睪丸引帶 的英文怎麼說
中文拼音 [wányǐndài]
睪丸引帶
英文
chorda gubernaculum-
On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction
本實驗在合成該蛋白基因上下游引物的基礎上,利用pcr技術,從人睪丸cdna庫中釣取目的基因,並克隆于pgem - t載體中,經序列測定證明與文獻報道基本一致,再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中,轉化宿主菌,經溫度誘導后,進行sds - page電泳分析,發現在約21kd位置上出現了一條明顯的蛋白帶,與預期相符。
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