篩選得率 的英文怎麼說
中文拼音 [shāixuǎndelǜ]
篩選得率
英文
screened yield-
Through a series of mutagen tests, a mutant strain of monascus spp ( m 12 - 69 ) from ml2 was acquired, which can produce much higher monacolin k than m12, and almost same citrinin level as m12
以之為出發菌株,通過誘變篩選,獲得一株monacolink產率有明顯提高而桔黴素產率僅為0 . 13ng g ( elisa )的突變株,編號為m12 - 69 。In this paper, thirty strains of yeast were isolated from nineteen samples of fermented milk ( ten koumiss samples, three fermented goat milk samples, four fermented milk samples, two shubat samples ) which were collected from the west - centre region of inner mongolia from which we aimed to select the yeast with high vitalistic ? galactosidase. according to the results of dedermination that were the metabolism rate and ratio vitality of ? galactosidase, stk - 1 - 1 has been selected. the result of identification showed that stk - 1 - 1 belonged to kluyveromyces marxianus of kluyveromyces
本研究主要以內蒙古中西部地區牧區採集的19個發酵乳樣品( 10份酸馬奶、 3份發酵山羊奶、 4份酸牛奶和2份發酵駝奶)為研究對象,分離獲得30株酵母菌,從中篩選乳糖酶高活力酵母菌株。根據乳糖代謝率和乳糖酶比活力的測定結果,選出菌株stk - 1 - 1為優勝菌株。經鑒定,該菌株屬克魯維酵母屬( kluyveromyces )的馬克斯克魯維酵母( kluyveromycesmarxianus ) 。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明:運用msha法檢測1型菌毛的檢出率為80 ( 36 45 ) , pcr法的檢出率為95 . 5 ( 43 45 ) , pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強;選擇5株優勢血清型雞源致病性大腸桿菌代表株( o _ ( 89 ) , o _ ( 119 ) , o _ ( 141 ) , o _ ( 127 ) )的1型菌毛pila基因的pcr擴增片段經純化后,分別定向克隆到puc18質粒的多克隆位點,構建了含有目的基因片段的克隆質粒,並轉化到dh5株大腸桿菌載體菌中,篩選獲得陽性克隆菌株。Improved hand picking technology greatly enhance the mineral processing tate of waste in many dressing plants. the flotation in normal temperature is a big breakthrough in mineral processing technology of scheelite. improved jig sizing applied in roughing and grqvity dressing, and improved tungsten fine deposit recovering technology enhance the recovery of tungsten fine deposit
手選工藝的改進,使各選廠的廢石選出率得到大幅度地提高;常溫浮選是白鎢選礦工藝的重大技術突破;推廣並改進動篩跳汰使這在粗、重選中獲得應用;鎢細泥工藝的改進提高了鎢細泥的回收率。In this study a microbial system for biphenyl biodegradation is set up in order to investigate the effects of surfactants on biodegradation of hydrophobic organic compounds. four strains which can grow on biphenyl as the sole carbon and energy sources are selected out, in which alcaligenes eutrophus dj1 and pseudomonas sms02 are chosen as degradation strains. after studing the bioavailability of three nonionic surfactants ( op - 10, tween - 80, and triton x - 100 ), they are added into the biodegradation system of biphenyl
為了考察表面活性劑對疏水性有機污染物生物降解的影響,本論文選擇聯苯作為模擬研究體系,篩選、馴化得到四株可利用聯苯作為碳源生長的菌株;考察了聯苯降解菌株對三種非離子表面活性劑op - 10 、 tween - 80 、 tritonx - 100的生物可利用性;用高效液相色譜法測定了非離子表面活性劑對聯苯的增溶曲線;較系統地研究了加入非離子表面活性劑對聯苯生物降解速率的影響。The co - cultivation had been carried out for 2 ~ 3 days. after that, the transformants were obtained by transferring explants to selection medium containing 75mg / l kanamycin and rooting medium contain - - ing 75mg / l, 100mg / l kanamycin. and its rooted frequency was 82. 5 %
為75mg幾、 100mg l的培養基上進行生根篩選,生根率為82 5 。轉入外源基因的轉化體將會在這一系列篩選過程中發芽、生根,獲得轉基因植株。Twelve primers selected from 100 random primers amplified 254 frangments in 14 species of cymbidium, in which 243 segments are polymorphic. the rate of polymorphism was 95. 7 %. and all the segments were ranging from 200bp to 2200bp
從100個隨機引物中篩選出12個隨機引物,對蘭屬植物14種材料進行隨機擴增,共得到254條帶,多態性帶有243條,多態性百分率為95 . 7 。The library consisted of 1. 3 x 106 clones with an average insert size of about 18kb. the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin. screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr, we got four positive clones
3x10 『個重組噬菌體,插入片段大小約為18kb ,含插入片段的頻率為100隊以中亞濱蓉甜菜堿醛脫氫酶門adh )基因近5 』端的約400hp片段為探針,篩選中亞濱蓉基因組文庫,得到了4個陽性克隆。Two pta - mutants have been selected by using suicide substrate after the mini - tn5 transposon insertion mutagenesis of klebsiella pneumoniae m5al. when used in microaerobic fermentation, the amount of acetate produced by the mutants reduced to less than 50 % of the parent strain, and the yields improved whereas the 1, 3 - propanediol titers and productivities decreased
以klebsiellapneumoniaem5al為出發菌株,用mini - tn5隨機轉座誘變結合自殺性底物篩選的方法得到了兩株產乙酸途徑pta基因缺失突變株xl - 6和xl - 11 ,應用到微氧法發酵中,突變株的乙酸產量為親株的50以下,甘油轉化率有所提高,但1 , 3 -丙二醇濃度和生產強度有所下降。Our experiment indicates : ( 1 ) the optimal concentration of kanamycin for screening torenia fournier regenerated buds was 400 mg / l. the ideal transformation was obtained in the following conditions : the leaf discs were dipped in agrobacterium suspension that od600 was 0. 1 for 10 ~ 20 min ; subsequently cocultivated on the ms solid coculture medium containing 20umol / l acetosyringon for 7 to 8 d at 23, and the induction ratio of regenerated buds was 27. 2 %
研究結果表明: ( 1 )篩選藍豬耳轉化芽的最適卡那黴素濃度為400mg l 。 od _ ( 600 )為0 . 1的菌液濃度菌液浸染葉盤10 20min ,固體共培養基(含20 mol l乙酰丁香酮)上23共培養7 8d可獲得理想的轉化效率,轉化芽誘導率為27 . 2 ; 16h光照, 8h黑暗是較理想的共培養光周期;莖段是較好的轉化受體。It is becoming more and more difficult to discover new pesticides, due largely to the increasing environmental and highly competitive nature of the agrochemical market. in an effort to maximize the chances of discovering new active compounds, the emphasis in recent years has been on increasing the number of compounds tested, both on the primary screens and on the follow - up screens. the objective of this research is to establish a new herbicidal screening method which quick, accurate and simple
農藥的生物篩選是新農藥研究開發中的一個重要環節,由於越來越嚴格的環境要求以及農藥市場競爭的加劇,新農藥的創制變得越來越困難;特別是組合化學在新藥合成中的應用,使得化合物合成的速度大大提高,因此,不斷提高對新化合物篩選的命中率,即增加進入生物篩選的化合物的數量成為發現既對環境安全、又具有卓越性能的新農藥品種的一種有效途徑。分享友人