病毒基因重組 的英文怎麼說

中文拼音 [bìngdújīyīnzhòngzǔ]
病毒基因重組 英文
viral recombination
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  • 重組 : bpr
  1. Construction and identification of recombinant adenovirus of mouse noggin gene

    基因的重組腺病毒構建與鑒定
  2. Expression and genetic immunization of hantaan virus g2 recombinant adenovirus

    2重組腺病毒的表達及其基因免疫的研究
  3. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. Reactivation between 2 related viruses is the recombination of genome between an active virus and an inactivated virus.

    在兩種有關的病毒之間出現的復活，是一種活性病毒與一非活性病毒之間發生了基因組重組的結果。
  6. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  7. The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a

    其次，通過氨基酸序列和結構的比較，確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能；然後，應用重組dna技術，互換tm - 2 ~ 2基因和tm - 2基因的對應結構域，構建嵌合基因，獲得嵌合蛋白表達的轉化體，驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。
  8. The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr, and express by protokaryotic and eukaryotic expressing system, then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine

    利用pcr方法擴增和克隆gpvh1分離株主要免疫原性蛋白基因vp3 ，並對其進行原核和真核表達，是建立小鵝瘟分子診斷方法、構建vp3基因重組禽痘病毒活載體疫苗的基礎，具有極為重要理論和實踐意義。
  9. Preparation and identification of recombinant adenoviral vectors containing human wild type spk and its mutant gene

    攜帶人鞘氨醇激酶及突變體基因重組腺病毒載體的制備及表達
  10. The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus

    本研究的目的在於以人的-干擾素基因（ ifn - ）作為目的基因，與鯉魚-肌動蛋白基因啟動子在體外重組，利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究，其結合了干擾素和基因工程育種抗草魚出血病病毒的優點，以期獲得對草魚出血病具有天然抗性的轉基因魚，並在此基礎上培育出草魚抗病新品系。
  11. The result shows that a vvibdv strain was obtained, the above work lay a important role for further studying on the molecular biological mechanism of antigenic drift and virulence variation of ibdv, molecular epedimiology, it also provided the basis for recombinant and gene deleted vaccine of ibdv

    本實驗可以幫助我們進一步探討ibdv抗原性漂移和毒力變化的分子生物學機制，追溯ibdv的起源，理解病毒的傳播方式。同時也為研製開發基因重組疫苗和缺失疫苗打下一定的基礎。
  12. Studies on the inherent stability of recombinant fowl pox virus expressing glycoprotein b of infectious laryngotracheitis virus

    基因重組雞痘病毒疫苗的遺傳穩定性評價
  13. Effects of reticuloendotheliosis virus infection on ifn - production in the splenocytes of spf chickens

    利用基因重組技術將雞傳染性支氣管炎病毒
  14. Expressive efficiency of recombinant adenovirus vector of human osteoprotegerin gene in bone marrow stroma cell in rats

    人骨保護素基因重組腺病毒載體在大鼠骨髓基質細胞中的表達效率
  15. It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified

    中國豬瘟兔化毒（脾淋毒）基因組cdna文庫的構建、序列分析：根據已發表的豬瘟病毒（ csfv ）核苷酸序列，藉助計算機軟體分析，選擇高保守區段和基因組中的單一限制性酶切位點，利用rt - pcr及nested - pcr和helf - nestedpcr技術，成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ，分別克隆到pmd - 18t或pgem - teasy載體進行測序后，拼接出了其核苷酸序列。
  16. To solve this problem, we used bac - to - bac system to recombine gfp - actin fusion gene and gfp gene under the control of the promoter of polyhedrin gene respectively. two recombinant virus : acmnpv - gfp - actin which contains gfp - actin fusion gene and acmnpv - gfp which contains gfp gene were obtained. the latter was set as a control

    為了解決以上問題，在以上實驗的基礎上，我們利用bac - to - bac系統分別將gfp基因和gfp - actin融合基因重組于多角體基因啟動子之下，構建了兩種重組病毒，即含gfp - actin融合基因的重組病毒acmnpv - gfp - actin和含gfp基因的重組病毒acmnpv - gfp 。
  17. Dr tsang said that hong kong has a very comprehensive avian influenza surveillance programme to detect the presence of any avian influenza in our environment and the possible reassortment of the viruses so that prompt responsive measures can be taken

    曾醫生說香港有全面的禽流感監察計劃，監察環境中的禽流感，及病毒會否出現基因重組，以採取迅速的應變措施。
  18. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
  19. The polymerase is crucial in this process because it copies the iral genome and directs the production of its proteins.

    在這個過程中多聚酶是至關重要的，因為它復制病毒基因組並且指導其蛋白質的合成
  20. Pbluebachisc - vp7 was identified and analped by compnding restriction endonuclease, pcr and nested pcr on the basis of the genetic sites of pbluebachisc, which was idenhfied as vp7 gene of prv hafied pbluebachisc - vp7 and barmidn - blue dna were cbeinfected insect cell sro and harvested mmbinan beculovirus 5d later identification with restriction empme and pcr showed vp7 gene has been arembwt comehy with baculovirus dna and namd a - l

    純化該重組質粒並與線性桿狀病毒dnabac - n - blue共轉染昆蟲細胞sr9 ， 5d后收獲重組病毒。重組桿狀病毒dna分子的pcr及酶切鑒定表明，獲得了prv - vp7基因與桿狀病毒dna的重組子，命名為a - 1代病毒。
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