端粒和端粒酶 的英文怎麼說

中文拼音 [duānlìhéduānlì]
端粒和端粒酶 英文
telomere and telomerase
  • 端 : Ⅰ名詞1 （東西的頭） end; extremity 2 （事情的開頭） beginning 3 （門類; 方面） item; point 4 （原...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 和 : 和動詞（在粉狀物中加液體攪拌或揉弄使有黏性） mix （powder） with water, etc. : 和點兒灰泥 prepare some plaster
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 端粒酶 : telomerase
  1. The capybara, nearly the size of a grown human, was not expressing telomerase, suggesting evolution was willing to forgo the benefits in order to reign in cancer

    幾乎和成人體重相當的水豚並不表達端粒酶，說明進化還是更樂于放棄復原的益處而到達對癌癥的控制。
  2. Results in the lung cancer group, the positive rate of telomerase activity in surgically resected lung cancer tissues was 91. 7 ( 33 out of 36 samples ), and 85. 7 ( 6 out of 7 samples ) and 71. 4 ( 5 out of 7 samples ) in fiberobronchoscopically collected tissues or cells and pleural effusion cells, respectively

    結果肺癌組： 36例手術切除肺癌組織中端粒酶陽性率為91 . 7 ， 7例纖維支氣管鏡活檢肺癌組織和7例癌性胸水細胞中端粒酶陽性例數分別為6例和5例；總檢出率為88 （ 44 50 ） 。
  3. Telomerase is a ribonucleoprotein complex ( rnp ) composed by its rna component and protein subunits. telomerase can synthesize telomeric dna onto chromosomal ends using its own rna component as a template, elongate the length of telomere, increase cell life and even induce cell immortalization

    端粒酶（ telomerase ）是由端粒酶rna和蛋白質組成的一種核糖核蛋白復合物（ rnp ） 。端粒酶含有引物特異識別位點，能以自身rna為模板，逆轉錄合成端粒dna並加到染色體末端，使端粒延長，從而延長細胞的壽命甚至使其永生化。
  4. With different amount shrnas transfected, in different time, we assayed the telomerase activity of the transfected cells with trap - silver staining and pcr - eia methods

    用trap -銀染法和pcr - eia分別檢測經轉染不同shrna量和作用時間hela細胞端粒酶活性。
  5. The obtained recombinant - 5 - htr plasmid was tranfected into human liver cancer smmc - 7721 cells. all data suggested the expression of plncx - atr could condense cell nucleus and increase nuclear fluorescence intensity, effectively repress the telomerase activity, cell growth and cell proliferation, and induce cell apoptosis

    反義重組質粒plncx - atr轉染人肝癌smmc - 7721細胞，結果發現plncx - atr的表達有效地封閉或抑制肝癌細胞的端粒酶活性，使細胞的生長和增殖受到抑制，細胞體積變小、核緻密、核熒光強度增強，且促進其凋亡。
  6. This paper reviewed the progress of targeted therapy of cancer from points of view of western and chinese traditional medicine and clarified the multiple target effect of chinese materis medica of ant - angiogenesis of cancer, inducing apoptosis and inhibition of telomerase activity

    摘要從中西醫角度分別綜述腫瘤靶向治療的研究進展，闡述中藥在腫瘤治療中的抗腫瘤血管生成、誘導細胞凋亡和抑制端粒酶活性的多靶點效應。
  7. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  8. Comparison of telomerase activity in a few cell lines showed that application of dna sequencer in detecting telomerase enzyme activity is applicable in quantitative and qualitative determination of telomerase activity. the result of this method could be available within 4 - 5 hours with high sensitivity and less handling time. it provided a reliable means to study on regulative mechanism of telomerase activity and relationship between telomerase activation and malignancy

    Trap與dna測序相結合檢測端粒酶活性的方法，操作簡便、快速、經濟、無放射性危害，可在3 ? 4小時內得到檢測結果，大大縮短了檢測時間，為研究端粒酶活性的調節機制、端粒酶與惡性腫瘤之間的關系以及臨床上鑒別和診斷腫瘤的良惡性提供了一種檢測手段。
  9. Purification, identification of human telomerase and its protein subunits

    親和層析法分離鑒定人類端粒酶復合體及其蛋白質組分分析
  10. Expression of telomerase subunits in normal liver tissue and hepatocellular carcinoma tissue

    肝細胞癌組織和正常肝組織中端粒酶亞單位的表達
  11. Effect of adjusted zuojin pills on proliferating cell nuclear antigen and telomerase activity in rats with gastric precancerous lesion

    加味左金丸對大鼠胃癌前病變增殖細胞核抗原和端粒酶活性的影響
  12. Expression of telomerase activity and c - myc mrna in gastrointestinal carcinomas and peripheral tissues

    和端粒酶活性的表達
  13. Effects of starvation on biochemical indexes of blood and telomerase activity of rats

    饑餓對大鼠血液生化指標和端粒酶活性的影響
  14. This thesis mainly focuses on : ( 1 ) effects of the expression of telomerase antisense rna gene on the morphology and biochemical characters of human liver cancer smmc - 7721 cells

    本研究根據端粒酶的結構和功能以及端粒酶的反應特點，從以下幾方面進行了研究。
  15. That is to add a special fluorescence - based dna internal standard in the telomerase elongated ts primers, then do pcr amplification after a step of refinement ( hydroxybenzene / chloroform extracting, and deposited by ethanol ). sequencing analysis of pcr product was done on 310 gene scan analysis ?. 1. 2 dna sequencer to determine telomerase activity. notably, this method eliminated the restraining factors of taq dna polymerase, making it possible to erase the sample differences met in pcr and eradicate the annoying phenomena of pseudo negative results

    在kim等開發的端粒重復擴增分析法（ trap ）的基礎上進行改進，即通過對端粒酶延伸ts寡核昔酸反應產物的精製，消除了pcr擴增中抑制taq酶活性的因素，從而減少了樣品之間pcr擴增上的差異和假陰性現象的發生，提高了判斷樣品端粒酶陰、陽性的準確率和定量的準確性。
  16. This is a very ambitious but potentially far more comprehensive and long - term approach to combating cancer than anything currently available or in development : total elimination of the genes for telomerase and alt ( alternative lengthening of telomeres ) from all of our mitotic cells

    這是一次很有雄心的、但比起目前已有的或正在發展中的與癌癥戰斗任何方法，都潛在地遠為廣泛和長期的方法：從所有我們有絲分裂細胞中全部剔除端粒酶基因和消除alt （端粒變長的另一種途徑） 。
  17. We concluded that excessive expression of exogenous htr gene may compete with the endogenous telomerase rna and prevent rna template from combining with telomeric dna, thus repressing the elongation of telomeric dna ( telomeres ) and causing cell aging and cell death. - 6 - 3. some modifications have been made to overcome the limitation of conventional telomeric repeat amplification protocol ( trap ) assay

    分析其原因，可能是htr基因的過表達在數量和空間效應上同細胞內的端粒酶rna組分產生竟爭，一定程度上阻礙了端粒酶rna模板區與端粒dna的結合，從而抑制端粒dna的延伸，導致細胞凋亡。
  18. All other chemicals were of reagent grade. method : 1. mtt assay : for determining the influence of the medium containing 17 - estrogen or progesterone on cells growth, caov3 were treated with them for 48h and then determined in mtt assay

    2細胞端粒酶制備：將培養的細胞實驗組加人含不同濃度的17p一雌二醇和孕激素的培養液，對照組加同實驗組等量的乙醇門人分別培養12h上4h 8h 。
  19. Results telomerase activity was detected in the lesions of psoriatic patients, but the level of it was lower in comparison with that in tissues of malignant tumor and k562 cell line

    結果銀屑病皮損中有一定水平的端粒酶活性，但其水平顯著低於腫瘤組織和細胞株。
  20. Therefore, a - amylase has been used widely in many industrial fields, such as glucose production, beer brewing, fermentation trade, textile industry, and so on. the study on a - amylase is one of the most active fields in enzyme industrial fields. with the development of biotechnology, more and more scientists and researchers attempt to use dna shuffling technology to breed, screen and even " create " new a - amylase genes with higher activity and other new characters

    設計引物時，上下游引物5 』端分別添加了kpn和bamh酶切位點，克隆得到的基因片段和質粒載體都用kpn和bamh進行雙酶切后，進行酶連、轉化、篩選得到陽性重組子，經過藍白斑驗證、單酶切驗證、雙酶切驗證、 pcr驗證等一系列的驗證后進行測序。
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