真核載體 的英文怎麼說

中文拼音 [zhēnhézǎitǐ]
真核載體 英文
eukaryotic vector
  • 真 : Ⅰ形容詞（真實） true; genuine; real Ⅱ副詞1 （的確; 實在） really; truly; indeed 2 （清楚確實） cl...
  • 核 : 核構詞成分。
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  2. Cloning and sequecing analyzing of the hemagglutinin gene from influenza a h3n

    基因真核表達載體的構建及序列分析
  3. We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0

    我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ，然後利用脂質體轉染家蠶bmn細胞，瞬時表達hgh 。
  4. On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection

    本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。
  5. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  6. ( genbank submission no. ay190323 ) eukaryotic recombined expression vector, pcdna3

    1和pegfp上，獲得真核重組表達載體pcdna3
  7. The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr, and express by protokaryotic and eukaryotic expressing system, then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine

    利用pcr方法擴增和克隆gpvh1分離株主要免疫原性蛋白基因vp3 ，並對其進行原核和真核表達，是建立小鵝瘟分子診斷方法、構建vp3基因重組禽痘病毒活載體疫苗的基礎，具有極為重要理論和實踐意義。
  8. We successfully construct the eukaryotic expression vector of gfp - eif - 5a and its mutational vector using genetic engineering techniques. we found that eef - 5 a localized in nucleus as well as in cytoplasm just for a short time after its transient expression, then distributed only in cytoplasm

    Eif - 5a的hypusine修飾是其活性和功能發揮所必需的，我們通過pcr方法實現了hypusine位點的定點突變，並進一步構建了含gfp標簽的eif - 5a及其hypusine位點突變的真核表達載體。
  9. ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed

    （ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。
  10. Studies on construction and immunity enhance of double expression plasmids of classical swine fever virus e

    2基因真核雙表達載體的構建及其免疫增強作用的研究
  11. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  12. The modified sequence was cloned into binary vector pbi121. the constructed expression vector was named pbi121 - bar, then transferred into agrobacterium tumefaciens lba4404 by triparental crossing. thus the project bacterium has been successfully constructed

    改造后的目的基因克隆于真核表達載體pbi121 ，構建表達載體pbi121 - bar ，通過三親雜交法將真核表達載體pbi121 - bar轉入農桿菌lba4404 ，成功構建出工程菌。
  13. Therefore, we hope to construct a effective eukaryotic gene expressing vector harboring a genomic dna, including introns, and develop a gene expressing system could correctly splice the mrna

    為此，我們希望構建和探索一種能有效高表達含有內含子真核基因組的載體和能對內含子進行剪切加工的昆蟲表達系統，以提高外源真核基因表達的有效性和可靠性。
  14. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  15. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
  16. They cannot naturally produce essential fatty acids - beneficial nutrients found mainly in plant and fish oil, so they must rely on a dietary supply. here we want to establish transgenic mice carry a tissue - specific expression gossypium hirsutum - 6 fatty acid desaturase ( fad2 ) gene which can add a double bond into an monosaturated fatty - acid hydrocarbon chain and convert oleic acid to linoleic

    本試驗試圖構建一個肌肉組織特性的- 6脂肪酸去飽和酶（脫氫酶）真核表達載體，通過原核顯微注射法把棉花的- 6脂肪酸去飽和酶導入到小鼠體內，以小鼠為動物模型，建立動物體內的必需脂肪酸合成代謝通路。
  17. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  18. Construction of recombinant retroviral vectors expressing b7 - 1 gene in cell

    1基因的重組逆轉錄病毒載體構建及真核表達
  19. Construction of the eukaryotic expression vector for the gene encoding the 26 kda outer membrane protein of helicobacter pylori and identification of the expressed proteins

    000外膜蛋白真核載體的構建及表達蛋白的鑒定
  20. Construction of a sv40 virus large j antigen eukaryocyte vector and its targeted expression

    抗原真核載體的構建與表達
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