真菌培養基 的英文怎麼說
中文拼音 [zhēnjūnpéiyǎngjī]
真菌培養基
英文
fungous medium- 真 : Ⅰ形容詞(真實) true; genuine; real Ⅱ副詞1 (的確; 實在) really; truly; indeed 2 (清楚確實) cl...
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
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Isolate all grew well in the culture medium with initial ph 4 - 10, the optimal growth temperature range was from 28 to 30. 5 degree c. it grew well on the medium for fungi growth, such as pda medium and czpek medium etc, and also grew well on the cause ' s i medium and the non - nitrogen medium, but little growth on the luria bertani medium ( lb ) and beef extract peptone medium. it did not need special nutrition factors for growth, but source of the carbon was the key factor to growth, all of its nutrition needs were different from that of common bacteria
該菌在初始ph4 - 10的培養基中都能夠生長,生長最適溫度范圍為28 - 30 . 5 ,在pda 、查氏等真菌培養基中生長旺盛,在高氏1號和無氮源培養基中同樣生長良好,而在lb與牛肉膏蛋白腖等細菌培養基中生長很差,碳源是其生長的關鍵因子,這有別於一般細菌的營養需求。Mycelial growth and exo - biopolymer production by submerged culture of various edible mushrooms under different media
中譯:數種可食用真菌于含不同媒介之液態培養基中的菌絲體生長與外生物大分子的產生。His observations of fungi in their natural habitat and his methods of culturing them enabled him to work out their developemt and life cycles, thus laying down the foundations of modern mycology
他在真菌的天然棲息地觀察真菌,並通過培養真菌的方法來獲得有關它們發育和生活史的相關知識,繼而建立了現代真菌學的基礎。Potato dextrose agar and grain medium were also used to identify fungi which were not determined by the primary culture. fungi were all secondarily cultured on sabouraud medium to observe the colony ' s texture, colour, growth rate, surface status and reverse pigment. the fungi should be examined by microscope to inspect their microscopic structure from 7th day to 21st day
使用的培養基有沙氏培養基、土豆培養基、真菌試驗培養基和5種種子培養基,連續培養4周,並隨時觀察菌落的色彩、生長速度、表面狀態、背面顏色等,並從第7天?第21天連續鏡檢以檢查真菌的顯微結構,綜合菌落形態和顯微結構,以確定真菌的種屬。After inoculation, all strains were examined by microscopy, hyphae or cells of all strains were observed, but none was seen in negative comparison. in the same time, originally fungi were isolated again in sabourud which showed that these fungi could grow and reproduce in these animals, but if they can cause infection or not will be make sure with impressionable animals
回接后所有實驗菌種在顯微鏡下均可見有菌絲或菌細胞生長,而陰性對照組則未見生長,同時可以從沙堡氏培養基中再次分離得到該菌,說明這些菌種均可以在動物體內生長繁殖,但是否能真正引起感染,還需要進一步使用易感動物進行確認。The v. dahliae and other fungal pathogens in cotton were tested after the positive clones were further incubated. the results showed that the acquired monoclonal antibodies could identify v. dahliae kleb. with other pathogens at specie level
經擴大化培養后取上清液對棉花上常見的病原真菌及棉花大麗輪枝菌其他若干菌系進行鑒定,結果表明,所制得的單抗基本上能將棉花黃萎病菌鑒定到種。Culture medium a mixture of nutrients used, in liquid form or solidified with agar, to cultivate microorganisms, such as bacteria or fungi, or to support tissue cultures
培養基:以液體或添加瓊脂的固體形式人工配製的適合微生物,如細菌或真菌以及組織培養生長要求的混合營養物質。Had the highest activities of xylanase and cmcase among all isolates. with rice straw as fermentation substrates, the maximum activities of xylanase and cmcase by a4 were 13. 54 u ? ml - 1, 0. 25 u ? ml - 1, respectively
第二部分對培養基底物及主要組分無細胞瘤胃液和酵母膏對厭氧真菌a4菌產酶的影響進行了研究。The reduced level of cell - free rumen fluid had no significant effect on xylanase production, but had significant effect on the cmcase activity. without cell - free rumen fluid, the high concentration level of yeast extract could improve xylanase and cmcase production. in the third section, crude enzymes produced by anaerobic fungus a4 was extracted, and their characteristics of the crude enzyme was also investigated
與基礎產酶培養基相比,降低培養基中無細胞瘤胃液濃度對厭氧真菌所產木聚糖酶的酶活及比活力無顯著影響( p 0 . 05 ) ,但對其所產的羧甲基纖維素酶的酶活及比活力有顯著影響( p 0 . 05 ) 。In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia
並以已構建的載體ptyb2 - mae為模板,通過pcr擴增出融合基因mae - imein - cbd ,將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。Effect of medium components on enzyme production and characterization of anaerobic fungal crude enzymes were also investigated. this thesis was described in the following three sections. in the first section, twelve anaerobic fungal strains isolated from rumen and faeces of ruminants were screened for xylanase and cmcase production
本研究從黑白花種公牛、水牛、山羊糞樣及山羊瘤胃內容物中分離到12株厭氧真菌,並對其進行了產高活性羧甲基纖維素酶和木聚糖酶菌株的篩選,同時還就培養基主要組分對厭氧真菌產酶的影響和厭氧真菌的粗酶性質進行了研究。It produced strong antifungal bioactive substance on medium containing 4 % of starch, 0. 5 % of yeast extract and 75 % of stale seawater cultured at 28 for 96h
海洋微生物菌株041381利用澱粉培養基(澱粉4 ,酵母膏0 . 5 ,陳海水75 ) ,在28下靜置發酵培養96h產抗真菌活性物質。In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k
本文根據genebank登錄的氨基酸序列,同時考慮構建和表達的需要,化學合成了misgurin基因和接頭,採用一種新的策略,在體外將基因多拷貝同向串連,並將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象,選用真核表達質粒pci - neo ,根據已構建好的含54位密碼子突變型mbl基因t載體的結構,設計合成新的引物, pcr擴增54位密碼突變型mbl基因,凝膠回收,雙酶切pcr產物和pci - neo質粒, t4連接酶連接,將前者克隆至後者的sma和sal位點,轉化大腸桿菌xl - 1blue ,氨芐選擇培養。分享友人