病毒中和反應 的英文怎麼說

中文拼音 [bìngdúzhōnghéfǎnyīng]
病毒中和反應 英文
virus neutralization reaction
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 和 : 和動詞（在粉狀物中加液體攪拌或揉弄使有黏性） mix （powder） with water, etc. : 和點兒灰泥 prepare some plaster
  • 反 : Ⅰ名詞1 （方向相背） reverse side 2 （造反） rebellion 3 （指反革命、反動派） counterrevolutionari...
  • 應 : 應動詞1 （回答） answer; respond to; echo 2 （滿足要求） comply with; grant 3 （順應; 適應） suit...
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. In this experiment, seedlings of arabidopsis thaliana ( col ) were observed after being treated by verlicillium dahliae ( vd - toxin ), exogenous salicylic acid ( sa ), nitric oxide donor ( snp ) and nitric oxide synthase inhibitor ( nna ), then we investigated the changes of endogenous h2o2 content, the activity of the antioxidant enzymes catalase ( cat, ec : 1. 11. 1. 6 ) and ascorbate peroxidase ( apx, ec : 1. 11. 1. 11 ) and mrna levels of cat3 in different stress conditions, we also identified the localizations of h2o2 and no accumulated in the leaves of arabidopsis

    本實驗研究了棉花黃萎病菌?大麗輪枝菌毒素（ vd - toxin ）與擬南芥幼苗互作反應中外源sa 、 no供體snp 、 no合酶抑制劑nna等不同處理對擬南芥幼苗h _ 2o _ 2含量、 cat和apx活性及cat基因mrna表達量的影響，並對no 、 h _ 2o _ 2的積累部位進行染色檢測。
  3. The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a

    其次，通過氨基酸序列和結構的比較，確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能；然後，應用重組dna技術，互換tm - 2 ~ 2基因和tm - 2基因的對應結構域，構建嵌合基因，獲得嵌合蛋白表達的轉化體，驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。
  4. Iraq ' s procurement efforts include equipment that can filter and separate micro - organisms and toxins involved in biological weapons, equipment that can be used to concentrate the agent, growth media that can be used to continue producing anthrax and botulinum toxin, sterilization equipment for laboratories, glass - lined reactors and specialty pumps that can handle corrosive chemical weapons agents and precursors, large amounts of vinyl chloride, a precursor for nerve and blister agents, and other chemicals such as sodium sulfide, an important mustard agent precursor

    伊拉克采購的設備包括可過濾和分離生化武器中微生物和毒素的設備；可用於為炭疽病毒和肉毒（桿）菌病毒集中藥劑和生長媒體的設備；實驗室殺菌設備；可處理腐蝕性化學武器藥劑、前體、乙烯基氯化物（一種神經和水泡藥劑）及其他化學藥劑（如鈉硫化物，芥子氣藥劑的前體）的玻璃線紋反應堆和專業水泵。
  5. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  6. On march 8, real - time polymerase chain reaction orthopoxirus generic assay and nonariola orthopoxirus assay were positie for orthopoxirus dna, supporting the clinical diagnosis of e, and the diagnosis of accinia was confirmed at the us centers for disease control and preention

    3月8日，實時聚合酶鏈反應痘苗病毒屬測定和非天花痘苗病毒屬測定痘苗病毒屬dna是陽性，支持牛痘性濕疹的臨床診斷，並且美國疾病控制與預防中心也證實了牛痘的診斷。
  7. On march 8, real - time polymerase chain reaction orthopoxvirus generic assay and nonvariola orthopoxvirus assay were positive for orthopoxvirus dna, supporting the clinical diagnosis of ev, and the diagnosis of vaccinia was confirmed at the us centers for disease control and prevention

    3月8日，實時聚合酶鏈反應痘苗病毒屬測定和非天花痘苗病毒屬測定痘苗病毒屬dna是陽性，支持牛痘性濕疹的臨床診斷，並且美國疾病控制與預防中心也證實了牛痘的診斷。
  8. The two isolates were positive reaction hi polymerase chain reactions with two pair of primers specific for alv - j and gave antigenically strong reaction hi the indirect fluorescence antibody assay ( ifa ) with alv - j specific monoclonal antibody je9. negatively - stained electron microscopic and immune - electron microscopic observation demonstrated that viral particles of the inner mongolia and shandong isolate of alv - j, respectively designated imc10200 and sdc2000 strain of alv - j, showed characteristic morphology of alv

    利用pcr和間接免疫熒光反應進行鑒定，兩株j亞群禽白血病病毒可以被兩對alv - j特異性引物擴增（特異條帶約2 . 2kb和545bp ） ，且在特異性單克隆抗體je9的間接免疫熒光檢測中呈現強陽性熒光反應。
  9. More than 50 cases seeking class - action status have been filed in recent weeks in response to pet illnesses and deaths linked to tainted pet food

    近數周，在人們對寵物食品中毒有關聯的寵物生病和死亡的反應中，有超過50個尋求集體行為的案件被提交。
  10. Producing plant phytoalexins has been recognized as the primary and dominant routines in defending system in cotton, include terpenoid, proteases and hormones in which many enzymes, such as chitinase, has been proved useful against verti - cillium in several plants, but not their functional mechanism

    在棉花抗病反應中以植物抗毒素的合成為主要途徑，包括萜類、酶類和激素類3類化學物質，其中酶類物質如幾丁質酶已經在很多植物中被證明具有抗病作用，但是其具體作用途徑仍不清楚。
  11. Hbv genotype was determined by the restriction fragment length polymorphism analysis in patients with chronic hbv infection in 5 cities of fujian province. 2. the sensitivities and specialties of melting curve assay and pcr microplate hybridization - elisa assay were compared with mpcr - rflp and sequence analysis for the detection of hbv ymdd mutants in 44 serums from patients receiving lamivudine monitherapy with viral breakthrough

    應用熔解曲線法和pcr微板核酸雜交- elisa法對44例接受拉米夫定治療過程中出現病毒學反跳時的血清進行ymdd突變株的檢測，並與測序法和mpcr - rflp法比較它們的敏感性、一致性。
  12. No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract

    將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。
  13. By 2005, strengthen the response to hiv / aids in the world of work by establishing and implementing prevention and care programs in public, private and informal work sectors and take measures to provide a supportive workplace environment for people living with hiv / aids

    到2005年，增強在工作環境中對艾滋病病毒/艾滋病的反應力度，在公共、私有和非正式工作部門中建立和實施艾滋病預防和護理計劃，並採取措施，在工作場所中為艾滋病病毒攜帶者/患者營造支持性環境。
  14. Aids is a severe immunological disorder caused by the retrovirus hiv, resulting in a defect in cell - mediated immune response that is manifested by increased susceptibility to opportunistic infections and to certain rare cancers, especially kaposi ' s sarcoma

    愛滋病是一種由逆轉錄酶病毒引起的嚴重免疫系統混亂，導致細胞中介免疫反應的缺失，表現為容易受感染和受某些罕見癌癥的侵襲，尤其是卡波濟氏腫瘤。
  15. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  16. The farms have been quarantined after four dead chickens and 14 swab samples tested positive for h5 avian influenza virus in the past two days. " the two farmers culled all chickens in the sheds where the dead chickens were found

    署方過去兩日從雞場抽取樣本進行測試，其中四隻死雞樣本和十四個雞糞樣本對h5禽流感病毒呈陽性反應，隨即查封該兩個雞場。
  17. Bleeds were taken from all mice 2 weeks after each immunization. then we assayed the influenza virus specific antibody and the ratio of igg1 to igg2a in mice serum

    每次免疫后尾部采血，檢測血清中的流感病毒特異抗體和抗體中的iggi / iggza比值，以確定免疫反應的主要類型（ thz / thl ） 。
  18. As the first tertiary treatment works in hong kong to produce reclaimed water, the ngong ping sewage treatment works uses a sequencing batch reactor, dual media filter and disinfection process to reduce organic pollutants, suspended solids, nutrients and pathogenic organisms from sewage to a very low level

    昂坪污水處理中心是香港首間處理再造水的三級污水處理廠，採用生物反應池、雙濾層三級濾池和消毒程序，將污水中的有機污染物、懸浮固體、營養物以及病原微生物減至極低水平。
  19. Methods : extracting the total rna of human pbmc, the objective include 60 healthy blood donator, 30 patient with viral encephalitis and multiple sclerosis and parkinsonian syndrome, 30 patient with schizophrenia and affective disorder, this indviuals were inpatients or outpatients of the first hospital of chongqing university of medical science from december, 2000 to june, 2001. using nested rt - pcr techique to detect borna disease virus " middle fragment in orf i, and using southern blot hybridization to analyze the pcr product

    重慶醫科大學碩士學位論文方法：提取從2000年12月至2001年6月在重慶醫科大學第一附屬醫院神經科和精神科住院及門診的60例健康獻血者、 30例包括原因未明的病毒性腦炎、多發性硬化、帕金森綜合征，以及30例包括精神分裂癥和情感障礙患者pbmc中的總kn 』 a ，採用套式逆轉錄聚合酶鏈反應estedrticr ）技術進行了bdvorf基因中部片段的檢測，並對pcr產物進行電泳分析及southernblot雜交分析。
  20. The deduced amino acid sequence revealed that the isolates from guangdong province possibly were the primary source of afvs of mainland china. the central china area, such as henan province, may be a secondary source to the north and west of china. atvs changed by and by in the south district of mainland china, just like nan jing and shanghai

    本實驗將在中國大陸1994年至2001年期間，不同地區、不同年代和不同宿主中分離到的h9n2亞型禽流感病毒株，應用逆轉錄聚合酶鏈反應rt - pcr擴增出該亞型病毒的血凝素基因ha ，經測序，獲得的30株中國大陸h9n2亞型禽流感病毒分離株的核苷酸序列。
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