅見秀麗白蝦雄性生殖系統的研究(黃海霞等, 1999 ) 、秀麗白蝦精子發生的研究(黃海霞等, 2001 ) 。本人在前人工作的基礎上,利用透射電鏡技術( tem )進一步研究了秀麗白蝦精子的形態、結構及精子的發生過程,同時還研究了秀麗白蝦卵的發育過程,從卵原細胞到卵黃發生前的卵母細胞、卵黃發生的卵母細胞及成熟卵細胞,各期卵細胞的形態結構特點及各部分結構的變化情況。There are five developmental stages in the spermatogenesis, namely are primary spermatogonium, secondary spermatogonium, primary spermatocytes, secondaty spermatocytes and spermatids. the spermatids develop into sperm via the process of spermiogenesis. there exist cell junctions between two spermatids
電子顯微鏡觀察表明:大眼鱖的精子發生和形成過程,經歷了初級精原細胞,次級精原細胞,初級精母細胞,次級精母細胞和精子細胞階段。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母, g418篩選出多拷貝插入的單克隆,甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白,培養液上清經sds - page電泳分析,在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ,能與羊抗hcmv多克隆抗體發生發應的條帶。Spermal ultrastructure the sperm ultrastructure of the macrobrachium rosenbergii and exopalarmon carinicanda holthuis analogical, the whole sperm looks like a inversed umbrella, it has a main body, a spike and a little bit cytoplasm. there are some vesicle exist in the m. rosenbergii sperm nucleus. the relatively length of the spike of the e
各種細胞器數量在精子發生過程中逐漸增多,其中內質網以粗面內質網為主,線粒體于初級精母細胞階段始出現,膜輪結構在次級精母細胞中出現,精細胞早期的上述細胞器分化成電子密度高的前頂體腔,並逐漸分化形成方形頂體。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。分享友人