病毒質體 的英文怎麼說
中文拼音 [bìngdúzhítǐ]
病毒質體
英文
viroplast- 病 : Ⅰ名詞1 (疾病; 失去健康的狀態) illness; sickness; disease; malum; nosema; malady; morbus; vitium...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 質 : Ⅰ名詞1 (性質; 本質) nature; character; essence 2 (質量) quality 3 (物質) matter; substance;...
- 體 : 體構詞成分。
- 病毒 : [醫學] virus; inframicrobe (濾過性)
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The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。The fourth types are stract cells, where endoplasmic reticulums showed concentric circle
病毒包涵體位於細胞質內,有兩種類型。Interferon is human body gets virus or double stranded the exciting generation immunity of rna exciter is respondent, by the cell synthesis reachs excretive gens protein kind
干擾素是人體受到病毒或雙股rna刺激物的刺激產生免疫應答,由細胞合成及分泌的一族蛋白質類。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。It was important glucoprotein modulating cell functions by combination to idiosyncrasy receptor on cell membrane, which inducing process of cascade signal magnifying, then passing signals into nucleus to induce the modulation of the serial gene expression and viarous physiological reaction, and inhence immunity and antivirus
人-干擾素( hu - - ifn )是人體因病毒感染或其它誘生劑作用等所產生的一類非特異性抗病毒物質,是調節細胞功能的一類重要的糖蛋白。The results showed that the number of polyhedra obtained by the staining method was less than that by hemacytometer, but the coefficient of variation of the samples was lower than that of hemacytometer method, thus the staining method can be used in quality control test of virus insecticides
該染色計數法測得的多角體數量要低於血球計數板的計數結果,但變異系數較血球計數板計數法顯著較小,結果穩定,適用於病毒殺蟲劑的質量檢測。Water quality - detection of human enteroviruses by monolayer plaque assay
水質.用單分子層噬菌斑序列檢測人體腸病毒The pathogen of goose plaque, goose parvovirus ( gpv ), can cause acute infectious disease characterized by acute intestitis and inflammation of liver, kidney and heart in goslings and muscovy duckings under 1 month of age, which threaten the goose industry
鵝細小病毒( gooseparvovirus , gpv )為小鵝瘟的病原體。小鵝瘟主要發生於1月齡內雛鵝和雛番鴨,是以急性腸炎及肝、腎、心實質臟器炎癥為特徵的烈性傳染病,對養鵝業發展造成很大威脅。T1 phage displayed a higher survival than poliovirus.
噬菌體的存活高於脊髓灰質炎病毒。The soil salification is a serious problem for agriculture and environment. especially now with the development of industry, the situation of soil salification becomes more and more serious, and the quality of water is continuous worsened. research on the mechanism of salt tolerance especially in the important crops, such as wheat, is becoming more urgent than ever before
土壤鹽漬化是影響農業生產和生態環境的嚴重問題,隨著工業污染加劇,灌溉用水的質量不斷下降和化肥使用不當等原因,次生鹽堿化土壤面積有不斷加劇的趨勢,給農業生產造成重大損失;黃矮病毒病是小麥等禾本科植物的重要病害之一,其傳毒介體蚜蟲更使植物產量損失巨大。" viruses, plasmids, and transposable genetic elements. " chap 6. in molecular biology of the cell. pp. 273 - 8
細胞內的分子生物學第六章病毒、質體,與轉位基因元件。第273到278頁。Differential diagnosis included cerebral toxoplasmosis, cytomegalovirus ( cmv ) encephalitis, primary cns lymphoma, progressive multifocal leukoencephalopathy, fungal abcess due to candida, aspergillus, or cryptococcus, varicella - zoster virus encephalitis or vasculitis, herpes simplex encephalitis, tuberculosis ( m. tuberculosis ), and kaposi ' s sarcoma
其它的診斷包括:腦弓形體病,巨細胞病毒( cmv )型腦炎,原發中樞系統淋巴瘤,漸進性多灶性腦白質病,假絲酵母菌,麴菌或隱球菌所致真菌性膿腫,水痘帶狀病毒型腦炎或脈管炎,單純皰疹腦炎,肺結核(多發性結核) ,和卡波西肉瘤。Construction of recombinant adeno - associated virus vector expressing glial cell line - derived neurotrophic factor labeled by green fluorescent protein
綠色熒光蛋白標記的大鼠膠質細胞源性神經營養因子重組腺病毒載體的構建及其表達Furthermore, we discussed the influence of immune - sensibilization before induction to the activity of t - cell vaccine, compared the immune - sensibilization difference between hcv - adenovirus vectors and plasmid vectors
此外,我們還探討了誘導前免疫致敏對t細胞疫苗活性的影響,並比較了hcv腺病毒載體和質粒載體在此免疫致敏功能方面的差異。Expressive efficiency of recombinant adenovirus vector of human osteoprotegerin gene in bone marrow stroma cell in rats
人骨保護素基因重組腺病毒載體在大鼠骨髓基質細胞中的表達效率4 we observed that t cell vaccine activity could be greatly improved through immune - sensibilization to mice with hcv adenovirus vectors or plasmid vector before t - cell vaccine induction, and adenovirus vectors proved to be superior to plasmid vector
在誘導t細胞疫苗之前,用v腺炳毒載體或質粒載體討小鼠進廳免疫致敏,可以明顯提高t細胞疫苗的活性;其中腺病毒載體的免疫致敏效果要明顯好於質粒載體。Pad. hcv - c, pad. hcv - cel and pad. hcv - ce ! e2 were packed and screened by virus plaques technique, identified to be good infectivity and expressivity of inserted hcv structural proteins or fusional structural. in contrast to plasmid dna vector, adenovirus vectors appeared to be better immunizational effects and with the hope of being developed to be vaccine against hcv
Hcv ceiez ,經鑒定具有較好的感染性,能較好的表達插入的hcv結構蛋白或融合結構蛋臼,與質粒dna載體相比,腺病毒載體具有更好的免疫效果,可望發展成為抗hcv感染的腺病毒載體疫苗。( 3 ) establishing a new method which can quantitatively determinate telomerase activity. 1. human telomerase rna component ( htr ) gene was cloned from hela cells using gene engineering technology. the htr gene was then reverse inverted into retrovirus vector plncx and constructed an antisense recombinant plasmid plncx - atr
將htr基因插入逆轉錄病毒載體plncx中,構建了htr基因的反義重組質粒plncx - atr和正義重組質粒plncx - tr ,測序結果表明符合預期要求,為下一步實驗奠定了基礎。We constructed 3 kinds of shuttle plasmid encoding hcv structural proteins by means of molecular cloning technique ; based on it, 3 kinds of adenovirus vectors of hcv structural proteins were packed, screened and identified. we also selected and synthesized 5 kinds of ctl epitope peptides of hcv structural gene which induced 5 kinds of corresponding hcv specific ctl, which were evaluated by experiments in vivo and in vitro
本研究利用分子克隆技術,構建了三種hcv結構蛋白腺病毒載體穿梭質粒,並以此為基礎包裝、篩選和鑒定了三種hcv結構蛋白腺病毒表達載體,選擇併合成了hcv結構基因區5條ctl表位多肽,並以此誘導了5種hcv特異性ctl ,並對其進行了體內外的實驗測定。Hiv antibody testing : recommended measures to generate quality results 1994
提供高質素的愛滋病病毒抗體測試的建議1994年只備英文版分享友人