細胞同步化 的英文怎麼說

中文拼音 [xìbāotóngbùhuà]
細胞同步化 英文
cell synchronization
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 步 : Ⅰ名詞1 （步度; 腳步） pace; step 2 （階段） stage; step 3 （地步; 境地） condition; situation; st...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. However, the advance of intracellular labeling techniques enables us not only to visualize more complete dendritic arbor for qualitative analysis, but also to examine the relation between changes in the dendritic arborization and the evoked fast postsynaptic curents - 3 - ( fpscs ) in the same neurons during the postnatal development the aim of this study was to systematically examine the postnatal changes in the configuration of fpscs evoked by the focal stimulation of the stratum radiatum of the ca1 region, and the relationship between the dendritic arborization and evoked fpscs in the rat hippocampal ca1 pyramidal neurons using whole - cell blind patch recording technique combined with biocytin intracellular labeling during the postnatal development ( postnatal day 2 - 70, p2 - p70 )

    但是，細胞內染色技術的進步使我們不僅能觀察到更完整的樹突分支來用於定性研究，而且也可以在同一神經元上研究在發育過程中樹突分支的變化與誘發的快突觸后電流（ fastpostsynapticcurrents ， fpscs ）之間的關系。因此，本研究應用盲法腦片膜片鉗記錄並結合biocytin細胞內染色方法，對發育過程中（生后2 70天）局部刺激大鼠海馬ca1區輻射層在錐體神經元誘發的fpscs的成分變化，以及ca1錐體神經元的樹突分支與誘發的fpscs的關系進行了較為系統的研究。
  2. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  3. When the cells synchronized at a late cytokinesis stage were treated with w7, the dissolving of the midbody was delayed ; furthermore, we found that the dissociation of y - tubulin from the midbody was also inhibited

    W7處理同步化在胞質分裂後期的細胞，不但使中體解聚發生延遲，而且抑制了y一微管蛋白與中體的解離。
  4. A synchronous mitotic experiment system that was in an unchanged culture condition was established

    建立了多頭絨泡菌自然同步化細胞分裂的實驗體系。
  5. Studies on spermatogenesis and oogenesis in palaemon modestus jiang ye - qin ( chemistry department, huzhou teachers coliege, huzhou 313000 ) palaemon modestus belongs to genus palaemon, family palaemonidae, caridea, natantia, decapoda, class crustacean. lt is a kind of freshwater prawn across china and especially abounds in the taihu lake which is regarded as one of the " three delicacies " of the taiha lake. as for the researches on palaemon modestus, v / e can only refer to spermatogenesis of freshwater shrimp exopalaemon modestus ^ ruang hai - xia et al, 2001 ), studies on reproductive biology of exopalaemon modestus l. the structure and development of the male reproductive system ( huang hai - xia et al, 1999 ) and studies on freshwater prawn in the taihu lake ( yan sheng - liang, 1999 ). on the bases of their researches and with the help of tem, i have made further researches on sperm ultrastructure, spermatogenesis, oogenesis and mature oocyte ultrastructure in palaemon modestus

    秀麗白蝦palaemonmodestus屬甲殼綱crustacea十足目decapoda游泳亞目natantia真蝦部caridae長臂蝦科palaemonidae長臂蝦屬palaemon ，是我國南北均產的淡水蝦，其中太湖產量尤其大，與太湖銀魚、鱭魚並稱「太湖三寶」 。有關秀麗白蝦的研究僅見秀麗白蝦雄性生殖系統的研究（黃海霞等， 1999 ） 、秀麗白蝦精子發生的研究（黃海霞等， 2001 ） 。本人在前人工作的基礎上，利用透射電鏡技術（ tem ）進一步研究了秀麗白蝦精子的形態、結構及精子的發生過程，同時還研究了秀麗白蝦卵的發育過程，從卵原細胞到卵黃發生前的卵母細胞、卵黃發生的卵母細胞及成熟卵細胞，各期卵細胞的形態結構特點及各部分結構的變化情況。
  6. This review focused on the developments of plant synthetic seed technology in the past 20 years, and summarized different items on artificial seeds : the concept, significance, status, application prospect, induction and synchronization of somatic embryogenesis, the way of encapsulating, artificial coat, maturation drying, storage and preservation, etc

    摘要本文綜述了近20年來植物人工種子研究的進展，並對人工種子的諸領域：概念、意義、動態、應用前景、體細胞胚的誘導與同步化、包埋方法、人工種皮、干化、貯藏及防腐等進行了概述。
  7. These interactions are primarily dependent upon the coordinated actions of ovarian progesterone and estrogen, moreover, many other factors, such as growth factors, cytokines, ecm, adhension molecules, oligosaccharides and proteases, regarded as local mediators, endometrium and embryo have also expressed some specific receptors, via intracellular signal transduction chains and express some key genes, making receptivity of the uterus and synchronized development of the embryo to the blastocyst stage

    成功的植入是處于接受態的子宮內膜和具有侵入性的胚胎間的同步協調反應。植入過程受多種生長因子及其受體、細胞因子、粘附分子、蛋白水解酶、寡糖等的精細調控，通過胞內信號轉導及關鍵基因的表達使子宮內膜發生一系列復雜變化。
  8. Anti - if - protein antibodies were used as probes for immuno - fluorescence, and the reactions of them with different parts of the cell were detected, which suggested the possibility of the existence of the intermediate - like filaments in the cytoplasm. those proteins homologous to the antibodies distributed regularly in the protoplasm. to characterize the corresponding proteins, sds - page and immunoblots were utilized. the 21, 23, 33 and 68kd proteins were distinguished among the diverse protein constituents of the cell. some of these proteins also showed the cross - reactivities with anti - if - proteins antibodies derived from higher organisms. these two evidences both contributed to the homology of some proteins in

    以抗中間纖維蛋白抗體作為探針進行免疫熒光實驗，得到細胞內不同部位的陽性反應，暗示原生質中可能存在類中間纖維。這些同源蛋白的胞內分佈具有一定的規律性。進一步採用sds - page和免疫印跡技術研究它們的生化性質，發現4種主要蛋白明顯有別于胞內其他蛋白組分。
  9. The purposes of this experiment were to establish cuture systems of fibroblasts and methods of cell cycle synchronization so as to provide better donor cells for somatic nuclear transfer

    本實驗旨在建立成纖維細胞的培養系統和細胞周期同步化方法從而為體細胞核移植提供良好的供體細胞。
  10. In this report, we mainly covered the following aspects of " tissue organ regeneration and replication in situ " : 1 ) procedures of tissue organd regeneration and replication and replication in clnical practice ; 2 ) the discover and existence of potentiald regenerative cell ( prc ) ; 3 ) the proliferation, differentiation and regeneration law of potential law of potential regenerative cells ; 4 ) study procedure on tissue organ regeneration and replication from prcs in vitro based on the model of full skin organ regeneration in situ after extensive in vitro, set up the method and technology of searching life regenerative substance required in tissue organ regeneration and replication in situ. in this study, first, the whole human body is divided into 206 function units, which are the " tissue organ " in regeneration study. then the histology foundation of tissue organ regeneration and replication in situ is set up. in ordre to prove the existence of the potential regenerative cells and their potential baility and function, we established clinical tracking rechnique of skin organ regeneration in situ ; meanwhile, several tissue organ regeneration and replication in vitro models which represent different kinds of runctions were sucessfully set up, with all these techniques and models, we confirmed : 1 ) the existence, function and ability of pptemtoa regenerative cells ; 2 ) the importance of life regenerative substance ; 3 ) the feasibility of tissue organ regeneration and replication in situ ; 4 ) the big value of tissue organ regeneration and replication in situ in life science and medicine progerss. we also showed the possible foreground of capture cancer with this method and technologh. in this report, nearly 200 photographs of several tissue organ regeneration and replication in situ or in vitro demonstrated the whole process of tissue organ and big organ entities regeneration and replication from cells. the results of tissue organ regeneration and replication in situ mainly include : 1 ) whole skin organ regeneration and replication in situ ; 2 ) gastrointestinal mucosa tissue organ regeneration in vitro ; 3 ) hair follicle tissue organ regeneration in situ or in vitro ; 4 ) never tissue organ regeneration in situ ; 5 ) pancreas tissue organ regeneration and replication in vitro ; 5 ) marrow tissue regeneration in vitro ; 6 ) renal glomerulus and tubule tissue organ tugeneraation in vitro ; 7 ) heart muscle regeneration in vitro, etcl. in order to let more and more people know and understand this technology of tissue organd regeneration and replication in situ, herein, for the first time, we publicize the key points of actualizing this technology. also, we publicized the technology procedures and the frame constitute of life substances. we bilieve this is a big contribution to human science

    本研究報告，重點報道了組織器官的原位再生復制的臨床程序，報道了組織潛能再生細胞的發現和存在，以及該細胞的增殖分化和形成組織器官的變化規律.以燒傷后皮膚組織器官的原位再生復制為模型，研究出了體外組織潛能再生細胞復制組織器官的培養方法;以體外組織器官的復制為模型，建立了尋找原位組織器官再生復制所需生命物質的方法和技術.本研究，首先按人體的器官功能，分解為206個功能單位，確立了所復制的人體器官中的組織功能單位為組織器官，從而建立了原位組織器官再生復制的組織學基礎.為了驗證組織潛能再生細胞的再生潛能，建立了皮膚器官原位再生的實體臨床跟蹤技術，同時又建立了能代表有關器官功能類別的代表組織器官的原位和體外復制模型，以多組織器官的成功復制確定潛能再生細胞的作用，確定生命研究再生物質的重要性，確定組織器官原位再生復制的可行性，確定了組織器官原位再生復制的生命科學研究和醫學進步的重大應用價值，同時展示了用此方法和技術攻克癌癥的前景.本研究報告，以近二百幅多個組織器官原位和體外再生復制的實體圖片，展示了潛能再生細胞復制的組織器官和大器官司實體;展示了細胞再生復制器官的全過程.真實的報告了組織器官原位再生復制的成果.所公布的主要成果為:皮膚器官的原位再生復制;胃腸黏膜組織器官的原位和體外再生復制;毛囊組織器官的原位和體外再生復制;神經組織器官的原位復制;胰腺組織器官的體外復制;骨髓組織的體外復制;腎小球小管組織器官的體外復制;心肌的體外復制等.為了讓更多的人學會和掌握組織器官原位再生復制技術，本報告首次公布實施技術的重要環節和技術流程;首次公布了生命再生物質的框架和組成.作者自費研究成果對人類生命科學的一大貢獻
  11. By the compounds of submandibular gland cells and collagen sponges. we investigate the optimal cell denisity of tissue engineered compound of submandibular gland cells and collagen sponges, the cellular compatibility of tissue engineered compound of submandibular gland cells on the collagen sponges with different porosity and the influence of epidermal growth factor on the adherence of submandibular gland cell to collagen sponge. our studies can primary provide theoretical ground work to form the model in vitro of tissue engineering smg

    在本研究中，以初步探討體外頜下腺細胞與膠原海綿支架相互作用為目的，採用體外分離培養sd大鼠頜下腺細胞，然後接種于膠原海綿支架上體外復合培養的方法；從不同接種細胞濃度對細胞一支架復合物影響，同一接種細胞濃度在不同孔隙率的支架上黏附、增殖的情況及表皮生長因子（ egf ）對頜下腺細胞的促增殖作用，促細胞在支架上黏附等三方面入手，初步研究了頜下腺細胞與膠原海綿相互作用的影響因素，為進一步在體外及體內構建較為理想的組織工程化頜下腺提供理論參數和實驗依據。
  12. The paper adopted the common breeding way by series of mutagen treating to the yeast ' s cell and spheroplast. finally, the excellent mutant 75l 3555 was obtained. its nature of the production was determined and it can product ethanol on the condition of 40 and have high fermentative speed and stable production. on the other hand, the test was made that the best fermentative condition was found. in the malt juice, adding 0. 02 % k + or 0. 03 % a13 + can increase the production strikingly and adding some degree concentration trahalose can increase the production too and cut short the time of taming. in the study of using molasses producting ethanol, we can obtain high productivity contained the following composition : nitrogen source 1. 5 % maize and 0. 075 % ( nh4 ) 2so4

    本實驗以常規的育種方法，通過對酵母細胞懸液和原生質體進行一系列誘變，經過大量的篩選，最終選育出一株耐高溫高產菌株75l _ （ 3566 ） ，並對其進行了生產性能的測定。 75l _ （ 3566 ）在40條件下，濃度為20 bx的麥芽汁發酵液中，產酒率高達11 . 5 ，而且發酵速度快，發酵周期可縮短12h ，其生產性能穩定，可以適應大部分以澱粉質為原料的生產廠家的需要。同時本實驗對75l _ （ 3566 ）進行了發酵條件優化的初步研究。
  13. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  14. In this paper, the effect of nuclear actin on the process of chromosome construction has been studied by utilizing the precise natural synchrous plasmodium of physarum polycephalum, sds - polyacryl amide gel electrophoresis ( sds - page ), western blotting, the cell - free system and optics microscopy. the major results and conclusions are as follows : 1

    本實驗以多頭絨泡菌原質團為材料，採用同步化培養、細胞核提取、 sds - page 、免疫印跡、非細胞體系構建、光學顯微鏡觀察等方法，研究了有絲分裂前期核內肌動蛋白對染色體構建的影響。
  15. In the presence of hx, oocyte maturation to mil and the extrusion of polar body were highly synchronized, the oocytes matured to mil stage were activated in higher rates than oocytes matured without hx, and those oocytes matured exposed to hx for all the 24h showed a high rate ( 55 % ) of spontaneous activation

    Hx存在情況下成熟到m期的卵母細胞排出第一極體時同步化程度高，其孤雌活化能力高於正常成熟卵母細胞， hx作用24h組出現自發激活現象（自發活化比率55 ） 。
  16. In this paper, how to prepare cp i and identify physico - chemical property had been studied, and uva oxidative damage l929 was perliminary discussed. these datas provided theoretic foundation and scientific proof for further research and development of this product. pigskin was used as material

    本文圍繞型膠原蛋白（ cp ）的制備及其理化性質進行研究，同時在其防止長波紫外線（ uva ）對細胞的氧化損傷方面進行了初步探索，為cp的進一步研發提供理論基礎和科學依據。
  17. 3. when the cells were treated with w7, a ca2 + / cam inhibitor, the central spindle assembly was affected, and the abnormal central spindle was formed ; besides, when w7 treatment was applied at an early stage of cytokinesis, the division furrow index would decrease substantially. these results show that cam is really involved in the regulation of furrow formation and this involvement may be fulfilled by regulating the dynamic structure of central spindle

    我們利用w7抑制cam活性，發現cam活性喪失會引起中區紡錘體結構異常；如用w7處理同步化于胞質分裂早期的細胞，可使細胞的分裂溝形成指數明顯下降，這表明cam的確參與調控了分裂溝的形成，而且可能是通過調控中區紡錘體的動態結構參與這一過程的。
  18. Though the technique of nuclejc transformation in plants has been developed and used widely, some problems in genetic information have not been resolved. for example, because the nucleic genome is so big and complicated that the integration sites and copies of foreign gene can not be controlled accurately, the expression of transferred genes is inefficient as a result of gene silencing or position effect. in nucleic transformation, furthermore, the transfer of multigene is difficult, and only after the prokaryotic genes undergo modification are they expressed in high plants

    植物的細胞核轉化技術已發展成熟並得到廣泛應用，但核基因組的遺傳轉化仍存在一系列至今尚未解決的問題：例如由於核基因組大，背景復雜，外源基因的整合位點和整合的拷貝數難以人為控制，造成鄭州大學2003年博士學位論文杜氏鹽藻（ dunaliellasalina ）葉綠體轉化研究外源基因表達效率低，容易出現基因失活、基因沉默、位置效應等現象;同時轉入多個基因時操作步驟過于復雜，所表達的原核基因必須經過修飾改造，環境安全難以保證等。
  19. Abstract : in order to clarify the location of the center for synchronized milk - ejection bursts of magnocellular oxytocin neurons in the hypothalamus, the bursts of these neurons were recorded extracellularly in lactating rats with selectively - cutting lesions of the middle brain or hypothalamus

    文摘:為探究授乳大鼠雙側下丘腦巨細胞催產素神經元同步化射乳反射爆發放電的中樞所在，我們採用雙微電極細胞外記錄技術，觀察了選擇性腦切割損毀后的大鼠雙側視上核內催產素神經元在仔鼠吸吮刺激下射乳反射爆發放電。
  20. Synchronization of hepg2 cells induced by tdr

    2細胞同步化
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