盒中粒子 的英文怎麼說
中文拼音 [hézhōnglìzi]
盒中粒子
英文
particle in a box-
Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。We amplified ppsa and tkta from e. coli k - 12 by pcr and constructed recombinant plasmids of them in pbv220 vector containing prpl promoter. because of each gene carrying pl promoter, four productions of ligation were obtained, that are ppt -, ppt -, ptp -, and ptp -. the results of sds - page demonstrated that the bands at 84kd and 73kd were more intensive than the same ones of the controls
3 .從大腸桿菌中克隆了莽草酸途徑關鍵酶基因aro刁、 aroc 、 arob及整體代謝調控基因csra及其側翼序列;從質粒pet28a中克隆了kan抗性基因; 4 .構建了基因盒子aroaar口carobkan ，在其前面加入了強啟動子ptac ，初步證明了pta 。
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